Models for barrier understanding in health and disease in lab-on-a-chips DOI Creative Commons
J. Ponmozhi, S. Dhinakaran, Dorottya Kocsis

et al.

Tissue Barriers, Journal Year: 2023, Volume and Issue: 12(2)

Published: June 9, 2023

The maintenance of body homeostasis relies heavily on physiological barriers. Dysfunction these barriers can lead to various pathological processes, including increased exposure toxic materials and microorganisms. Various methods exist investigate barrier function in vivo vitro. To a highly reproducible manner, ethically, high throughput, researchers have turned non-animal techniques micro-scale technologies. In this comprehensive review, the authors summarize current applications organ-on-a-chip microfluidic devices study review covers blood-brain barrier, ocular barriers, dermal respiratory intestinal, hepatobiliary, renal/bladder under both healthy conditions. article then briefly presents placental/vaginal, tumour/multi-organ devices. Finally, discusses Computational Fluid Dynamics systems that integrate biological This provides concise yet informative overview state-of-the-art studies using

Language: Английский

Microfluidic Devices: A Tool for Nanoparticle Synthesis and Performance Evaluation DOI Creative Commons
Sara Gimondi, Helena Ferreira, Rui L. Reis

et al.

ACS Nano, Journal Year: 2023, Volume and Issue: 17(15), P. 14205 - 14228

Published: July 27, 2023

The use of nanoparticles (NPs) in nanomedicine holds great promise for the treatment diseases which conventional therapies present serious limitations. Additionally, NPs can drastically improve early diagnosis and follow-up many disorders. However, to harness their full capabilities, they must be precisely designed, produced, tested relevant models. Microfluidic systems simulate dynamic fluid flows, gradients, specific microenvironments, multiorgan complexes, providing an efficient cost-effective approach both synthesis screening. technologies allow under controlled conditions, enhancing batch-to-batch reproducibility. Moreover, due versatility microfluidic devices, it is possible generate customize endless platforms rapid vitro vivo screening NPs' performance. Indeed, devices show potential as advanced small organism manipulation immobilization. In this review, first we summarize major that synthesis. Next, will discuss most innovative enable mimicking environments well give insights into organism-on-a-chip promising application We conclude review with a critical assessment current challenges future directions impact field nanomedicine.

Language: Английский

Citations

125
Involvement of Astrocytes in the Formation, Maintenance, and Function of the Blood–Brain Barrier DOI Creative Commons
Gabriella Schiera, Carlo Maria Di Liegro,

Giuseppe Schirò

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(2), P. 150 - 150

Published: Jan. 12, 2024

The blood-brain barrier (BBB) is a fundamental structure that protects the composition of brain by determining which ions, metabolites, and nutrients are allowed to enter from blood or leave it towards circulation. BBB structurally composed layer capillary endothelial cells (BCECs) bound each other through tight junctions (TJs). However, its development as well maintenance properties controlled contact BCECs: pericytes, glial cells, even neurons themselves. Astrocytes seem, in particular, have very important role controlling most BBB. Here, we will focus on these latter since comprehension their roles physiology has been continuously expanding, including ability participate neurotransmission complex functions such learning memory. Accordingly, pathological conditions alter astrocytic can BBB's integrity, thus compromising many activities. In this review, also refer different kinds vitro models used study properties, evidencing modifications under conditions.

Language: Английский

Citations

40
Glioma diagnosis and therapy: Current challenges and nanomaterial-based solutions DOI
Tianzhong Li, Jingfeng Li, Zhi Chen

et al.

Journal of Controlled Release, Journal Year: 2022, Volume and Issue: 352, P. 338 - 370

Published: Oct. 28, 2022

Language: Английский

Citations

70
Blood–Brain Barrier Breakdown in Neuroinflammation: Current In Vitro Models DOI Open Access
Sarah E. Brandl, Markus Reindl

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(16), P. 12699 - 12699

Published: Aug. 11, 2023

The blood-brain barrier, which is formed by tightly interconnected microvascular endothelial cells, separates the brain from peripheral circulation. Together with other central nervous system-resident cell types, including pericytes and astrocytes, barrier forms neurovascular unit. Upon neuroinflammation, this becomes leaky, allowing molecules cells to enter potentially harm tissue of system. Despite significance animal models in research, they may not always adequately reflect human pathophysiology. Therefore, are needed. This review will provide an overview terms both health disease. It describe all key elements vitro explore how different compositions can be utilized effectively model a variety neuroinflammatory conditions. Furthermore, it existing types that used basic research study respective pathologies thus far.

Language: Английский

Citations

30
Vascular inflammation on a chip: A scalable platform for trans-endothelial electrical resistance and immune cell migration DOI Creative Commons
Haley Ehlers, Arnaud Nicolas,

Frederik Schavemaker

et al.

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: Jan. 24, 2023

The vasculature system plays a critical role in inflammation processes the body. Vascular inflammatory mechanisms are characterized by disruption of blood vessel wall permeability together with increased immune cell recruitment and migration. There is need to develop models that fully recapitulate changes vascular barrier response conditions. We developed scalable platform for parallel measurements trans epithelial electrical resistance (TEER) 64 perfused microfluidic HUVEC tubules under Over 250 where exposed Tumor necrosis factor alpha (TNFα) interferon gamma (INF-γ) or human peripheral mononuclear cells. was quantified based on TEER expression ICAM VE-cadherin. observed function presence both cytokines cells, decreased values, increase as well endothelial morphology. OrganoPlate 3-lane64 provide valuable tool studies an automation compatible manner. Continuous enable long term, sensitive assays studies. propose use our powerful modelling combination interaction can be used screen targets drugs treat chronic inflammation.

