Neuroprotective Effects of Qi Jing Wan and Its Active Ingredient Diosgenin Against Cognitive Impairment in Plateau Hypoxia DOI Creative Commons

Tiantian Xia,

Ziqiao Yan, Pan Shen

et al.

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(5), P. 738 - 738

Published: May 17, 2025

Background/Objectives: High-altitude environments have a significant detrimental impact on the cognitive functions of brain. Qi Jing Wan (QJW), traditional herbal formula composed Angelica sinensis, Astragalus membranaceus, and Rhizoma Polygonati Odorati, has demonstrated potential efficacy in treating disorders. However, its effects dysfunction plateau hypoxic remain unclear. Methods: In this study, acute chronic impairment mouse models were constructed to investigate preventive therapeutic QJW active ingredient, diosgenin (Dio). Behavioral experiments conducted assess learning memory mice. Morphological changes hippocampal neurons synapses assessed, microglial activation inflammatory factor levels measured evaluate brain damage. Potential ingredients capable crossing blood–brain barrier identified through chemical composition analysis network database screening, followed by validation animal organoid experiments. Transcriptomics analysis, immunofluorescence staining, molecular docking techniques employed explore underlying mechanisms. Results: significantly enhanced abilities model mice, reduced structural damage neurons, restored NeuN expression, inhibited activation, improved synaptic revealed that Dio alleviated protected function regulating expression PDE4C. Conclusions: These findings indicate ingredient effectively mitigate injury prevent high-altitude environments.

Language: Английский

Neuroprotective Effects of Qi Jing Wan and Its Active Ingredient Diosgenin Against Cognitive Impairment in Plateau Hypoxia DOI Creative Commons

Tiantian Xia,

Ziqiao Yan, Pan Shen

et al.

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(5), P. 738 - 738

Published: May 17, 2025

Background/Objectives: High-altitude environments have a significant detrimental impact on the cognitive functions of brain. Qi Jing Wan (QJW), traditional herbal formula composed Angelica sinensis, Astragalus membranaceus, and Rhizoma Polygonati Odorati, has demonstrated potential efficacy in treating disorders. However, its effects dysfunction plateau hypoxic remain unclear. Methods: In this study, acute chronic impairment mouse models were constructed to investigate preventive therapeutic QJW active ingredient, diosgenin (Dio). Behavioral experiments conducted assess learning memory mice. Morphological changes hippocampal neurons synapses assessed, microglial activation inflammatory factor levels measured evaluate brain damage. Potential ingredients capable crossing blood–brain barrier identified through chemical composition analysis network database screening, followed by validation animal organoid experiments. Transcriptomics analysis, immunofluorescence staining, molecular docking techniques employed explore underlying mechanisms. Results: significantly enhanced abilities model mice, reduced structural damage neurons, restored NeuN expression, inhibited activation, improved synaptic revealed that Dio alleviated protected function regulating expression PDE4C. Conclusions: These findings indicate ingredient effectively mitigate injury prevent high-altitude environments.

Language: Английский

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