Analgesia by intrathecal delta-9-tetrahydrocannabinol is dependent on Cav3.2 calcium channels DOI Creative Commons

Vinícius de Maria Gadotti,

Flavia Tasmin Techera Antunes, Gerald W. Zamponi

et al.

Molecular Brain, Journal Year: 2023, Volume and Issue: 16(1)

Published: May 25, 2023

Abstract Delta-9-tetrahydrocannabinol (Δ 9 -THC) is known to produce systemic analgesia that involves CB 1 and 2 cannabinoid receptors. However, there compelling evidence Δ -THC can potently inhibit Cav3.2T-type calcium channels which are highly expressed in dorsal root ganglion neurons the horn of spinal cord. Here, we investigated whether produced by Cav3.2 vis a We show spinally delivered dose-dependent long-lasting mechanical anti-hyperalgesia neuropathic mice, showed potent analgesic effects models inflammatory pain induced formalin or Complete Freund’s Adjuvant (CFA) injection into hind paw, with latter showing no overt sex differences. The mediated reversal thermal hyperalgesia CFA model was abolished null but unaltered animals. Hence, due an action on T-type channels, rather than activation

Language: Английский

Voltage-gated Calcium Channels as Potential Therapeutic Targets in Migraine DOI
Juliana Geremias Chichorro, Eder Gambeta, Darciane Favero Baggio

et al.

Journal of Pain, Journal Year: 2024, Volume and Issue: 25(8), P. 104514 - 104514

Published: March 24, 2024

Language: Английский

Citations

2
Contribution of T‐type calcium channel isoforms to cold and mechanical sensitivity in naïve and oxaliplatin‐treated mice of both sexes DOI Creative Commons
Flavia Tasmin Techera Antunes, María A. Gandini, Vinícius M. Gadotti

et al.

British Journal of Pharmacology, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 19, 2024

Background and Purpose The chemotherapy agent oxaliplatin can give rise to oxaliplatin‐induced peripheral neuropathy (OIPN). Here, we investigated whether T‐type calcium channels (Ca v 3) contribute OIPN. Experimental Approach We chronically treated mice with assessed pain responses changes in expression of Ca 3.2 channels. also tested the effects channel blockers on cold sensitivity wild‐type null mice. Key Results Oxaliplatin treatment led mechanical hypersensitivity male female Mechanical persisted both sexes. Intraperitoneal or intrathecal delivery pan inhibitors attenuated but not Remarkably occurred even without treatment. Unexpectedly, intrathecal, intraplantar intraperitoneal Z944 TTA‐P2 transiently induced Acute knockdown specific 3 isoforms revealed that depletion 3.1 males either females triggered hypersensitivity. Finally, reducing by disrupting interactions between deubiquitinase USP5 small organic molecule II‐2 reversed importantly did trigger allodynia. Conclusion Implications Altogether, our data indicate differentially regulation hypersensitivity, raise possibility could promote

Language: Английский

Citations

2
Focusing on C-4 position of Hantzsch 1,4-dihydropyridines: Molecular modifications, enantioseparation, and binding mechanism to L- and T-type calcium channels DOI

Dilara Akman,

Katrin Denzinger, Sun Huang

et al.

European Journal of Medicinal Chemistry, Journal Year: 2022, Volume and Issue: 244, P. 114787 - 114787

Published: Sept. 22, 2022

Language: Английский

Citations

10
Electrophysiological characterization of a CaV3.1 calcium channel mutation linked to trigeminal neuralgia DOI
Abdulaziz M. Alaklabi, Eder Gambeta, Gerald W. Zamponi

et al.

Pflügers Archiv - European Journal of Physiology, Journal Year: 2023, Volume and Issue: 475(6), P. 711 - 718

Published: April 3, 2023

Language: Английский

Citations

5
Analgesia by intrathecal delta-9-tetrahydrocannabinol is dependent on Cav3.2 calcium channels DOI Creative Commons

Vinícius de Maria Gadotti,

Flavia Tasmin Techera Antunes, Gerald W. Zamponi

et al.

Molecular Brain, Journal Year: 2023, Volume and Issue: 16(1)

Published: May 25, 2023

Abstract Delta-9-tetrahydrocannabinol (Δ 9 -THC) is known to produce systemic analgesia that involves CB 1 and 2 cannabinoid receptors. However, there compelling evidence Δ -THC can potently inhibit Cav3.2T-type calcium channels which are highly expressed in dorsal root ganglion neurons the horn of spinal cord. Here, we investigated whether produced by Cav3.2 vis a We show spinally delivered dose-dependent long-lasting mechanical anti-hyperalgesia neuropathic mice, showed potent analgesic effects models inflammatory pain induced formalin or Complete Freund’s Adjuvant (CFA) injection into hind paw, with latter showing no overt sex differences. The mediated reversal thermal hyperalgesia CFA model was abolished null but unaltered animals. Hence, due an action on T-type channels, rather than activation

Language: Английский

Citations

5