Language: Английский

Citations

28
Lab-on-a-chip models of the blood–brain barrier: evolution, problems, perspectives DOI
Mária A. Deli, Gergő Porkoláb, András Kincses

et al.

Lab on a Chip, Journal Year: 2024, Volume and Issue: 24(5), P. 1030 - 1063

Published: Jan. 1, 2024

A great progress has been made in the development and use of lab-on-a-chip devices to model study blood–brain barrier (BBB) last decade.

Language: Английский

Citations

11
Microphysiological Blood‐Brain Barrier Systems for Disease Modeling and Drug Development DOI

Atharva R. Mulay,

Ji‐Hyun Hwang, Deok‐Ho Kim

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(21)

Published: March 2, 2024

Abstract The blood‐brain barrier (BBB) is a highly controlled microenvironment that regulates the interactions between cerebral blood and brain tissue. Due to its selectivity, many therapeutics targeting various neurological disorders are not able penetrate into Pre‐clinical studies using animals other in vitro platforms have shown ability fully replicate human BBB leading failure of majority clinical trials. However, recent innovations ex vivo modeling called organs‐on‐chips potential create more accurate disease models for improved drug development. These microfluidic induce physiological stressors on cultured cells generate physiologically BBBs compared previous models. In this review, different approaches BBBs‐on‐chips explored alongside their application therapeutic efficacy. Additionally, use delivery discussed, advances linking onto multiorgan mimic organ crosstalk reviewed.

Language: Английский

Citations

10
Unraveling brain diseases: The promise of brain-on-a-chip models DOI Creative Commons

Letizia Cerutti,

Martina Brofiga

Journal of Neuroscience Methods, Journal Year: 2024, Volume and Issue: 405, P. 110105 - 110105

Published: March 7, 2024

Brain disorders, encompassing a wide spectrum of neurological and psychiatric conditions, present formidable challenge in modern medicine. Despite decades research, the intricate complexity human brain still eludes comprehensive understanding, impeding development effective treatments. Recent advancements microfluidics tissue engineering have led to innovative platforms known as "Brain-on-a-Chip" (BoC) i.e., advanced vitro systems that aim replicate microenvironment with highest possible fidelity. This technology offers promising test-bed for studying disorders at cellular network levels, providing insights into disease mechanisms, drug screening, and, perspective, personalized therapeutic strategies. In this review, we provide an overview BoC models developed over years model understand onset progression some most severe terms incidence debilitation (stroke, Parkinson's, Alzheimer's, epilepsy). We also report cutting-edge approaches whose effects were evaluated by means these technologies. Finally, discuss potential challenges, future perspectives models.

Language: Английский

Citations

10
Vascularized organoid-on-a-chip: design, imaging, and analysis DOI
Tingting Yu,

Qihang Yang,

Bo Peng

et al.

Angiogenesis, Journal Year: 2024, Volume and Issue: 27(2), P. 147 - 172

Published: Feb. 26, 2024

Language: Английский

Citations

9
Human BBB-on-a-chip reveals barrier disruption, endothelial inflammation, and T cell migration under neuroinflammatory conditions DOI Creative Commons
Arya Lekshmi Nair,

Linda Groenendijk,

Roos Overdevest

et al.

Frontiers in Molecular Neuroscience, Journal Year: 2023, Volume and Issue: 16

Published: Sept. 25, 2023

The blood-brain barrier (BBB) is a highly selective that ensures homeostatic environment for the central nervous system (CNS). BBB dysfunction, inflammation, and immune cell infiltration are hallmarks of many CNS disorders, including multiple sclerosis stroke. Physiologically relevant human in vitro models essential to improve our understanding its function health disease, identify novel drug targets, assess potential new therapies. We present BBB-on-a-chip model comprising brain microvascular endothelial cells (HBMECs) cultured microfluidic platform allows parallel culture 40 chips. In each chip, perfused HBMEC vessel was grown against an extracellular matrix gel membrane-free manner. BBBs-on-chips were exposed varying concentrations pro-inflammatory cytokines tumor necrosis factor alpha (TNFα) interleukin-1 beta (IL-1β) mimic inflammation. effect inflammatory conditions studied by assessing BBBs-on-chips' function, morphology, expression adhesion molecules. Primary T through lumen study adhesion, extravasation, migration. Under conditions, showed decreased trans-endothelial electrical resistance (TEER), increased permeability sodium fluorescein, aberrant morphology concentration-dependent Moreover, we observed molecules concomitant monocyte adhesion. extravasated from inflamed blood vessels migrated towards C-X-C Motif Chemokine Ligand 12 (CXCL12) gradient. significantly reduced trend migration presence Natalizumab, antibody blocks very late antigen-4 (VLA-4) used treatment sclerosis. conclusion, demonstrate high-throughput can be neuroinflammation anti-inflammatory barrier-restoring interventions fight neurological disorders.

Language: Английский

Citations

21