Nanoparticles in drinking water: Assessing health risks and regulatory challenges

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 949, P. 174940 - 174940

Published: July 22, 2024

Language: Английский

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An insight into impact of nanomaterials toxicity on human health

PeerJ, Journal Year: 2024, Volume and Issue: 12, P. e17807 - e17807

Published: Sept. 30, 2024

In recent years, advances in nanotechnology have significantly influenced electronics manufacturing, industrial processes, and medical research. Various industries seen a surge the use of nanomaterials. However, several researchers raised alarm about toxicological nature nanomaterials, which appear to be quite different from their crude forms. This altered can attributed unique physicochemical profile. They adversely affect human health environment. Nanomaterials that been released into environment tend accumulate over time cause significant impact on ecosystem organisms with adverse effects. Increased nanoparticles has led increased exposure daily lives, making them more vulnerable nanoparticle toxicity. Because small size, nanomaterials readily cross biological membranes enter cells, tissues, organs. Therefore, effect is particular concern. The effects mechanisms action are being researched worldwide. Technological also support monitoring new marketed for household purposes. It challenging area because exceptional properties updated review focuses diverse perspective We discussed types physiochemical responsible toxicity, routes exposure, bio-distribution, mechanism includes various

Language: Английский

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In vitro cell-transforming potential of secondary polyethylene terephthalate and polylactic acid nanoplastics

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 469, P. 134030 - 134030

Published: March 13, 2024

Continuous exposure to plastic pollutants may have serious consequences on human health. However, most toxicity assessments focus non-environmentally relevant particles and rarely investigate long-term effects such as cancer induction. The present study assessed the carcinogenic potential of two secondary nanoplastics: polyethylene terephthalate (PET) generated from bottles, a biodegradable polylactic acid material, respective examples environmentally existing new bioplastics. Pristine polystyrene nanoplastics were also included for comparison. A broad concentration range (6.25 – 200 μg/mL) each nanoplastic was tested in both initiation promotion conditions regulatory assessment-accepted vitro Bhas 42 cell transformation assay. Parallel cultures allowed confirmation efficient cellular internalisation three nanoplastics. Cell growth enhanced by assay, PET conditions. Moreover, number transformed foci significantly increased only highest which showed dose-dependency, indicating that nano can act non-genotoxic tumour promotor. Together, these findings support risk assessment raise concerns regarding whether real-life co-exposure other environmental result synergistic capacities. Secondary (NPLs) waste degradation bioaccumulate tissues cause cancer. Currently, information NPL carcinogenicity remains scarce. By using we first time NPLs, bioplastic, widely spread petroleum-based alternative, respectively. Notably, accelerated bioplastics has been associated with an release NPLs into environment. data will serve plastics.

Language: Английский

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Current status and future challenges of genotoxicity OECD Test Guidelines for nanomaterials: a workshop report

Mutagenesis, Journal Year: 2023, Volume and Issue: 38(4), P. 183 - 191

Published: May 26, 2023

Abstract Genotoxicity testing for nanomaterials remains challenging as standard approaches require some adaptation, and further development of nano-specific OECD Test Guidelines (TGs) Guidance Documents (GDs) are needed. However, the field genotoxicology continues to progress new approach methodologies (NAMs) being developed that could provide relevant information on range mechanisms genotoxic action may be imparted by nanomaterials. There is a recognition need implementation and/or adapted TGs, GDs, utilization NAMs within genotoxicity framework As such, requirements apply experimental data assessment in regulatory context neither clear, nor used practice. Thus, an international workshop with representatives from agencies, industry, government, academic scientists was convened discuss these issues. The expert discussion highlighted current deficiencies exist exposure regimes, insufficient physicochemical characterization, lack demonstration cell or tissue uptake internalization, limitations coverage modes action. Regarding latter aspect, consensus reached importance using support Also close engagement between regulators (i) clarity needs, (ii) improve acceptance use NAM-generated data, (iii) define how part weight evidence risk assessments.

Language: Английский

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Review of New Approach Methodologies for Application in Risk Assessment of Nanoparticles in the Food and Feed Sector: Status and Challenges

EFSA Supporting Publications, Journal Year: 2024, Volume and Issue: 21(9)

