Assessment of Antimicrobial Therapy in EradicatingChlamydia muridarumin Research Mice: Immune Status and its Impact on Outcomes DOI Open Access

Michael B Palillo,

Sebastian E. Carrasco, Noah Mishkin

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 29, 2024

Abstract Chlamydia muridarum (Cm) is a moderately prevalent, gram-negative, intracellular bacterium that affects laboratory mice, causing subclinical to severe disease, depending on the host’s immune status. The effectiveness of various antibiotic regimens aimed at eradicating Cm in both immunodeficient and immunocompetent mice was evaluated. NSG were cohoused with Cm-shedding BALB/cJ for 14 days simulate natural exposure. Four groups 8 infected treated 7 either 0.08% sulfamethoxazole 0.016% trimethoprim (TMS) water, 0.0625% doxycycline feed, 0.124%/0.025% TMS or 0.12% amoxicillin feed. A control group provided standard water impact treatment gastrointestinal microbiota (GM) performed using next-generation shotgun sequencing last day treatment. Amoxicillin had negligible effects GM, while largest effect. All exhibited clinical including dehydration, hunched posture, >20% weight loss, dyspnea, leading euthanasia 21-40 post-treatment (32.6 ± 4.2 days; mean SD). Untreated controls euthanized 14-33 post-exposure (23.75 5.9 days). fecal PCR positive euthanasia. Histological evaluation revealed multifocal histiocytic neutrophilic bronchointerstitial pneumonia and/or bronchiolitis featuring prominent intralesional chlamydial inclusion bodies all mice. Subsequently, C57BL/6J, BALB/cJ, NOD.SCID, feed (BALB/cJ C57BL/6J) 21 (NSG NOD.SCID). NOD.SCID negative by post-treatment, remained clinically normal no evidence infection necropsy, euthanized. While these findings highlight difficulties from highly eradication immunocompromised antibiotics feasible.

Language: Английский

Vasoactive Intestinal Peptide Receptor, CRTH2, Antagonist Treatment Improves Eosinophil and Mast Cell-Mediated Esophageal Remodeling and Motility Dysfunction in Eosinophilic Esophagitis DOI Creative Commons
Chandra Sekhar Yadavalli, Sathisha Upparahalli Venkateshaiah, Alok Kumar Verma

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(4), P. 295 - 295

Published: Feb. 6, 2024

Background and Aims: Ultrasonography has shown that eosinophils accumulate in each segment of the esophageal mucosa human EoE, ultimately promoting motility dysfunction; however, no mechanistic evidence explains how or why this accumulation occurs. Methods: Quantitative PCR, ELISA, flow cytometry, immunostaining, immunofluorescence analyses were performed using antibodies specific to related antigens receptors. Results: In deep biopsies EoE patients, mast cells adjacent nerve cell-derived VIP segment. qRT-PCR analysis revealed five- sixfold increases expression levels VIP, CRTH2, VAPC2 receptors proteins blood- tissue-accumulated cells. We also observed a significant correlation between mRNA CRTH2 eosinophil- VIPs (p < 0.05). provide eosinophil cell deficiency following antagonist treatment improves dysfunction chronic DOX-inducible CC10-IL-13 murine model experimental EoE. Conclusions: is novel therapeutic strategy for inflammatory cell-induced IL-13-induced

Language: Английский

Citations

0
Assessment of Antimicrobial Therapy in EradicatingChlamydia muridarumin Research Mice: Immune Status and its Impact on Outcomes DOI Open Access

Michael B Palillo,

Sebastian E. Carrasco, Noah Mishkin

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 29, 2024

Abstract Chlamydia muridarum (Cm) is a moderately prevalent, gram-negative, intracellular bacterium that affects laboratory mice, causing subclinical to severe disease, depending on the host’s immune status. The effectiveness of various antibiotic regimens aimed at eradicating Cm in both immunodeficient and immunocompetent mice was evaluated. NSG were cohoused with Cm-shedding BALB/cJ for 14 days simulate natural exposure. Four groups 8 infected treated 7 either 0.08% sulfamethoxazole 0.016% trimethoprim (TMS) water, 0.0625% doxycycline feed, 0.124%/0.025% TMS or 0.12% amoxicillin feed. A control group provided standard water impact treatment gastrointestinal microbiota (GM) performed using next-generation shotgun sequencing last day treatment. Amoxicillin had negligible effects GM, while largest effect. All exhibited clinical including dehydration, hunched posture, >20% weight loss, dyspnea, leading euthanasia 21-40 post-treatment (32.6 ± 4.2 days; mean SD). Untreated controls euthanized 14-33 post-exposure (23.75 5.9 days). fecal PCR positive euthanasia. Histological evaluation revealed multifocal histiocytic neutrophilic bronchointerstitial pneumonia and/or bronchiolitis featuring prominent intralesional chlamydial inclusion bodies all mice. Subsequently, C57BL/6J, BALB/cJ, NOD.SCID, feed (BALB/cJ C57BL/6J) 21 (NSG NOD.SCID). NOD.SCID negative by post-treatment, remained clinically normal no evidence infection necropsy, euthanized. While these findings highlight difficulties from highly eradication immunocompromised antibiotics feasible.

Language: Английский

Citations

0