Establishment and application of a triplex real-time PCR assay for detection of porcine circoviruses DOI Creative Commons
Ze Cheng, Zenglin Wang,

Licheng Quan

et al.

Frontiers in Veterinary Science, Journal Year: 2025, Volume and Issue: 12

Published: March 3, 2025

Porcine circovirus disease represents a prevalent ailment that is principally manifested by series of clinical symptoms, including reproductive disorders in sows and high mortality rates piglets. It has brought huge economic losses upon the global swine industry. Furthermore, symptoms triggered different genotypes porcine bear resemblance difficult to distinguish. Therefore, it essential establish rapid, accurate, time-efficient, high-throughput triplex real-time PCR differential diagnosis assay for detecting PCV2, PCV3, PCV4. In this study, specific primers probes were designed based on conserved sequences ORF1 The Real-Time was established optimized, which showed satisfactory specificity, sensitivity, repeatability, reproducibility. limit detection (LOD) determined 4.8×10 1 copies/μL. correlation coefficients R 2 exceeded 0.999, no cross-infection found with other viral pathogens. addition, both intra-repeatability inter-repeatability lower than 2%, further attests reliability stability assay. complete consistency results those commercial single-plex kits indicates accuracy. next applied detect 370 samples collected from 2023 2024 northern Anhui province China. positive rate PCV2 81.35% (301/370), PCV3 72.43% (268/370), co-infection 38.37% (142/370). However, PCV4 not detected. study provides valuable tool circovirus, facilitates epidemiological investigation

Language: Английский

Establishment and application of a triplex real-time PCR assay for detection of porcine circoviruses DOI Creative Commons
Ze Cheng, Zenglin Wang,

Licheng Quan

et al.

Frontiers in Veterinary Science, Journal Year: 2025, Volume and Issue: 12

Published: March 3, 2025

Porcine circovirus disease represents a prevalent ailment that is principally manifested by series of clinical symptoms, including reproductive disorders in sows and high mortality rates piglets. It has brought huge economic losses upon the global swine industry. Furthermore, symptoms triggered different genotypes porcine bear resemblance difficult to distinguish. Therefore, it essential establish rapid, accurate, time-efficient, high-throughput triplex real-time PCR differential diagnosis assay for detecting PCV2, PCV3, PCV4. In this study, specific primers probes were designed based on conserved sequences ORF1 The Real-Time was established optimized, which showed satisfactory specificity, sensitivity, repeatability, reproducibility. limit detection (LOD) determined 4.8×10 1 copies/μL. correlation coefficients R 2 exceeded 0.999, no cross-infection found with other viral pathogens. addition, both intra-repeatability inter-repeatability lower than 2%, further attests reliability stability assay. complete consistency results those commercial single-plex kits indicates accuracy. next applied detect 370 samples collected from 2023 2024 northern Anhui province China. positive rate PCV2 81.35% (301/370), PCV3 72.43% (268/370), co-infection 38.37% (142/370). However, PCV4 not detected. study provides valuable tool circovirus, facilitates epidemiological investigation

Language: Английский

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