A common regulatory switch controls a suite of C4 traits in multiple cell types DOI Creative Commons

Daniel Camo-Escobar,

Carlos Alcalá-Gutiérrez,

Ernesto Palafox-Figueroa

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 22, 2023

The C4 photosynthetic pathway provided a major advantage to plants growing in hot, dry environments, including the ancestors of our most productive crops. Two traits were essential for evolution this pathway: increased vein density and functionalization bundle sheath cells photosynthesis. Although GRAS transcriptional regulators, SHORT ROOT (SHR), have been implicated mediating leaf patterning both C3 species, little is known about what controls specialized features that mediate metabolism physiology. We show model monocot,

Language: Английский

Moisture-responsive root-branching pathways identified in diverse maize breeding germplasm DOI
Johannes Daniel Scharwies,

Taylor Clarke,

Zihao Zheng

et al.

Science, Journal Year: 2025, Volume and Issue: 387(6734), P. 666 - 673

Published: Feb. 6, 2025

Plants grow complex root systems to extract unevenly distributed resources from soils. Spatial differences in soil moisture are perceived by tips, leading the patterning of new branches toward available water a process called hydropatterning. Little is known about hydropatterning behavior and its genetic basis crop plants. Here, we developed an assay measure maize revealed substantial between tropical/subtropical temperate breeding germplasm that likely resulted divergent selection. Genetic analysis confirmed regulatory role auxin gaseous hormone ethylene locally inhibits branching air-exposed tissues. Our results demonstrate how distinct signaling pathways translate spatial patterns availability developmental programs determine architecture.

Language: Английский

Citations

3
Glutathione accelerates the cell cycle and cellular reprogramming in plant regeneration DOI
Laura R. Lee, Bruno Guillotin, Ramin Rahni

et al.

Developmental Cell, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

2
Dual role of BdMUTE during stomatal development in the model grass Brachypodium distachyon DOI
Roxane P. Spiegelhalder, Lea S. Berg, Tiago D. G. Nunes

et al.

Development, Journal Year: 2024, Volume and Issue: 151(20)

Published: Aug. 22, 2024

ABSTRACT Grasses form morphologically derived, four-celled stomata, where two dumbbell-shaped guard cells (GCs) are flanked by lateral subsidiary (SCs). This innovative enables rapid opening and closing kinetics efficient plant–atmosphere gas exchange. The mobile bHLH transcription factor MUTE is required for SC formation in grasses. Yet whether how also regulates GC development mobility recruitment unclear. Here, we transgenically impaired BdMUTE from to precursors the emerging model grass Brachypodium distachyon. Our data indicate that reduced severely affected spatiotemporal coordination of development. Furthermore, although has a cell-autonomous role division orientation, complete dumbbell morphogenesis GCs recruitment. Finally, leaf-level exchange measurements showed dosage-dependent complementation morphology was mirrored gradual physiological stomatal kinetics. Together, our work revealed dual regulating orientation recruitment, which turn stomata.

Language: Английский

Citations

5
Spatial transcriptomics of a lycophyte root sheds light on root evolution DOI Creative Commons

Xilan Yang,

Ward Poelmans,

Carolin Grones

et al.

Current Biology, Journal Year: 2023, Volume and Issue: 33(19), P. 4069 - 4084.e8

Published: Sept. 7, 2023

Language: Английский

Citations

12
Dual and spatially resolved drought responses in the Arabidopsis leaf mesophyll revealed by single‐cell transcriptomics DOI Creative Commons
Rubén Tenorio Berrío, Eline Verhelst, Thomas Eekhout

et al.

New Phytologist, Journal Year: 2025, Volume and Issue: unknown

Published: March 3, 2025

Summary Drought stress imposes severe challenges on agriculture by impacting crop performance. Understanding drought responses in plants at a cellular level is crucial first step toward engineering improved resilience. However, the molecular to are complex as they depend multiple factors, including severity of drought, profiled organ, its developmental stage or even cell types therein. Thus, deciphering transcriptional especially challenging. In this study, we investigated tissue‐specific mild (MD) young Arabidopsis thaliana (Arabidopsis) leaves using single‐cell RNA sequencing (scRNA‐seq). To preserve integrity during isolation, inhibited synthesis transcription inhibitor actinomycin D, and demonstrated benefits transcriptome fixation for studying level. We present curated validated atlas, comprising 50 797 high‐quality cells from almost all known leaf. All type annotations were with new library reporter lines. The data available broad community an intuitive tool browsable atlas ( http://www.single‐cell.be/plant/leaf‐drought ). show that mesophyll contains two spatially separated populations distinct drought: one enriched canonical abscisic acid‐related drought‐responsive genes, another genes involved iron starvation responses. Our study thus reveals dual adaptive mechanism leaf response MD provides valuable resource future research

Language: Английский

Citations

0
Studying plant vascular development using single-cell approaches DOI
Claudia von der Mark, Max Minne, Bert De Rybel

et al.

