Ursodeoxycholic and chenodeoxycholic bile acids alleviate endotoxininduced acute lung injury in rats by modulating aquaporin expression and pathways associated with apoptosis and inflammation DOI Creative Commons

Tatjana Milivojac,

Milkica Grabež,

Ljiljana Amidžić

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: March 6, 2025

Introduction This study aimed to investigate the anti-inflammatory, antioxidant, and anti-apoptotic properties of ursodeoxycholic (UDCA) chenodeoxycholic (CDCA) bile acids in a rat model endotoxin (lipopolysaccharide, LPS)-induced acute lung injury (ALI). Methods The included six groups Wistar rats exposed different pretreatments. control were pretreated with propylene glycol, solvent for acids, while other received UDCA or CDCA 10 days. On 10th day, an injection was given evaluate impact these Lung tissue sections analyzed by immunohistochemistry, targeting pro-inflammatory marker nuclear factor kappa B (NF-κB), B-cell lymphoma 2 (BCL-2), pro-apoptotic markers BCL-2-associated X protein (BAX) caspase 3, as well aquaporins 1 5 (AQP1 AQP5). Oxidative stress assessed bronchoalveolar lavage fluid (BALF). Results discussion demonstrates that can mitigate endotoxin-induced rats. These effects are achieved through modulation AQP1 AQP5 expression, reduction oxidative stress, regulation apoptotic pathways (BAX, BCL-2), attenuation activity NF-κB. Although results indicate significant association between expression proteins histopathological changes, potential influence additional factors cannot be excluded. findings suggest provide protection acting complex mechanisms involving inflammatory, oxidative, pathways.

Language: Английский

Ursodeoxycholic and chenodeoxycholic bile acids alleviate endotoxininduced acute lung injury in rats by modulating aquaporin expression and pathways associated with apoptosis and inflammation DOI Creative Commons

Tatjana Milivojac,

Milkica Grabež,

Ljiljana Amidžić

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: March 6, 2025

Introduction This study aimed to investigate the anti-inflammatory, antioxidant, and anti-apoptotic properties of ursodeoxycholic (UDCA) chenodeoxycholic (CDCA) bile acids in a rat model endotoxin (lipopolysaccharide, LPS)-induced acute lung injury (ALI). Methods The included six groups Wistar rats exposed different pretreatments. control were pretreated with propylene glycol, solvent for acids, while other received UDCA or CDCA 10 days. On 10th day, an injection was given evaluate impact these Lung tissue sections analyzed by immunohistochemistry, targeting pro-inflammatory marker nuclear factor kappa B (NF-κB), B-cell lymphoma 2 (BCL-2), pro-apoptotic markers BCL-2-associated X protein (BAX) caspase 3, as well aquaporins 1 5 (AQP1 AQP5). Oxidative stress assessed bronchoalveolar lavage fluid (BALF). Results discussion demonstrates that can mitigate endotoxin-induced rats. These effects are achieved through modulation AQP1 AQP5 expression, reduction oxidative stress, regulation apoptotic pathways (BAX, BCL-2), attenuation activity NF-κB. Although results indicate significant association between expression proteins histopathological changes, potential influence additional factors cannot be excluded. findings suggest provide protection acting complex mechanisms involving inflammatory, oxidative, pathways.

Language: Английский

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