Research Progress of circRNAs in Glioblastoma DOI Creative Commons
Xu Guo, Haozhe Piao

Frontiers in Cell and Developmental Biology, Journal Year: 2021, Volume and Issue: 9

Published: Nov. 22, 2021

Circular RNAs (circRNAs) are a class of single-stranded covalently closed non-coding without 5′ cap structure or 3′ terminal poly (A) tail, which expressed in variety tissues and cells with conserved, stable specific characteristics. Glioblastoma (GBM) is the most aggressive lethal tumor central nervous system, characterized by high recurrence mortality rates. The expression circRNAs GBM has demonstrated their potential to become new biomarkers for development GBM. shown as cell proliferation, apoptosis, migration invasion, provides ideas treatment. In this paper, we will review biological properties functions roles clinical applications

Language: Английский

New insight into circRNAs: characterization, strategies, and biomedical applications DOI Creative Commons

Xin-Yi Feng,

Shun-Xin Zhu,

Ke‐Jia Pu

et al.

Experimental Hematology and Oncology, Journal Year: 2023, Volume and Issue: 12(1)

Published: Oct. 12, 2023

Abstract Circular RNAs (circRNAs) are a class of covalently closed, endogenous ncRNAs. Most circRNAs derived from exonic or intronic sequences by precursor RNA back-splicing. Advanced high-throughput sequencing and experimental technologies have enabled the extensive identification characterization circRNAs, such as novel types biogenesis, tissue-specific cell-specific expression patterns, epigenetic regulation, translation potential, localization metabolism. Increasing evidence has revealed that participate in diverse cellular processes, their dysregulation is involved pathogenesis various diseases, particularly cancer. In this review, we systematically discuss databases, challenges for circRNA discovery, new insight into strategies used studies biomedical applications. Although recent advanced understanding knowledge approaches annotation, functional applications continuously needed to provide insights circRNAs. The emergence circRNA-based protein strategy will be promising direction field biomedicine.

Language: Английский

Citations

54
Muscle-derived small extracellular vesicles induce liver fibrosis during overtraining DOI
Ya Liu, Rui Zhou, Yifan Guo

et al.

Cell Metabolism, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

4
RETRACTED ARTICLE: The LINC00623/NAT10 signaling axis promotes pancreatic cancer progression by remodeling ac4C modification of mRNA DOI Creative Commons

Zengyu Feng,

Kexian Li, Kai Qin

et al.

Journal of Hematology & Oncology, Journal Year: 2022, Volume and Issue: 15(1)

Published: Aug. 17, 2022

Abstract Background Although a substantial increase in the survival of patients with other cancers has been observed recent decades, pancreatic ductal adenocarcinoma (PDAC) remains one deadliest diseases. No effective screening approach exists. Methods Differential exosomal long noncoding RNAs (lncRNAs) isolated from serum PDAC and healthy individuals were profiled to screen for potential markers liquid biopsies. The functions LINC00623 cell proliferation, migration invasion confirmed through vivo vitro assays. RNA pulldown, immunoprecipitation (RIP) coimmunoprecipitation (Co-IP) assays rescue experiments performed explore molecular mechanisms LINC00623/NAT10 signaling axis progression. Results A novel lncRNA, LINC00623, was identified, its diagnostic value confirmed, as it could discriminate benign neoplasms individuals. Moreover, shown promote tumorigenicity migratory capacity cells vivo. Mechanistically, bound N-acetyltransferase 10 (NAT10) blocked ubiquitination-dependent degradation by recruiting deubiquitinase USP39. As key regulator N4-acetylcytidine (ac4C) modification mRNA, NAT10 demonstrated maintain stability oncogenic mRNAs their translation efficiency ac4C modification. Conclusions Our data revealed role progression, showing that is biomarker therapeutic target PDAC.

Language: Английский

Citations

60
LINC00941 promotes pancreatic cancer malignancy by interacting with ANXA2 and suppressing NEDD4L-mediated degradation of ANXA2 DOI Creative Commons
Jie Wang, Zhiwei He, Xinyuan Liu

et al.

Cell Death and Disease, Journal Year: 2022, Volume and Issue: 13(8)

Published: Aug. 18, 2022

Abstract Recently, long non-coding RNAs (lncRNA) have been proven to regulate pancreatic cancer (PC) progression. We aimed explore the pathogenesis of LINC00941 in PC regarding protein binding. By using PCR analysis, we found that was overexpressed tissues and higher patients with liver metastasis than without metastasis. In addition, high expression associated a poor prognosis. Functional experiments mice models were respectively used evaluate cell proliferation migration vitro vivo. The results suggested overexpression promoted Subsequently, RNA pull-down, mass spectrometry (MS), RNA-binding immunoprecipitation (RIP) performed identify -interacting proteins. ANXA2 potential protein. Nucleotides 500–1390 could bind Annexin 1 domain ANXA2. -mediated malignant phenotype reversed by depletion. Co-immunoprecipitation (Co-IP) followed MS conducted determine interacting LINC00941. illustrated NEDD4L, an E3 ligase involved ubiquitin-mediated degradation, bound its degradation. Mechanically, functioned as decoy suppressed degradation enclosing binds NEDD4L. Eventually, upregulated activated FAK/AKT signaling, increasing This study indicates promotes binding potentiating stability, leading activation signaling. Our data demonstrate may serve novel target for prognosis therapy.

