International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3169 - 3169
Published: March 29, 2025
Circadian rhythm disruption is a modifiable risk factor for Alzheimer’s disease (AD) progression, marked by neuroinflammation, oxidative stress, and amyloid-β (Aβ) accumulation. Hypoxia-inducible 3α (Hif3α) has emerged as key regulator of inflammatory pathways. To evaluate the impacts circadian on AD progression investigate therapeutic potential bamboo leaf flavonoids (BLFs), C57BL/6N mice (normal mice) APP/PS1 transgenic (AD were exposed to via randomized light exposure in vivo model. Then, BLFs administered assess effects organ damage. Next, Nissl body staining Aβ protein immunohistochemistry performed brain pathology. Through transcriptome sequencing, factors related pathway screened out. In vitro, molecular mechanisms explored PC12 cells treated with Aβ42 Hif3α siRNA fragments. Results demonstrated that increased stress early liver kidney damage degrees, greater severity mice. partially reversed reduced deposition. Transcriptome analysis revealed upregulation circadian-disrupted mice, linked inflammation stress. knockdown normalized expression, which could be regulated suppressed progression. conclusion, exacerbated regulating Hif3α/Rab7/TNFα/IL1β pathway. offered neuroprotection roles mitigating damage, highlighting promising target therapy biomarker development.
Language: Английский