Published: Sept. 1, 2024

Abstract New Approach Methodologies (NAMs), broadly understood to include in silico, chemico, vitro and ex vivo methods, show great potential advancing risk assessment albeit their regulatory implementation is lagging. The EFSA Guidance on of nanomaterials (EFSA Nano‐RA) suggests nano‐specific best achieved through Integrated Approaches Testing Assessment (IATAs) with NAMs as the first choice generate new information. Integrating promises several advantages such a better human focus, more detailed insights into molecular mechanisms higher efficacy. However, applying NMs also poses considerable challenges issues related dispersion stability, dosimetry, agglomeration, dissolution, transformations or assay interferences. Significant efforts are being undertaken by standardisation organisations research projects establish various for NMs. Here thorough review provided covering that will be potentially useful food feed sector. It follows structure Nano‐RA expands it, where needed, support decision‐making selection NM assessment. begins an overview NAM‐frameworks, followed description individual including those relevant physicochemical characterisation, exposure hazard toxicodynamics toxicokinetics. focus concerning degradation/dissolution, genotoxicity, cytotoxicity, oxidative stress, (pro‐)inflammation, barrier integrity important endpoints initial screening according framework. As result, total 267 nano‐relevant NAMs, mostly “not validated” (with few notable exceptions), were included this review. Validation notwithstanding, could already prove reliable NMs, especially integrated approaches.

Language: Английский

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Identification of the Role of the HSP150 Gene on the Genotoxicity of Zinc Oxide Nanoparticles in Saccharomyces cerevisae

Journal of environmental chemical engineering, Journal Year: 2025, Volume and Issue: unknown, P. 116124 - 116124

Published: March 1, 2025

Language: Английский

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AI-Based Nanotoxicity Data Extraction and Prediction of Nanotoxicity

Computational and Structural Biotechnology Journal, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

With the growing use of nanomaterials (NMs), assessing their toxicity has become increasingly important. Among assessment methods, computational models for predicting nanotoxicity are emerging as alternatives to traditional in vitro and vivo assays, which involve high costs ethical concerns. As a result, qualitative quantitative importance data is now widely recognized. However, collecting large, high-quality both time-consuming labor-intensive. Artificial intelligence (AI)-based extraction techniques hold significant potential extracting organizing information from unstructured text. large language (LLMs) prompt engineering not been studied. In this study, we developed an AI-based automated pipeline facilitate efficient collection. The automation process was implemented using Python-based LangChain. We used 216 research articles training refine prompts evaluate LLM performance. Subsequently, most suitable with refined extract test data, 605 articles. performance on achieved F1D.E. (F1 score Data Extraction) ranging 84.6 % 87.6 across different LLMs. Furthermore, extracted dataset set, constructed machine learning (AutoML) that F1N.P. Nanotoxicity Prediction) exceeding 86.1 nanotoxicity. Additionally, assessed reliability applicability by comparing them terms ground truth, size, balance. This study highlights extraction, representing contribution research.

Language: Английский

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Pure drug nanomedicines - where we are?

Chinese Journal of Natural Medicines, Journal Year: 2025, Volume and Issue: 23(4), P. 385 - 409

Published: April 1, 2025

Language: Английский

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Genotoxicity of nanoparticles evaluated using the in vitro micronucleus assay, a review of recent data ^§

Journal of Environmental Science and Health Part C, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 19

Published: May 17, 2025

The in vitro micronucleus assay is a well-known and established component of the standard genotoxicity test battery. growing use nanomaterials around world along with human exposure to them has increased need for risk assessment regard safety, including potential genotoxicity. one most used tests evaluating nanomaterials. This review compiles studies since 2017 that performed assessments formation after cellular different Genotoxicity broad range silver, cerium, zinc, gold, nickel, cadmium, titanium, carbon, aluminum cell types were reviewed. While clear trends could be seen some nanoparticle like silver cerium nanoparticles, others gold nanoparticles showed mixed results. highlights usefulness effectiveness studying nanomaterials, part, also careful note guidelines should followed when conducting this order generate reliable quality-driven data.

Language: Английский

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Enzymatic Hydrolysis of Rutin: Evaluation of Kinetic Parameters and Anti-Proliferative, Mutagenic and Anti-Mutagenic Effects

Life, Journal Year: 2023, Volume and Issue: 13(2), P. 549 - 549

Published: Feb. 16, 2023

The bioavailability of glucoside flavonoids is influenced by the nature sugar, glucosides being absorbed faster than rhamnoglucosides, for example. One strategy to enhance enzymatic hydrolysis. In this study, some kinetic parameters hesperidinase-mediated hydrolysis rutin were evaluated using an UHPLC/QTOF-MSE analysis products a bioconversion reaction. resulting hydrolyzed rutins (after 4, 8 and 12 h reaction) submitted anti-proliferative Cytokinesis-Block Micronucleus (CBMN) assays in CHO-K1 cells. hydrolysis, final concentration quercetin-3-O-glucoside (Q3G) was directly proportional inversely reaction time. At (2.5 μg/mL), derivatives did not show mutagenic effect, except sample with higher content Q3G 4 rutin). Moreover, protected cells 92% time against methyl methanesulfonate-induced damage. These results suggested that anti-mutagenic effect might be related antioxidant cell death induction. Presenting good lipophilicity/hydrophilicity ratio, together activities, seemed promisor raw material development food supplements.

Language: Английский

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