Plants, Journal Year: 2024, Volume and Issue: 13(22), P. 3251 - 3251
Published: Nov. 20, 2024
Plants of the subfamily Amaryllidoideae are a source unique and bioactive alkaloids called Amaryllidaceae alkaloids. The study their anticancer potential has intensified in recent years. This work aims to locate characterize profile cytotoxic biosynthesized stored different tissues
Language: Английский
Journal of Liposome Research, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 15
Published: Feb. 2, 2025
Personalized treatment strategies have greatly improved the efficacy of anticancer drugs. Nanocarriers, especially liposomes, function as excellent platform for delivery both hydrophilic and hydrophobic agents. iRGD is a peptide composed 9-amino acid denoted (iRGDP), enhances selective intratumoral Trastuzumab (TMAB), mainly targets HER2-positive advanced stage breast cancer an FDA-approved monoclonal antibody. Gefitinib (GEB) drug, effective against metastatic (MBC), while Lycorine hydrochloride (LCOH), naturally derived compound, possess anti-inflammatory properties. This research emphasizing on preparation GEB LCOH-entrapped TPGS-COOH coated-liposomes, camouflaged with antibody (TMAB) cyclic (iRGDP) targeted in MBC therapy. The developed multifunctional liposomes were studied extensive vitro cell line studies MCF-7 cells. half-maximum inhibitory concentration (IC-50) values LCOH co-loaded single functionalized liposome (SFL) (iRGDP-LiP, TMAB-LiP) dual-functionalized (DFL) (iRGDP-TMAB-LiP) cells 1.04 ± 0.023 μg/mL, 0.71 0.018 0.56 0.028 respectively. Inverted confocal laser scanning microscopy (ICLSM) revealed enhanced cellular internalization SFL DFL-treated groups tagged coumarin-6 rhodamine-B dye compared to conventional liposome. scratch assay marked reduction migration, DAPI staining confirmed nuclear condensation (NC) fragmentation (NF) groups. Moreover, flow cytometry demonstrated early late apoptosis DFL These findings indicate that holds promise multifaceted therapeutic approach
Language: Английский
Citations
2
Cancers, Journal Year: 2025, Volume and Issue: 17(5), P. 718 - 718
Published: Feb. 20, 2025
Tumor metastasis and poor drug efficacy are two of the most common causes therapeutic failure in cancer patients. The underlying molecular mechanism requires further exploration, novel effective curative strategies urgently needed. Nature is a rich source drugs, Lycorine hydrochloride (Lyc.HCL) natural alkaloid with tremendous potential. However, mechanisms its antitumor activity still unknown. In current study, we investigated effects Lyc.HCL against esophageal squamous cell carcinomas (ESCCs), which pose serious threats to human life. An MTS assay clone formation were used assess viability ESCC lines after treatment vitro. Apoptosis cycle regulation analyzed using flow cytometry. Wound healing Transwell assays analyze migration, while invasion was Matrigel assay. We detected expression tripartite motif-containing 22 (TRIM22) through immunohistochemistry Western blotting. A docking experiment performed explore targets Lyc.HCL. levels Janus kinase 2 (JAK2)/signal transducer activator transcription 3 (STAT3) phosphoinositide 3-kinase (PI3K)/protein B (AKT)/extracellular signal-regulated (Erk) pathway components rescue determine potential role TRIM22. addition, explored vivo anti-ESCC by it treat tumor-bearing mice. found inhibit carcinoma proliferation both vitro blocking at G2 phase, inhibiting migration invasion. that TRIM22 protein highly expressed ESCCs but not normal tissue. directly targeted TRIM22, decreasing JAK2/STAT3 Erk signaling pathways, vivo. Using animal experiments, observed depletion delayed tumor growth, this effect significantly reversed upon overexpression. Taken together, these findings demonstrate can effectively suppress targeting regulating pathways. These results suggest may serve as for ESCC, emerging promising target treatment.
Language: Английский
Citations
0
Cells, Journal Year: 2025, Volume and Issue: 14(7), P. 525 - 525
Published: April 1, 2025
Alzheimer’s disease (AD) is a multifactorial neurodegenerative disorder characterized by metabolic dysregulation, oxidative stress, amyloid-β (Aβ) aggregation, metal dyshomeostasis, and mitochondrial dysfunction. Current treatments provide only symptomatic relief, highlighting the need for novel therapeutic strategies. This study investigates effects of alkaloids galantamine (GAL) lycorine (LYC) in differentiated SH-SY5Y neuroblastoma cells, an established vitro model AD, which acquire neuronal phenotype upon differentiation. Using untargeted targeted NMR-based metabolomics combined with multivariate statistical analysis, we analyzed extracellular profiles under basal conditions following Aβ42 exposure, both presence absence GAL LYC. Our findings reveal distinct responses to Aβ toxicity, significant alterations pyruvate glutamine metabolism. Both LYC contributed restoration lysine homeostasis, but had more pronounced effect, better sustaining cellular energy balance function. Unlike LYC, treatment was associated accumulation, response between two compounds. These variations may reflect mechanisms action, potentially influencing their roles counteracting Aβ-induced toxicity. highlights value profiling assessing neuroprotective agents reinforces potential natural this context.
Language: Английский
Citations
0
Purinergic Signalling, Journal Year: 2025, Volume and Issue: unknown
Published: May 13, 2025
Language: Английский
Citations
0
European Journal of Medicinal Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 117759 - 117759
Published: May 1, 2025
Language: Английский
Citations
0
Plants, Journal Year: 2024, Volume and Issue: 13(22), P. 3251 - 3251
Published: Nov. 20, 2024
Plants of the subfamily Amaryllidoideae are a source unique and bioactive alkaloids called Amaryllidaceae alkaloids. The study their anticancer potential has intensified in recent years. This work aims to locate characterize profile cytotoxic biosynthesized stored different tissues
Language: Английский
Citations
0