Current Opinion in Plant Biology, Journal Year: 2024, Volume and Issue: 78, P. 102526 - 102526

Published: March 12, 2024

Language: Английский

Citations

2
Maize genetic diversity identifies moisture-dependent root-branch signaling pathways DOI Open Access
Johannes Daniel Scharwies,

Taylor Clarke,

Zihao Zheng

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 27, 2024

Abstract Plants grow complex root systems to extract unevenly distributed resources from soils. Spatial differences in soil moisture are perceived by tips leading the patterning of new branches towards available water, a process called hydropatterning. Little is known about hydropatterning behavior and its genetic basis crops plants. Here, we develop an assay measure maize reveal substantial between tropical/subtropical temperate breeding germplasm that likely resulted divergent selection. Genetic dissection confirmed regulatory role auxin revealed gaseous hormone ethylene acts locally inhibit branching air-exposed tissues. These findings demonstrate crop relevance establish basis.

Language: Английский

Citations

2
Nuclear GSH import precedes coordinated cell cycle changes during regeneration DOI Creative Commons
Laura R. Lee, Bruno Guillotin, Ramin Rahni

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 28, 2023

The plasticity of plant cells underlies their wide capacity to regenerate, with increasing evidence in plants and animals implicating cell cycle dynamics cellular reprogramming. To investigate the during reprogramming, we developed a comprehensive set phase markers Arabidopsis root. Using single-cell RNA-seq profiles live imaging regeneration, found that subset near an ablation injury dramatically increases division rate by truncating G1. Cells G1 undergo transient nuclear peak glutathione (GSH) prior coordinated entry into S followed rapid divisions A symplastic block ground tissue impairs which is rescued exogenous GSH. We propose model GSH from outer tissues released upon licensing exit wound induce

Language: Английский

Citations

2
Fluorescence hybridization chain reaction enables localization of multiple molecular classes combined with plant cell ultrastructure DOI Open Access
Yunqing Yu, David Huss, Li Mao

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 31, 2024

ABSTRACT Background Recent developments in hybridization chain reaction (HCR) have enabled robust simultaneous localization of multiple mRNA transcripts using fluorescence situ (FISH). Once split initiator oligonucleotide probes bind their target mRNA, HCR uses DNA base-pairing fluorophore-labeled hairpin sets to self-assemble into large polymers, amplifying the signal and reducing non-specific background. Few studies applied plants, despite its demonstrated utility whole mount animal tissues cell culture. Our aim was optimize this technique for sectioned plant embedded with paraffin methacrylate resins, test combination immunolocalization subsequent correlation ultrastructure scanning electron microscopy. Results Application 10 µm sections 17-day-old Setaria viridis (green millet) inflorescences confocal microscopy revealed that transcription factor KNOTTED 1 ( KN1 ) were localized developing floret meristem vascular tissue while SHATTERING SH1 MYB26 co-localized breakpoint below floral structures (the abscission zone). We also used de-embedment 1.5 0.5 3-day-old Arabidopsis thaliana seedlings show specific CHLOROPHYLL BINDING FACTOR a/b CAB1 highly expressed photosynthetic ELONGATION ALPHA EF1 α meristematic shoot apex. The housekeeping gene ACTIN7 ACT7 more uniformly distributed reduced signals lattice structured-illumination followed by backscattered imaging thus demonstrating feasibility correlating fluorescent ultrastructure. Conclusion successfully adapted use both on diverse two model organisms, allowing concurrent cellular subcellular mRNAs, antibodies other affinity probe classes. mild conditions made it amenable observe immunofluorescence same section. De-embedded semi-thin compatible correlative approaches. protocol provides numerous practical tips successful labeling microscopy-compatible, material.

Language: Английский

Citations

0
Quantitative RNA spatial profiling using single-molecule RNA FISH on plant tissue cryosections DOI Creative Commons
Xue Zhang, Alejandro Fonseca, Konstantin Kutashev

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 9, 2024

ABSTRACT Single-molecule fluorescence in situ hybridization (smFISH) has emerged as a powerful tool to study gene expression dynamics with unparalleled precision and spatial resolution variety of biological systems. Recent advancements have expanded its application encompass plant studies, yet demand persists for simple robust smFISH method adapted tissue sections. Here, we present an optimized protocol (cryo-smFISH) visualizing quantifying single mRNA molecules cryosections. This exhibits remarkable sensitivity, capable detecting low-expression transcripts, including long non-coding RNAs. Integrating deep learning-based algorithm our image analysis pipeline, enables us assign RNA abundance precisely nuclear cytoplasmic compartments. Compatibility Immunofluorescence also allows endogenous proteins be visualized quantified simultaneously. Finally, this presents the first time use single-cell sequencing (scRNA-seq) validation plants. By extending cryosections, even broader community scientists will able exploit multiple potentials quantitative transcript at cellular subcellular resolutions.

Language: Английский

Citations

0