Decellularization and Enzymatic Digestion Methods to Enhance ECM Protein Detection via MALDI-MS Imaging

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 3, 2025

Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) has been used to generate spatial maps of lipids, metabolites, peptides, proteins, and glycans in tissues; however, its use for mapping extracellular matrix (ECM) protein distributions is underexplored. ECM proteins play a major role various pathological conditions, changes their affect the function morphology cells within tissues. detection challenging because they are large, insoluble, undergo post-translational modifications, such as glycosylation. We describe here decellularization tissue sections coupled with serial enzymatic digestions PNGaseF trypsin improve MALDI-MSI without disrupting architecture. Decellularization leads 3-fold increase number that measured by MALDI-MSI. also introduce binary colocalization method identification, which increases confidently detected. Together, these methods enhance

Language: Английский

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Decellularized Liver Matrices for Expanding the Donor Pool—An Evaluation of Existing Protocols and Future Trends

Biomolecules, Journal Year: 2025, Volume and Issue: 15(1), P. 98 - 98

Published: Jan. 10, 2025

Liver transplantation is the only curative option for end-stage liver disease and necessary an increasing number of patients with advanced primary or secondary cancer. Many patient groups can benefit from this treatment, however shortage grafts remains unsolved problem. bioengineering offers a promising method expanding donor pool through production acellular scaffolds that be seeded recipient cells. Decellularization protocols involve removal cells using various chemical, physical, enzymatic steps to create collagenous network provides support introduced future vascular biliary beds. However, causes varying degrees matrix damage, affect cell seeding organ performance. The main objective review present existing techniques producing decellularized livers, emphasis on assessment definition acellularity. agents are discussed, standard process evaluated. We also introduce concept stepwise during decellularization cycles. This may lead shorter detergent exposure times less scaffold damage. introduction apoptosis induction in field engineering provide valuable alternative long perfusion protocols, which significant A thorough understanding action factors influencing final composition essential produce biocompatible matrix, basis further studies regarding recellularization retransplantation.

Language: Английский

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Application of decellularized tissue for soft-hard interregional regeneration

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13

Published: April 16, 2025

Decellularized tissue refers to extracellular matrix (ECM) derived from living by removing the cellular components and is used for regeneration. Various decellularized sheets powders, such as dermis, urinary bladder matrix, small intestinal submucosa, have been clinically covering prosthetic materials. Moreover, there growing interest in use of soft-hard interregional regeneration, including ligament-bone, tendon-bone, periodontal ligament-bone interfaces. The focus these applications lies mechanical properties tissue. ligaments tendons developed using various decellularization methods, with a on maintaining their shape properties, applied orthotopically or ectopically tendons. In interface, it suggested that ligament tendon are regenerated through migration rearrangement host cells, which referred "in situ regeneration." It also proposed can be prepare complex structure tissue, consists an ECM cell populations gradual change. this case, soft tissues ligaments, tendons, pericardium, others fabricated modified hard mimic review, we present detailed discussion regeneration

Language: Английский

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Recent advances in extracellular matrix manipulation for kidney organoid research

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 6, 2024

The kidney plays a crucial role in maintaining the body’s microenvironment homeostasis. However, current treatment options and therapeutic agents for chronic disease (CKD) are limited. Fortunately, advent of organoids has introduced novel vitro model studying diseases drug screening. Despite significant efforts been leveraged to mimic spatial-temporal dynamics fetal renal development various types organoids, there is still discrepancy cell maturity compared native tissue. extracellular matrix (ECM) regulating cellular signaling, which ultimately affects fate decision. As result, ECM can refine promoting their efficient differentiation maturation. This review examines existing techniques culturing evaluates strengths weaknesses assesses advancements limitations associated with utilization organoid culture. Additionally, it presents discussion on constructing specific physiological pathological microenvironments using decellularized during certain developmental stages or occurrences, aiding models.

Language: Английский

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