Translocase of Outer Mitochondrial Membrane 40, as a Promising Biomarker for the Diagnosis of Polycystic Ovary Syndrome and Pan-Cancer DOI Creative Commons
Xin Zhang, Lin Zhu,

ChenHao Ma

et al.

Reproductive Sciences, Journal Year: 2024, Volume and Issue: 31(11), P. 3569 - 3582

Published: July 26, 2024

Polycystic ovary syndrome (PCOS) is a metabolic disease that affects the reproductive system, and its pathogenesis remains unresolved. Through application of bioinformatics molecular biology techniques, this study has identified significant association between translocase outer mitochondrial membrane 40 (TOMM40) both PCOS pan-cancers. The selection biomarkers included TOMM40, which we found to be significantly decreased in group vitro vivo, using methods such as Western Blot well immunohistochemistry. Over-expression TOMM40 can rescue effect on apoptosis rate proliferation suppression induced by DHEA KGN cells. biomarker for diagnosis PCOS. pan-cancer analysis revealed an elevated expression Uterine Corpus Endometrial Carcinoma unfavorable prognosis, while increased six tumor types was linked favorable prognosis. Therefore, regarded promising diagnosing pan-cancer.

Language: Английский

Woxuanzhongzhou formula improves DHEAS and high-fat diet-induced IR and anovulatory mice via AMPK/PGC1- α/Irisin pathway DOI Creative Commons

Haijuan Liu,

Guohua Wang,

Conglu Sui

et al.

Journal of Ovarian Research, Journal Year: 2025, Volume and Issue: 18(1)

Published: Jan. 16, 2025

Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder in women of reproductive age. Anovulation one the most important clinical features PCOS, insulin resistance (IR) critical pathogenic factors. Woxuanzhongzhou (WXZZ) traditional herbal formulation that has shown efficacy treating PCOS combined with IR, but underlying mechanism not clear. The aim this study was to investigate molecular WXZZ on dehydroepiandrosterone sulfate high fat diet induced IR mice. 40 female C57BL/6 mice were randomized 4 groups: control group, model metformin group. Some by (DHEA) high-fat (HFD) for 3 weeks. Following induction, administered gavage. Body weight, fasting blood glucose (FBG), (FINS) levels, homeostatic assessment (HOMA-IR), gonadal hormones measured. Estrous cycles monitored. structure gastrocnemius muscle subcutaneous fatty tissue also evaluated. Additionally, serum irisin non-esterified acids (NEAF) levels protein gene expression AMPK, PGC1-α, FNDC5, CaMKK, UCP1 analyzed. DHEA + HFD group exhibited significant improvements several key parameters compared ameliorated disorders, resumed estrous cycle DHEAS diet-induced anovulatory Significant reductions observed body testosterone, luteinizing hormone, hormone/ follicle-stimulating hormone ratio, FINS, HOMA-IR. promoted secretion up-regulating AMPK/PGC1-α/FNDC5 up-regulated CaMKK/AMPK/PGC1-α/UCP1 fat. inhibited overproduction NEFA, reduced lipid accumulation. Structural analysis adipose revealed partial restoration. exhibits therapeutic effects These may be mediated through activation AMPK/PGC1-α pathway promote irisin.

Language: Английский

Citations

1
Extracellular Vesicles from Adipose‐Derived Mesenchymal Stem Cells Combined with PEG Hydrogel Alleviate Maternal Simulated Birth Injury in a Rat Model DOI
Qīng Wáng, Yaqin Li, Yue Zhang

