The effect of copper acetate on hepatocyte metabolism in vitro DOI Creative Commons
I. S. Trukhan, Н. Н. Дремина, И. А. Шурыгина

et al.

Acta Biomedica Scientifica (East Siberian Biomedical Journal), Journal Year: 2024, Volume and Issue: 9(6), P. 12 - 21

Published: Dec. 28, 2024

Background . Copper ions are necessary for maintaining basic physiological processes in the mammalian organism. However, their excessive absorption or accumulation cells can lead to development exacerbation of various pathological processes. The cytotoxic and genotoxic effects high concentrations copper compounds currently well studied cell cultures, whereas effect non-toxic amounts on cells, including during cultivation, has been extremely poorly studied. aim study To investigate changes intracellular amount mitochondrial cytochrome C oxidase glutathione synthetase. Materials methods A primary culture hepatocytes was obtained, which exposed acetate at a concentration 200 µg/ml terms content 24 hours. After fixation, samples were stained immunocytochemically using antibodies (CcO) subunit I synthetase (GS). Results In hepatocyte culture, significant increase intensity fluorescent staining two analyzed enzymes demonstrated both after 6 hours exposure ions, indicates change number cells. At same time, CcO more intense first incubation with microelement, next 18 hours, less pronounced. GS stain active observed throughout entire cultivation period. Conclusion From results it be concluded that able influence key indicators viability by changing one main energy metabolism enzyme provides synthesis most important low-molecular antioxidant glutathione.

Language: Английский

Metal-organic coordinated self-assembled nanomedicine for enhanced cuproptosis-mediated chemo-photo-chemodynamic synergistic therapy of non-small cell lung cancer DOI
R.P. Liu, Ruirui Zhao,

Zi-Wei Yu

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 161305 - 161305

Published: March 1, 2025

Language: Английский

Citations

1
The Regulation of Trace Metal Elements in Cancer Ferroptosis DOI Creative Commons

Xiaoyan Wang,

Yuanyuan Xue, Lei Chang

et al.

Advanced Biology, Journal Year: 2025, Volume and Issue: unknown

Published: April 9, 2025

Abstract Ferroptosis, as novel type of regulated cell death that has garnered widespread attention over the past decade, witnessed continuous discovery an increasing number regulatory mechanisms. Trace metal elements play a multifaceted and crucial role in oncology. Interestingly, it been increasingly evident these elements, such copper, are involved regulation iron accumulation, lipid peroxidation antiferroptotic systems, suggesting existence “nonferrous” mechanisms ferroptosis. In this review, comprehensive overview composition mechanism ferroptosis is provided. The interaction between copper metabolism (including cuproptosis) cancer, well roles other trace (such zinc, manganese, cobalt, molybdenum) specifically focused. Furthermore, applications nanomaterials based on metals cancer therapy also reviewed potential strategies for co‐targeting cuproptosis explored. Nevertheless, light intricate ambiguous nature interactions, ongoing research essential to further elucidate ferroptosis, thereby facilitating development therapeutic targets approaches treatment.

Language: Английский

Citations

1
Targeting pyroptosis for cancer immunotherapy: mechanistic insights and clinical perspectives DOI Creative Commons
Chen Huang, Jiayi Li, Ruiyan Wu

et al.

Molecular Cancer, Journal Year: 2025, Volume and Issue: 24(1)

Published: May 3, 2025

Pyroptosis is a distinct form of programmed cell death characterized by the rupture membrane and robust inflammatory responses. Increasing evidence suggests that pyroptosis significantly affects tumor microenvironment antitumor immunity releasing damage-associated molecular patterns (DAMPs) pro-inflammatory mediators, thereby establishing it as pivotal target in cancer immunotherapy. This review thoroughly explores mechanisms underlying pyroptosis, with particular focus on inflammasome activation gasdermin family proteins (GSDMs). It examines role pyroptotic reshaping immune (TIME) involving both cells, discusses recent advancements targeting pathways through therapeutic strategies such small molecule modulators, engineered nanocarriers, combinatory treatments checkpoint inhibitors. We also advances future directions to enhance immunotherapy inhibitors, adoptive therapy, vaccines. study suggested offers promising avenue amplify responses surmount resistance existing immunotherapies, potentially leading more efficacious treatments.

