Prolonged Chronic Cerebral Hypoperfusion Does not Exacerbate Tau Pathology in a Tauopathy Mouse Model DOI Creative Commons
Na Kyung Lee, Duk L. Na, Hee‐Jin Kim

et al.

Journal of Integrative Neuroscience, Journal Year: 2025, Volume and Issue: 24(2)

Published: Feb. 21, 2025

Background: Several preclinical studies have reported elevated levels of tau following the induction chronic cerebral hypoperfusion (CCH) in Alzheimer’s disease mouse models. The objective this study was to first induce CCH a model tauopathy over an extended period up 6 months and subsequently investigate effects on accumulation alterations transcriptome. Methods: Three-month-old P301S mice were randomly allocated either Sham or group. common carotid arteries (CCAs) group bilaterally implanted using 0.75-mm inner diameter ameroid constrictors. Prior surgery, Doppler ultrasound imaging acquired, with follow-up at 1, 3, postoperatively. Brain tissue samples obtained, hemispheres dissected divided for separate analysis. Result: No significant differences phosphorylated total protein found left cortical hippocampal lysates. Immunohistochemical staining right hemisphere revealed similar findings. Compared group, transcriptomic deconvolution reduction memory B cells (p = 0.029). Conclusion: To clarify pathology, more than one surgical method should be used future studies.

Language: Английский

Prolonged Chronic Cerebral Hypoperfusion Does not Exacerbate Tau Pathology in a Tauopathy Mouse Model DOI Creative Commons
Na Kyung Lee, Duk L. Na, Hee‐Jin Kim

et al.

Journal of Integrative Neuroscience, Journal Year: 2025, Volume and Issue: 24(2)

Published: Feb. 21, 2025

Background: Several preclinical studies have reported elevated levels of tau following the induction chronic cerebral hypoperfusion (CCH) in Alzheimer’s disease mouse models. The objective this study was to first induce CCH a model tauopathy over an extended period up 6 months and subsequently investigate effects on accumulation alterations transcriptome. Methods: Three-month-old P301S mice were randomly allocated either Sham or group. common carotid arteries (CCAs) group bilaterally implanted using 0.75-mm inner diameter ameroid constrictors. Prior surgery, Doppler ultrasound imaging acquired, with follow-up at 1, 3, postoperatively. Brain tissue samples obtained, hemispheres dissected divided for separate analysis. Result: No significant differences phosphorylated total protein found left cortical hippocampal lysates. Immunohistochemical staining right hemisphere revealed similar findings. Compared group, transcriptomic deconvolution reduction memory B cells (p = 0.029). Conclusion: To clarify pathology, more than one surgical method should be used future studies.

Language: Английский

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