CAR-NK cells: harnessing the power of natural killers for advanced cancer therapy DOI Creative Commons
Filipa D. dos Reis,

Yanis Saidani,

Paula Martín-Rubio

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: May 30, 2025

Generation of Chimeric Antigen Receptors (CARs) presented a significant advance in the field immunotherapy, allowing targeting cell-surface expressed molecules an MHC-independent manner. Arming NK cells with CARs merges their innate natural cytotoxicity refined precision targeted antigen recognition. The success these therapies hinges on selecting right tumor-specific targets to ensure effective activation and avoid self-reactivity. Optimization CAR design is based cell intrinsic properties (CAR modules sources cells), as well NK-tumor interactions (multi-antigen, multi-step, multi-switch). Additionally, dynamics tumor infiltration adaptation microenvironment play critical role CAR-NK efficacy. Combining chemotherapy, radiotherapy, checkpoint inhibitors, emerging approaches like epigenetic modulators oncolytic viruses, may address some challenges. development strategies for metastatic disease especially promising, though complexities metastasis require designs. As immunomics multi-omics continue evolve, potential designing more expands. results from preclinical clinical trials unfold, multidisciplinary approach integrating all those aspects will be key unlock full cell-based adoptive transfers.

Language: Английский

Bacterial-Mediated In Situ Engineering of Tumour-Associated Macrophages for Cancer Immunotherapy DOI Open Access

Gabriela Christina Kuhl,

Mark Tangney

Cancers, Journal Year: 2025, Volume and Issue: 17(5), P. 723 - 723

Published: Feb. 20, 2025

Background/Objectives: Tumour-associated macrophages (TAMs) are critical components of the tumour microenvironment (TME), significantly influencing cancer progression and treatment resistance. This review aims to explore innovative use engineered bacteria reprogram TAMs, enhancing their anti-tumour functions improving therapeutic outcomes. Methods: We conducted a systematic following predefined protocol. Multiple databases were searched identify relevant studies on phenotypic plasticity, for reprogramming. Inclusion exclusion criteria applied select studies, data extracted using standardised forms. Data synthesis was performed summarise findings, focusing mechanisms benefits non-pathogenic modify TAMs. Results: The summarises findings that can selectively target promoting shift from tumour-promoting M2 phenotype tumour-fighting M1 phenotype. reprogramming enhances pro-inflammatory responses activity within TME. Evidence various indicates significant regression improved immune bacterial therapy. Conclusions: Reprogramming TAMs presents promising strategy approach leverages natural targeting abilities directly tumour, potentially patient outcomes offering new insights into immune-based treatments. Further research is needed optimise these methods assess clinical applicability.

Language: Английский

Citations

1
Global research trends of immunosenescence and immunotherapy: A bibliometric study DOI Creative Commons
Wen‐Di Li, Lin Xiao, Haiyang Li

et al.

Human Vaccines & Immunotherapeutics, Journal Year: 2025, Volume and Issue: 21(1)

Published: Feb. 24, 2025

Immunosenescence refers to the gradual decline in immune system function with age, increasing susceptibility infections and cancer elderly. The advent of novel immunotherapies has revolutionized field treatment. However, majority patients exhibit poor re-sponses immunotherapy, immunosenescence likely playing a significant role. In recent years, progress been made understanding interplay between immunotherapy. Our research aims explore prospects development trends immunotherapy using bibliometric analysis. Relevant articles were collected from Web Science Core Collection (WoSCC) (retrieved on July 20, 2024). Primary characteristics analyzed R package "Biblio-metrix," keyword co-occurrence analysis visualization conducted VOSviewer. A total 213 English-language original review spanning 35 years re-trieved for There was surge publications this starting 2017. United States China contributed most articles. Frontiers Immunology productive journal, while University California System highest contributing institution. Besse Benjamin France emerged as influential researcher field. Popular keywords included "nivolumab," "T cells," "dendritic "regulatory T cells." "immunosenescence-associated secretory phenotype" become new hotspot, checkpoint inhibitors remaining central theme domain. is entering phase rapid will continue hold value future research.

