A common regulatory switch controls a suite of C4 traits in multiple cell types DOI Creative Commons

Daniel Camo-Escobar,

Carlos Alcalá-Gutiérrez,

Ernesto Palafox-Figueroa

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 22, 2023

The C4 photosynthetic pathway provided a major advantage to plants growing in hot, dry environments, including the ancestors of our most productive crops. Two traits were essential for evolution this pathway: increased vein density and functionalization bundle sheath cells photosynthesis. Although GRAS transcriptional regulators, SHORT ROOT (SHR), have been implicated mediating leaf patterning both C3 species, little is known about what controls specialized features that mediate metabolism physiology. We show model monocot,

Language: Английский

Dual and spatially resolved drought responses in the Arabidopsis leaf mesophyll revealed by single-cell transcriptomics DOI Creative Commons
Rubén Tenorio Berrío, Eline Verhelst, Thomas Eekhout

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 30, 2024

ABSTRACT Drought stress imposes severe challenges on agriculture by impacting crop performance. Understanding drought responses in plants at a cellular level is crucial first step towards engineering improved resilience. However, the molecular to are complex as they depend multiple factors including severity of drought, profiled organ, its developmental stage or even cell types therein. Thus, deciphering transcriptional specially challenging. Here, we investigated tissue-specific mild young Arabidopsis thaliana (Arabidopsis) leaves using single-cell RNA sequencing (scRNA-seq). To preserve integrity during isolation, inhibited synthesis transcription inhibitor actinomycin D, demonstrating benefits transcriptome fixation for studying level. We present curated and validated atlas comprising 50,797 high-quality cells from almost all known leaf. show that mesophyll contains two spatially separated populations with distinct drought: one enriched canonical abscisic acid-related drought-responsive genes, another depicting iron starvation responses. Our study thus reveals dual adaptive mechanism leaf response provides valuable resource future research

Language: Английский

Citations

0
Light and high temperatures control epigenomic and epitranscriptomic events in Arabidopsis DOI Creative Commons
Tianyuan Xu, Eirini Patitaki, Anna Zioutopoulou

et al.

Current Opinion in Plant Biology, Journal Year: 2024, Volume and Issue: 83, P. 102668 - 102668

Published: Nov. 24, 2024

Language: Английский

Citations

0
A common regulatory switch controls a suite of C4 traits in multiple cell types DOI Creative Commons

Daniel Camo-Escobar,

Carlos Alcalá-Gutiérrez,

Ernesto Palafox-Figueroa

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 22, 2023

The C4 photosynthetic pathway provided a major advantage to plants growing in hot, dry environments, including the ancestors of our most productive crops. Two traits were essential for evolution this pathway: increased vein density and functionalization bundle sheath cells photosynthesis. Although GRAS transcriptional regulators, SHORT ROOT (SHR), have been implicated mediating leaf patterning both C3 species, little is known about what controls specialized features that mediate metabolism physiology. We show model monocot,

Language: Английский

Citations

1