Language: Английский

Citations

1
Targeting the initiator to activate both ferroptosis and cuproptosis for breast cancer treatment: progress and possibility for clinical application DOI Creative Commons

Murshid Imam,

Jiale Ji,

Zhijie Zhang

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 10, 2025

Breast cancer is the most commonly diagnosed worldwide. Metal metabolism pivotal for regulating cell fate and drug sensitivity in breast cancer. Iron copper are essential metal ions critical maintaining cellular function. The accumulation of iron triggers distinct death pathways, known as ferroptosis cuproptosis, respectively. Ferroptosis characterized by iron-dependent lipid peroxidation, while cuproptosis involves copper-induced oxidative stress. They increasingly recognized promising targets development anticancer drugs. Recently, compelling evidence demonstrated that interplay between plays a crucial role progression. This review elucidates converging pathways Moreover, we examined value genes associated with clinical diagnosis treatment cancer, mainly outlining potential co-targeting approach. Lastly, delve into current challenges limitations this strategy. In general, offers an overview interaction offering valuable perspectives further research treatment.

Language: Английский

Citations

0
Identification and Immunological Characterization of Cuproptosis Related Genes in Preeclampsia Using Bioinformatics Analysis and Machine Learning DOI Creative Commons
Tiantian Yu, Guiying Wang,

Xia Xu

et al.

Journal of Clinical Hypertension, Journal Year: 2025, Volume and Issue: 27(1)

Published: Jan. 1, 2025

Preeclampsia (PE) is a pregnancy-specific disorder characterized by an unclearly understood pathogenesis and poses great threat to maternal fetal safety. Cuproptosis, novel form of cellular death, has been implicated in the advancement various diseases. However, role cuproptosis immune-related genes PE unclear. The current study aims elucidate gene expression matrix immune infiltration patterns cuproptosis-related (CRGs) context PE. GSE98224 dataset was obtained from Gene Expression Omnibus (GEO) database utilized as internal training set. Based on dataset, we explored differentially expressed related (DECRGs) immunological composition. We identified 10 DECRGs conducted Ontology (GO) function, Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment analyses, protein-protein interaction (PPI) network. Furthermore, patients with were categorized into two distinct clusters, investigation examine status cell infiltration. Additionally, application Weighted Co-expression Network Analysis (WGCNA) differentiate modules consisting co-expressed conduct clustering analysis. intersecting differently clusters. most precise forecasting model chosen evaluating effectiveness four machine learning models. ResNet established score hub genes. prediction accuracy assessed receiver operating characteristic (ROC) curves external dataset. successfully five key DECREGs pathological clusters PE, each profiles biological characteristics. Subsequently, RF deemed optimal for identification large area under curve (AUC = 0.733). that ranked highest considered be predictor calibration demonstrated high level aligning predicted outcomes actual outcomes. validate using ROC 0.82). Cuproptosis may play important present elucidated GSTA4, KCNK5, APLNR, IKZF2, CAP2 potential markers cuproptosis-associated are significant initiation development cuproptosis-induced

Language: Английский

Citations

0
Cuproptosis Nanoprodrug-Initiated Self-Promoted Cascade Reactions for Postoperative Tumor Therapy DOI
Ruirui Yan,

Xuan Cheng,

Yujing Song

et al.

