Exosome-transmitted LUCAT1 promotes stemness transformation and chemoresistance in bladder cancer by binding to IGF2BP2 DOI Creative Commons
Yonghao Zhan,

Zhenzhen Zhou,

Zhaowei Zhu

et al.

Journal of Experimental & Clinical Cancer Research, Journal Year: 2025, Volume and Issue: 44(1)

Published: March 3, 2025

Abstract The chemotherapy resistance is an awkward challenge in management of bladder cancer (BC). Cancer organoid model effective preclinical tumor that could faithfully represent clinical manifestations and simulate the biological processes chemoresistance. Recent studies have revealed stem cells (CSCs) play a significant role development chemoresistance cancer. Exosomes act as essential intercellular messengers participate controlling conversion distinct cell characteristics, including However, exosome-transmitted lncRNAs has rarely been reported. In this study, models were developed from urothelial carcinomas to explore pathophysiology mechanism BC chemoresistance, RNA-seq was performed screen for involved BC. We found enriches stem-like BC, upregulation Lung Associated Transcript 1 (LUCAT1) occurs chemotherapy-resistant organoids correlated with response. Further experimental results demonstrated LUCAT1 promotes by enhancing stemness phenotype vivo vitro. Moreover, exosomes derived can enhance delivering LUCAT1. Mechanistically, significantly mRNA stability HMGA1 via binding IGF2BP2 m6A-dependent manner. study demonstrates important potential function both diagnostic biomarker therapeutic target

Language: Английский

Exosome-transmitted LUCAT1 promotes stemness transformation and chemoresistance in bladder cancer by binding to IGF2BP2 DOI Creative Commons
Yonghao Zhan,

Zhenzhen Zhou,

Zhaowei Zhu

et al.

Journal of Experimental & Clinical Cancer Research, Journal Year: 2025, Volume and Issue: 44(1)

Published: March 3, 2025

Abstract The chemotherapy resistance is an awkward challenge in management of bladder cancer (BC). Cancer organoid model effective preclinical tumor that could faithfully represent clinical manifestations and simulate the biological processes chemoresistance. Recent studies have revealed stem cells (CSCs) play a significant role development chemoresistance cancer. Exosomes act as essential intercellular messengers participate controlling conversion distinct cell characteristics, including However, exosome-transmitted lncRNAs has rarely been reported. In this study, models were developed from urothelial carcinomas to explore pathophysiology mechanism BC chemoresistance, RNA-seq was performed screen for involved BC. We found enriches stem-like BC, upregulation Lung Associated Transcript 1 (LUCAT1) occurs chemotherapy-resistant organoids correlated with response. Further experimental results demonstrated LUCAT1 promotes by enhancing stemness phenotype vivo vitro. Moreover, exosomes derived can enhance delivering LUCAT1. Mechanistically, significantly mRNA stability HMGA1 via binding IGF2BP2 m6A-dependent manner. study demonstrates important potential function both diagnostic biomarker therapeutic target

Language: Английский

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