Language: Английский

Citations

1
Artificial intelligence-based radiogenomics reveals the potential immunoregulatory role of COL22A1 in glioma and its induced autoimmune encephalitis DOI Creative Commons

Bingchao Yan,

Qian Chen, Da‐Cheng Wang

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: March 6, 2025

Background The tumor microenvironment plays a crucial role in the progression of both glioma and glioma-induced autoimmune encephalitis. However, there remains significant lack effective therapeutic targets for these diseases. Method We collected 54 CT images patients encephalitis patients, respectively. Radiomics features were extracted from tumors regions using Python, followed by dimensionality reduction via random forest lasso regression, construction radiomics-based risk scores. Genomic data matched with clinical information analyzed to identify key prognostic genes significantly associated Gene expression was validated immunohistochemistry our samples. Immune infiltration evaluated five algorithms (MCP-counter, EPIC, TIMER, QUANT IPS). association between hub immune checkpoint markers as well immunoregulation-related also Spearman correlation. Results identified 980 radiomics patient selected four through regression build score. COL22A1 strongly correlated score gene. higher glioblastoma tissues cell lines, factors such age, WHO grade, IDH mutation status. analysis indicated associations diverse stromal populations, including CD8 + T cells, macrophages, CAFs. positively checkpoints immune-regulated genes. Conclusion Our study highlights critical gliomas glioma-Induced Autoimmune Encephalitis, demonstrating its strong poor prognosis involvement regulation.

Language: Английский

Citations

1
Organoid models of ovarian cancer: resolving immune mechanisms of metabolic reprogramming and drug resistance DOI Creative Commons
Lanyue Zhang,

Jiangnan Zhao,

Changqing Su

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: March 21, 2025

Metabolic reprogramming is a hallmark of ovarian cancer, enabling tumor progression, immune evasion and drug resistance. The microenvironment (TME) further shapes metabolic adaptations, cancer cells to withstand hypoxia nutrient deprivation. While organoid models provide physiologically relevant platform for studying these processes, they still lack vascular components, limiting their ability fully recapitulate metabolism responses. In this study, we investigated the key mechanisms involved in focusing on glycolysis, lipid amino acid metabolism. We integrated metabolomic analyses sensitivity assays explore metabolic-TME interactions using patient-derived, adult stem cell-derived iPSC-derived organ tissues. Among these, found that play central role progression chemotherapy identified methylglyoxal (MGO)-mediated BRCA2 dysfunction as driver escape, sphingolipid signaling proliferation kynurenine CD8+ T cell suppression. addition, PI3K/AKT/mTOR Wnt/β-catenin pathways promote chemoresistance through adaptation. By elucidating link between evasion, study identifies vulnerabilities potential targets cancer. Our findings support development metabolically targeted therapies increase utility organoid-based precision medicine models.

Language: Английский

Citations

1
Management of metastatic melanoma with combinations including PD-1 inhibitors DOI Creative Commons
Lara Valeska Maul, Egle Ramelyte,

Reinhard Dummer

et al.

Expert Opinion on Biological Therapy, Journal Year: 2025, Volume and Issue: unknown

Published: March 30, 2025

Introduction Melanoma is among the most immunogenic malignancies. The advent of immune checkpoint inhibitors (ICIs) has revolutionized landscape melanoma treatment. Long-term durable cancer control possible in nearly 50% non-resectable, metastatic patients with anti-CTLA4 and anti-PD-1 antibodies.

Language: Английский

Citations

1
Serine metabolism in tumor progression and immunotherapy DOI Creative Commons
Dong Huang, Hui Cai,

Hazel Huang

et al.

Discover Oncology, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 28, 2025

Language: Английский

Citations

1
Unveiling purine metabolism dysregulation orchestrated immunosuppression in advanced pancreatic cancer and concentrating on the central role of NT5E DOI Creative Commons
Junqian Zhang, Xiaobo Zhang, R. Wu

