The impact of androgen-induced translation in modulating androgen receptor activity DOI Creative Commons

Justus Simon Israel,

Laura-Maria Marcelin,

Sherif Mehralivand

et al.

Biology Direct, Journal Year: 2024, Volume and Issue: 19(1)

Published: Nov. 11, 2024

Dysregulated androgen receptor (AR) activity is central to various diseases, particularly prostate cancer (PCa), in which it drives tumour initiation and progression. Consequently, antagonising AR via anti-androgens an indispensable treatment option for metastatic PCa. However, despite initial remission, drug resistance occurs. Therefore, the signalling pathway has been intensively investigated. role of protein stability therapy not yet deciphered. this study aimed investigate changes transactivity assess its mechanism as a possible target LNCaP, C4-2, 22Rv1 cells were treated with R1881, enzalutamide, cycloheximide, Rocaglamide. Mass spectrometry analyses performed on LNCaP identify pathways enriched by treatments. Western blotting was levels localisation changes. Changes determined qPCR. decipher molecular mechanisms underlying androgen- antiandrogen-induced alterations protein. Pathway analysis revealed enrichment proteins involved different that regulate translation. Translational proteasome inhibitor experiments these attributable modifications translational activity. Interestingly, effects castration-resistant PCa (CRPC) C4-2 or enzalutamide-resistant less prominent non-existent. This outcome similarly observed alteration transactivation, suppressed hormone-sensitive (HSPC) inhibition, akin effect enzalutamide. In contrast, treatment-resistant cell lines showed only slight change transcription. suggests HSPC, activation triggers cascade increases enhancing translation rate, thereby amplifying appears be dysregulated cells.

Language: Английский

Innovations and Emerging Trends in Prostate Cancer Management: A Literature Review DOI Open Access

Nazeer Ibraheem,

Momen Abdelglil,

Andrew Wanees

et al.

Cureus, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 6, 2024

Prostate cancer (PC) is considered the second most diagnosed in men worldwide. It remains a leading cause of cancer-related death. Recently, many modalities have been discovered and used diagnosis management PC, with incorporation treatment options such as hormonal therapy, chemotherapy, targeted therapies, immunotherapy, precision medicine. Robotics artificial intelligence (AI) further modified PCs, improving accuracy disease progression. This comprehensive review offers an in-depth exploration historical treatments, evaluation current therapeutic techniques, discussion use robotic surgery AI, examination ongoing clinical trials emerging procedures. Additionally, this also covers challenges. By inspecting these aspects, may provide valuable information regarding future research practice directions PC treatment, contributing to thorough understanding complex context management.

Language: Английский

Citations

0
The impact of androgen-induced translation in modulating androgen receptor activity DOI Creative Commons

Justus Simon Israel,

Laura-Maria Marcelin,

Sherif Mehralivand

et al.

Biology Direct, Journal Year: 2024, Volume and Issue: 19(1)

Published: Nov. 11, 2024

Dysregulated androgen receptor (AR) activity is central to various diseases, particularly prostate cancer (PCa), in which it drives tumour initiation and progression. Consequently, antagonising AR via anti-androgens an indispensable treatment option for metastatic PCa. However, despite initial remission, drug resistance occurs. Therefore, the signalling pathway has been intensively investigated. role of protein stability therapy not yet deciphered. this study aimed investigate changes transactivity assess its mechanism as a possible target LNCaP, C4-2, 22Rv1 cells were treated with R1881, enzalutamide, cycloheximide, Rocaglamide. Mass spectrometry analyses performed on LNCaP identify pathways enriched by treatments. Western blotting was levels localisation changes. Changes determined qPCR. decipher molecular mechanisms underlying androgen- antiandrogen-induced alterations protein. Pathway analysis revealed enrichment proteins involved different that regulate translation. Translational proteasome inhibitor experiments these attributable modifications translational activity. Interestingly, effects castration-resistant PCa (CRPC) C4-2 or enzalutamide-resistant less prominent non-existent. This outcome similarly observed alteration transactivation, suppressed hormone-sensitive (HSPC) inhibition, akin effect enzalutamide. In contrast, treatment-resistant cell lines showed only slight change transcription. suggests HSPC, activation triggers cascade increases enhancing translation rate, thereby amplifying appears be dysregulated cells.

Language: Английский

Citations

0