Determination of the genotoxic effects of sweeteners, mannitol and lactitol DOI

Ebru Eker-Kartal,

Ece Avuloğlu-Yılmaz

Journal of Environmental Science and Health Part C, Journal Year: 2023, Volume and Issue: 41(3-4), P. 135 - 149

Published: Oct. 2, 2023

AbstractThe changes in dietary habit around the world have led to an increased use of additives food. The safety food has been a main focus research for many years due ongoing debate on their potential effects health. In this study, vitro genotoxic mannitol and lactitol, polyols used as sweetener additives, were evaluated using chromosomal aberrations (CAs) micronucleus (MN) assays human peripheral lymphocytes. Additionally, these sweeteners mitotic index (MI) nuclear division (NDI) investigated. Concentrations 500, 1000, 2000, 4000, 8000 μg/mL 250, 4000 lactitol used. results indicated that both did not affect CA MN frequency, cause significant change NDI at all treatment concentratoins. However, (except concentrations 500 1000 μg/mL) 250 significantly decreased MI compared control almost times. conclusion, it was observed effect lymphocytes vitro.Keywords: Mannitollactitolfood additivegenotoxicityhuman AcknowledgmentsThis study partially supported by Amasya University Research Fund under Project No. FMB-BAP 20-0459.Ethics approvalThe carried out with permission Clinical Ethics Committee (11.06.2020/42). Informed consent obtained from individual participants (2 female 1 male donor whole blood) included study.Author contributionsThis paper produced Ebru Eker-Kartal's Master's thesis. Ece Avuloglu-Yilmaz supervisor All authors contributed conception design. Experimental studies analysis performed Eker-Kartal Avuloglu-Yilmaz. first draft manuscript written commented previous versions manuscript. read approved final manuscript.Disclosure statementThe declare no competing interests.Data availability statementData could be available request.Additional informationFundingThis 20-0459.

Language: Английский

Adapting the in vitro micronucleus assay (OECD Test Guideline No. 487) for testing of manufactured nanomaterials: recommendations for best practices DOI Creative Commons
Michael J. Burgum,

Clarissa Ulrich,

N. Partosa

et al.

Mutagenesis, Journal Year: 2024, Volume and Issue: 39(3), P. 205 - 217

Published: March 18, 2024

The current Organisation for Economic Co-Operation and Development test guideline number 487 (OECD TG No. 487) provides instruction on how to conduct the in vitro micronucleus assay. This assay is one of gold standard approaches measuring mutagenicity items; however, it directed at testing low molecular weight molecules may not be appropriate particulate materials (e.g. engineered nanoparticles [ENPs]). study aimed adapt ENP underpins development an OECD guidance document. A harmonized, nano-specific protocol was generated evaluated by two independent laboratories. Cell lines utilized were human lymphoblastoid (TK6) cells, liver hepatocytes (HepG2) Chinese hamster lung fibroblast (V79) whole blood, buffy coat cells from healthy volunteers. These exposed reference ENPs Joint Research Council (JRC): SiO2 (RLS-0102), Au5nm Au30nm (RLS-03, RLS-010), CeO2 (NM212), BaSO4 (NM220). Tungsten carbide-cobalt (WC/Co) used as a trial positive control. chemical controls all cell cultures, but WC/Co only TK6 cells. In observed SiO2- both Au types. HepG2 showed sub-two-fold increases micronuclei. V79 no genotoxicity detected with materials. data confirmed that could tested harmonized protocol, additionally, concordant across laboratories suitable control when using Detailed recommendations are therefore provided ENP.

Language: Английский

Citations

5
Evaluation of the cyto- and genotoxicity of two types of cellulose nanomaterials using human intestinal cells and in vitro digestion simulation DOI Creative Commons

Nádia Vital,

Maria do Socorro de Lucena Cardoso,

Michel Kranendonk

et al.

Archives of Toxicology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 24, 2024

Abstract Emerging cellulose nanomaterials (CNMs) may have commercial impacts in multiple sectors, being their application particularly explored the food sector. Thus, potential adverse effects gastrointestinal tract should be evaluated before marketing. This work aimed to assess safety of two CNMs (CNF–TEMPO and CMF–ENZ) through investigation cytotoxicity, genotoxicity (comet micronucleus assays), capacity induce reactive oxygen species human intestinal cells, mutagenic effect using Hprt gene mutation assay. Each toxicity endpoint was analysed after cells exposure a concentration-range each CNM or its digested product, obtained by standardized static vitro digestion method. The results showed an absence cytotoxic up highest concentration tested (200 µg/mL 25 µg/mL, for non-digested CNMs, respectively). Of note, cytotoxicity control limited top samples (25 µg/mL) subsequent assays. Application battery assays that CNF–TEMPO CMF–ENZ do not mutations aneugenic/clastogenic effects. However, due observed DNA damage induction, genotoxic cannot excluded, even though seems attenuate effect. lowest induced chromosomal Caco-2 leading equivocal outcome. Ongoing research on epigenotoxic these strengthen lines evidence when ingested, paving way innovative industry.

Language: Английский

Citations

1
Determination of the genotoxic effects of sweeteners, mannitol and lactitol DOI

Ebru Eker-Kartal,

Ece Avuloğlu-Yılmaz

Journal of Environmental Science and Health Part C, Journal Year: 2023, Volume and Issue: 41(3-4), P. 135 - 149

Published: Oct. 2, 2023

AbstractThe changes in dietary habit around the world have led to an increased use of additives food. The safety food has been a main focus research for many years due ongoing debate on their potential effects health. In this study, vitro genotoxic mannitol and lactitol, polyols used as sweetener additives, were evaluated using chromosomal aberrations (CAs) micronucleus (MN) assays human peripheral lymphocytes. Additionally, these sweeteners mitotic index (MI) nuclear division (NDI) investigated. Concentrations 500, 1000, 2000, 4000, 8000 μg/mL 250, 4000 lactitol used. results indicated that both did not affect CA MN frequency, cause significant change NDI at all treatment concentratoins. However, (except concentrations 500 1000 μg/mL) 250 significantly decreased MI compared control almost times. conclusion, it was observed effect lymphocytes vitro.Keywords: Mannitollactitolfood additivegenotoxicityhuman AcknowledgmentsThis study partially supported by Amasya University Research Fund under Project No. FMB-BAP 20-0459.Ethics approvalThe carried out with permission Clinical Ethics Committee (11.06.2020/42). Informed consent obtained from individual participants (2 female 1 male donor whole blood) included study.Author contributionsThis paper produced Ebru Eker-Kartal's Master's thesis. Ece Avuloglu-Yilmaz supervisor All authors contributed conception design. Experimental studies analysis performed Eker-Kartal Avuloglu-Yilmaz. first draft manuscript written commented previous versions manuscript. read approved final manuscript.Disclosure statementThe declare no competing interests.Data availability statementData could be available request.Additional informationFundingThis 20-0459.

Language: Английский

Citations

0