Harnessing promoter elements to enhance gene editing in plants: perspectives and advances

Plant Biotechnology Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 27, 2025

Summary Genome‐edited plants, endowed with climate‐smart traits, have been promoted as tools for strengthening resilience against climate change. Successful plant gene editing (GE) requires precise regulation of the GE machinery, a process controlled by promoters, which drives its transcription through interactions factors (TFs) and RNA polymerase. While constitutive promoters are extensively used in constructs, their limitations highlight need alternative approaches. This review emphasizes promise tissue/organ specific well inducible enable targeted spatiotemporal manner no effects on other tissues. Advances synthetic biology paved way creation offering refined control over expression augmenting potential GE. The integration these novel systems presents significant opportunities conditional genome editing. Moreover, advent bioinformatic artificial intelligence is revolutionizing characterization regulatory elements, enhancing our understanding roles plants. Thus, this provides insights into strategic use promoter to enhance precision, efficiency specificity GE, setting stage innovative crop improvement strategies.

Language: Английский

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CRISPR/Cas in Grapevine Genome Editing: The Best Is Yet to Come

Horticulturae, Journal Year: 2024, Volume and Issue: 10(9), P. 965 - 965

Published: Sept. 11, 2024

The advent of Clustered Regularly Interspaced Palindromic Repeat (CRISPR)/CRISPR-associated (Cas) proteins as a revolutionary innovation in genome editing has greatly promoted targeted modification and trait improvement most plant species. For grapevine (Vitis vinifera L.), perennial woody species, CRISPR/Cas is an extremely promising technique for genetic short period. Advances have been achieved by using CRISPR technology recent years, which promises to accelerate grapevine. In this review, we describe the development advances CRISPR/Cas9 its orthologs variants. We summarize applications discuss challenges facing well possible strategies that could be used improve addition, outline future perspectives model system, precise editing, accelerated improvement, transgene-free editing. believe will play more important role exciting bright expected economically significant

Language: Английский

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A New Approach for CRISPR/Cas9 Editing and Selection of Pathogen-Resistant Plant Cells of Wine Grape cv. ‘Merlot’

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(18), P. 10011 - 10011

Published: Sept. 17, 2024

Grape is one of the most economically significant berry crops. Owing to biological characteristics grapes, such as long juvenile period (5-8 years), high degree genome heterozygosity, and frequent occurrence inbreeding depression, homozygosity during crossbreeding leads loss varietal viability. CRISPR/Cas editing has become tool choice for improving elite technical grape varieties. This study provides first evidence a decrease in total fraction phenolic compounds an increase concentration peroxide callus cells upon addition chitosan culture medium. These previously unreported metabolic features response have been described used time probability selecting plant with

Language: Английский

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Publisher Correction: New improvements in grapevine genome editing: high efficiency biallelic homozygous knock-out from regenerated plantlets by using an optimized zCas9i

Plant Methods, Journal Year: 2024, Volume and Issue: 20(1)

Published: June 14, 2024

Language: Английский

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Generation of a collection of MYB mutant lines via pooled CRISPR-Cas9 in grape

Journal of Integrative Agriculture, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

Language: Английский

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