Biomaterials, Journal Year: 2025, Volume and Issue: 318, P. 123176 - 123176

Published: Feb. 7, 2025

Language: Английский

Citations

0
A prognostic model for lung adenocarcinoma based on cuproptosis and disulfidptosis related genes revealing the key prognostic role of FURIN DOI Creative Commons
Ji Young You,

Qing Yu,

Ronghui Chen

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 19, 2025

Lung adenocarcinoma (LUAD) is the most common subtype of lung cancer. Despite advances in treatment, prognosis remains poor due to late diagnosis. Cuproptosis (driven by copper ion accumulation) and disulfidptosis disulfide bond are novel forms programmed cell death, closely linked tumor initiation, progression, resistance. However, specific roles these mechanisms LUAD remain inadequately studied. This study integrated multi-omics data from TCGA GEO databases systematically evaluate differential expression prognostic significance disulfide-related genes (DCRGs), identify two DCRG molecular subtypes, construct a scoring model based on four key genes. Multi-omics analysis results revealed that score not only accurately predicts patients but also associated with immune infiltration patterns EGFR inhibitor responses. RT-qPCR validated high FURIN RHOV cells, supporting their role as potential therapeutic targets. Further Mendelian randomization confirmed causal relationship between development. These findings provide biomarkers for evaluation cuproptosis offer theoretical basis targeting treatment.

Language: Английский

Citations

0
Strategies to combat cancer drug resistance: focus on copper metabolism and cuproptosis DOI Open Access

Leyi Yao,

Baoyi Jiang,

Dacai Xu

et al.

Cancer Drug Resistance, Journal Year: 2025, Volume and Issue: unknown

Published: March 26, 2025

Cancer cells often develop tolerance to chemotherapy, targeted therapy, and immunotherapy drugs either before or during treatment. The significant heterogeneity among various tumors poses a critical challenge in modern cancer research, particularly overcoming drug resistance. Copper, as an essential trace element the body, participates biological processes of diseases, including cancers. growth many types tumor exhibits heightened dependence on copper. Thus, targeting copper metabolism inducing cuproptosis may be potential ways overcome Copper chelators have shown resistance by copper-dependent cells. In contrast, ionophores, copper-based nanomaterials, other small molecules been used induce cell death (cuproptosis) cells, drug-resistant This review summarizes regulation role resistance, providing ideas for future.

Language: Английский

Citations

0
Integrating cuproptosis and immunosenescence: A novel therapeutic strategy in cancer treatment DOI
Ali Ahmadizad Firouzjaei, Seyed Hamid Aghaee‐Bakhtiari

Biochemistry and Biophysics Reports, Journal Year: 2025, Volume and Issue: 42, P. 101983 - 101983

Published: March 29, 2025

Language: Английский

Citations

0
An up‐To‐Date Review of Elesclomol and Its Nano‐Formulations in Cancer Therapy DOI Creative Commons
Qi Wang, Feng Li, Amit K. Tiwari

et al.

Cancer Reports, Journal Year: 2025, Volume and Issue: 8(4)

Published: April 1, 2025

ABSTRACT Background Elesclomol (ES) is a promising anticancer compound that exerts its effects through multiple mechanisms. It acts as copper (Cu(II)) ionophore, forming an ES–Cu complex within cancer cells and inducing novel form of cell death called cuproptosis. Aim To provide up‐to‐date review on elesclomol nano‐formulations with particular focus therapy. Sources Literature was collected by manually searching in Pubmed, Google Scholar, clinicaltrials.gov March 2025. Content This provides overview the discovery development ES molecule, including physicochemical properties. New insights into intracellular interactions mechanisms transportation are then explained. The recent clinical outcomes therapy, both monotherapy combination paclitaxel or carboplatin, summarized. While initial trials showed promise, more studies focusing preclinical investigations complex. Nanomedicine‐based formulations have emerged strategy to enhance delivery well therapeutic effects, several nanomedicines currently under development. nanoparticle strategies discussed. comprehensive advancements study, mechanism action, progress, potential nanomedicine‐based approaches improve efficacy treatment.

Language: Английский

Citations

0