Language: Английский

Citations

43
A Liquid Biopsy Signature for the Early Detection of Gastric Cancer in Patients DOI
Xin Guo, Yunhua Peng,

Qiying Song

et al.

Gastroenterology, Journal Year: 2023, Volume and Issue: 165(2), P. 402 - 413.e13

Published: March 7, 2023

Language: Английский

Citations

35
Pancreatic Cancer Cell-derived Migrasomes Promote Cancer Progression by Fostering an Immunosuppressive Tumor Microenvironment DOI
Ronghua Zhang,

Junya Peng,

Yalu Zhang

et al.

Cancer Letters, Journal Year: 2024, Volume and Issue: 605, P. 217289 - 217289

Published: Oct. 9, 2024

Language: Английский

Citations

14
MicroRNAs in Pancreatic Cancer: Advances in Biomarker Discovery and Therapeutic Implications DOI Open Access
Roland Madadjim,

Thuy An,

Juan Cui

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(7), P. 3914 - 3914

Published: March 31, 2024

Pancreatic cancer remains a formidable malignancy characterized by high mortality rates, primarily attributable to late-stage diagnosis and dearth of effective therapeutic interventions. The identification reliable biomarkers holds paramount importance in enhancing early detection, prognostic evaluation, targeted treatment modalities. Small non-coding RNAs, particularly microRNAs, have emerged as promising candidates for pancreatic recent years. In this review, we delve into the evolving role cellular circulating miRNAs, including exosomal diagnosis, prognosis, targeting cancer. Drawing upon latest research advancements omics data-driven biomarker discovery, also perform case study using public datasets address commonly identified discrepancies, challenges, limitations. Lastly, discuss analytical approaches that integrate multimodal analyses incorporating clinical molecular features, presenting new insights identifying robust miRNA-centric biomarkers.

Language: Английский

Citations

13
Comprehensive landscape of small non‐coding RNAs in large and small extracellular vesicles from human serum and plasma DOI Creative Commons
Gui‐Yan Xie, Yuxuan Deng, Chunjie Liu

et al.

Deleted Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 7, 2025

Abstract Small non‐coding RNAs (ncRNAs) are functional molecules contained within extracellular vesicles (EVs) that modulate various physiological and pathological processes. This study provides a comprehensive expression profile of seven types small ncRNAs in serum‐ plasma‐derived EVs under conditions. Both large (lEVs) (sEVs) contain high proportions miRNAs (∼28.2% lEVs ∼20.8% sEVs) ribosomal (∼24.0% ∼19.1% sEVs). enriched with more transfer RNA (∼38.8%) than sEVs, whereas sEVs have greater abundance Y (∼22.5%). Notably, is abundant obtained from aged samples (age ≥60 years), pattern not observed lEVs. diverse serum‐derived EVs. There also degree overlap (>50%) the top 100 identified sEVs. The hsa‐miR‐16‐5p, hsa‐let‐7a‐5p, hsa‐miR‐142‐3p, hsa‐miR‐103‐3p consistently among 10 highly expressed plasma‐ as well peripheral blood mononuclear cells. Serum‐derived glioblastoma, breast cancer, prostate gastric cancer specific, miRNAs, snoRNAs, nuclear RNAs, piRNAs. These results elucidate patterns ncRNA cargoes derived serum plasma conditions offer valuable insights for future diagnostic therapeutic applications.

Language: Английский

Citations

1
Small non-coding RNA profiling in patients with non-muscle invasive bladder cancer DOI Creative Commons
Jiajia Cai, Zeqin Yan, Yadi Zhong

et al.

BMC Cancer, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 21, 2025

Language: Английский

Citations

1
Circular RNA circ-MTHFD1L induces HR repair to promote gemcitabine resistance via the miR-615-3p/RPN6 axis in pancreatic ductal adenocarcinoma DOI Creative Commons
Zhi‐Wen Chen, Jianfei Hu,

Zuwei Wang

et al.

Journal of Experimental & Clinical Cancer Research, Journal Year: 2022, Volume and Issue: 41(1)

Published: April 23, 2022

Abstract Background Chemoresistance of pancreatic cancer is the main reason for poor treatment effect patients. Exploring chemotherapy resistance-related genes has been a difficult and hot topic oncology. Numerous studies implicate key roles circular RNAs (circRNAs) in development cancer. However, regulation circRNAs process ductal adenocarcinoma (PDAC) resistance not yet fully clear. Methods Based on cross-analysis Gene Expression Omnibus (GEO) database data our center, we explored new molecule, hsa_circ_0078297 (circ-MTHFD1L), related to resistance. QRT-PCR was used detect expression circRNAs, miRNAs, mRNAs human PDAC tissues their matched normal tissues. The interaction between circ-MTHFD1L miR-615-3p/RPN6 signal axis confirmed by series experiments such as Dual-luciferase reporter assay, fluorescence situ hybridization (FISH) RNA immunoprecipitation (RIP) assays. Results Circ-MTHFD1L significantly increased cells. And patients, higher level circ-MTHFD1L, worse prognosis. Mechanism analysis showed that an endogenous miR-615-3p sponge, upregulates RPN6, thereby promoting DNA damage repair exerting its enhancing gemcitabine More importantly, also found Silencing combined with olaparib can increase sensitivity gemcitabine. Conclusion maintains through axis. may be molecular marker effective PDAC.

Language: Английский

Citations

36