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: April 1, 2025

The dismal efficacy of immunotherapy for Pancreatic cancer (PC) can be predominantly ascribed to its distinctive cold-tumor properties. by-products purine metabolic reprogramming are extensively engaged in tumor immune modulation, influencing the functions and recruitment cells molding an microenvironment that is propitious growth. We harnessed single-cell transcriptomics spatial concurrently analyze metabolism (PM) features PC microenvironment. quantitatively appraised PM traits diverse cell subsets via scoring algorithms such as AUCell Ucell. Moreover, development cell-cell interaction analysis elucidated alterations TME induced by dysregulation. Additionally, we defined disorder characteristics patients utilized this assess phenotypes prognoses patient population. Also, identified crucial intermediate genes impact establishment immunosuppressive environment within PC, validated them through sectioning co-culture experiments. Multi - dimensional transcriptome data unique heterogeneity microenvironment, which manifested fibroblasts demonstrating higher scores TME. Cellchat revealed malignant with elevated expression were concomitantly associated frequent interactions CAFs well high ligand-receptor pairs transcription factors. Spatial further corroborated finding. Furthermore, newly constructed criteria indicated levels a lack response Finally, study singular role NT5E immunosuppression resulting from PC. CCK8 invasion experiments following model demonstrated intervention targeting could reverse augmented malignancy co-cultured CAFs. potentially key target reversing "stiff-cancer" This demonstrates disorders impinge upon exacerbate engendered progression fibrosis. Therapeutic strategies or may offer ray hope advanced PDAC.

Language: Английский

Citations

0
RNA modifications in the tumor microenvironment: insights into the cancer-immunity cycle and beyond DOI Creative Commons
Yuan Ding, Cuicui Liu, Ke‐Da Yu

et al.

Experimental Hematology and Oncology, Journal Year: 2025, Volume and Issue: 14(1)

Published: April 2, 2025

Language: Английский

Citations

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The role of cancer‐associated fibroblasts in the tumour microenvironment of urinary system DOI Creative Commons

Ri Hong,

Puguang Yu,

Xiaoli Zhang

et al.

Clinical and Translational Medicine, Journal Year: 2025, Volume and Issue: 15(4)

Published: April 1, 2025

Abstract Urological tumours are a type of neoplasms that significantly jeopardise human life and wellbeing. Cancer‐associated fibroblasts (CAFs), serving as the primary component stromal cellular milieu, form diverse cohort exerts substantial influence on tumourigenesis tumour progression. In this review, we summarised literatures regarding functions CAFs in urinary microenvironment (TME). We primarily examined multifaceted activities TME urological system tumours, including inhibiting immunity, remodelling extracellular matrix, promoting growth, metastasis, drug resistance their clinical applications. also discussed potential future directions for leveraging artificial intelligence research. Key points The interaction with various cell secretory factors tumors. application diagnosis, treatment prognosis

Language: Английский

Citations

0
Investigation of c-Fos/c-Jun Signaling Pathways in Periostracum Cicadae’s Inhibition of EMT in Gastric Tissue DOI Creative Commons
Hua Liang,

Xiaofei Jin,

Tao He

et al.

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(4), P. 537 - 537

Published: April 7, 2025

Background/Objectives: Periostracum Cicadae (PC) is commonly used to treat chronic atrophic gastritis (CAG), but its underlying mechanisms are unclear. We investigated the therapeutic effects, active ingredients and molecular of PC on CAG. Methods: analyzed components in serum extract by UHPLC-Q-Orbitrap-MS/MS. Then, we rat cell models assess impact CAG employed network pharmacology bioinformatics predict key targets ingredients. Finally, confirmed hub through experiments docking. Results: A total 22 were identified extract-containing using UHPLC-Q-Orbitrap MS/MS. Network combined with docking revealed that protective effect was primarily mediated three compounds: (Z)-akuammidine, chicoric acid, columbianadin. And c-Fos/c-Jun signaling pathways crucial therapy. inhibited vitality, migration, invasion, multiplication MC cells (model for CAG), induced apoptosis, caused G0/G1 phase cycle arrest. The expression level tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), interleukin-1 beta (IL-1β) gastrin 17 (G17) rats increased, while pepsinogen I (PG I) II II) decreased. After 12 weeks administration, these conditions significantly improved. not only reduced levels antigen KI-67 (Ki67) protein p53 (P53) also enhanced SRY-box Transcription Factor (SOX2). Simultaneously, down-regulated N-cadherin Vimentin up-regulating E-cadherin. Conclusions: epithelial–mesenchymal transition (EMT) via pathway, thereby providing benefits Our study elucidates material basis treating CAG, experimental evidence support clinical application.

Language: Английский

Citations

0