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

Abstract Pelvic organ prolapse (POP) is a common and distressing condition affecting women, particularly those with history of vaginal delivery. The impact extracellular vesicles derived from adipose‐derived mesenchymal stem cells (ADSC‐EVs) on pelvic floor tissue injury remains unclear. Due to their short half‐life rapid clearance in vivo, ADSC‐EVs lose efficacy quickly. To address this, an injectable tetra‐PEG hydrogel encapsulate (PEG@EVs) developed. formed by tetra‐PEG‐NH 2 tetra‐PEG‐NHS through ammonolysis reaction, leading the formation amide bonds within seconds. Vaginal wall POP patients shows disruption matrix, lipid peroxidation, inflammation. In vitro, significantly reduce H₂O₂‐induced oxidative stress, oxidation, apoptosis, while enhancing expression Nrf2 its downstream targets—CAT, NQO1, HO‐1, SOD2. also upregulate GPX4 SLC7A11, reducing mitochondrial damage mitigating ferroptosis. inhibitor ML385 reverses these protective effects. rat model childbirth injury, PEG@EVs treatment promotes nuclear translocation, induces M1‐to‐M2 macrophage conversion, reduces inflammation, stimulates collagen deposition, thereby accelerating repair. findings this study may serve as foundation for early targeted intervention POP, representing promising therapeutic approach.

Language: Английский

Citations

0
Melatonin refines ovarian mitochondrial dysfunction in PCOS by regulating the circadian rhythm gene Clock DOI Creative Commons
Wenxiu Chen,

Hongyan Zhang,

Bao Guo

et al.

Cellular and Molecular Life Sciences, Journal Year: 2025, Volume and Issue: 82(1)

Published: March 6, 2025

Mitochondrial dysfunction is present in the ovaries of patients with polycystic ovary syndrome (PCOS). Melatonin (MT) has shown promise treating PCOS by improving mitochondrial dysfunction, though underlying mechanisms remain unclear. In this study, we first assessed levels proteins associated autophagy and dynamics granulosa cells (GCs) DHEA-induced mice. We found abnormal expression these proteins, indicating presence ovaries. Notably, circadian gene Clock melatonin synthetic enzymes were also decreased patients. Studies have suggested a potential role rhythm genes pathogenesis progression PCOS. subsequently observed that pretreatment MT could ameliorate mitochondrial-related reverse low CLOCK, reduce pyroptosis Given interaction between Clock, focused on whether exogenous improves regulating Clock. Through vitro culture human ovarian cell line KGN, further when CLOCK inhibited, beneficial effects autophagy, disturbed dynamics, not significant, there was no notable improvement GCs pyroptosis. Our study suggests may improve while reducing

Language: Английский

Citations

0
Human placental mesenchymal stromal cells alleviate intestinal oxidative damage in mice with graft-versus-host disease via CD73/adenosine/PI3K/Akt/GSK-3β axis DOI
Kaiyue Han, Feifei Wang, Xiaolin Ma

et al.

Cellular Signalling, Journal Year: 2024, Volume and Issue: 123, P. 111372 - 111372

Published: Aug. 28, 2024

Language: Английский

Citations

3
Mitochondria in skeletal system-related diseases DOI Open Access

Liang Pei,

Zizhen Yao, Liang Dong

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 181, P. 117505 - 117505

Published: Nov. 4, 2024

Language: Английский

Citations

1
Translocase of Outer Mitochondrial Membrane 40, as a Promising Biomarker for the Diagnosis of Polycystic Ovary Syndrome and Pan-Cancer DOI Creative Commons
Xin Zhang, Lin Zhu,

ChenHao Ma

et al.

Reproductive Sciences, Journal Year: 2024, Volume and Issue: 31(11), P. 3569 - 3582

Published: July 26, 2024

Polycystic ovary syndrome (PCOS) is a metabolic disease that affects the reproductive system, and its pathogenesis remains unresolved. Through application of bioinformatics molecular biology techniques, this study has identified significant association between translocase outer mitochondrial membrane 40 (TOMM40) both PCOS pan-cancers. The selection biomarkers included TOMM40, which we found to be significantly decreased in group vitro vivo, using methods such as Western Blot well immunohistochemistry. Over-expression TOMM40 can rescue effect on apoptosis rate proliferation suppression induced by DHEA KGN cells. biomarker for diagnosis PCOS. pan-cancer analysis revealed an elevated expression Uterine Corpus Endometrial Carcinoma unfavorable prognosis, while increased six tumor types was linked favorable prognosis. Therefore, regarded promising diagnosing pan-cancer.

Language: Английский

Citations

0