Advances in genomic tools for plant breeding: harnessing DNA molecular markers, genomic selection, and genome editing DOI Creative Commons
Rahul Kumar,

Sankar Prasad Das,

Burhan U. Choudhury

et al.

Biological Research, Journal Year: 2024, Volume and Issue: 57(1)

Published: Nov. 7, 2024

Abstract Conventional pre-genomics breeding methodologies have significantly improved crop yields since the mid-twentieth century. Genomics provides breeders with advanced tools for whole-genome study, enabling a direct genotype–phenotype analysis. This shift has led to precise and efficient development through genomics-based approaches, including molecular markers, genomic selection, genome editing. Molecular such as SNPs, are crucial identifying regions linked important traits, enhancing accuracy efficiency. Genomic resources viz. genetic reference genomes, sequence protein databases, transcriptomes, gene expression profiles, vital in plant aid identification of key understanding diversity, assist mapping, support marker-assisted selection speeding up programs. Advanced techniques like CRISPR/Cas9 allow modification, accelerating processes. Key Genome-Wide Association study (GWAS), Marker-Assisted Selection (MAS), (GS) enable trait prediction outcomes, improving yield, disease resistance, stress tolerance. These handy complex traits influenced by multiple genes environmental factors. paper explores new technologies editing showcasing their impact on developing varieties.

Language: Английский

ABA Metabolism and Homeostasis in Seed Dormancy and Germination DOI Open Access
Naoto Sano, Annie Marion‐Poll

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(10), P. 5069 - 5069

Published: May 11, 2021

Abscisic acid (ABA) is a key hormone that promotes dormancy during seed development on the mother plant and after dispersal participates in control of release germination response to environmental signals. The modulation ABA endogenous levels largely achieved by fine-tuning, different tissues, synthesis cleavage carotenoid precursors inactivation 8′-hydroxylation. In this review, we provide an overview current knowledge metabolism developing germinating seeds; notably, how signals such as light, temperature nitrate through adjustment levels. A number regulatory factors have been recently identified which functional relationships with major transcription factors, INSENSITIVE3 (ABI3), ABI4 ABI5, essential role increasing importance epigenetic mechanisms regulation gene expression also described. last section, give natural variations genes their effects germination, could be useful both future studies better understand identify candidates breeding materials for improving properties.

Language: Английский

Citations

155
Variation in cis-regulation of a NAC transcription factor contributes to drought tolerance in wheat DOI Creative Commons
Hude Mao, Shumin Li, Bin Chen

et al.

Molecular Plant, Journal Year: 2021, Volume and Issue: 15(2), P. 276 - 292

Published: Nov. 15, 2021

Language: Английский

Citations

151
Complex plant responses to drought and heat stress under climate change DOI Creative Commons
Hikaru Sato, Junya Mizoi, Kazuo Shinozaki

et al.

The Plant Journal, Journal Year: 2024, Volume and Issue: 117(6), P. 1873 - 1892

Published: Jan. 3, 2024

SUMMARY Global climate change is predicted to result in increased yield losses of agricultural crops caused by environmental conditions. In particular, heat and drought stress are major factors that negatively affect plant development reproduction, previous studies have revealed how these stresses induce responses at physiological molecular levels. Here, we provide a comprehensive overview current knowledge concerning drought, heat, combinations conditions the status plants, including crops, affecting such as stomatal conductance, photosynthetic activity, cellular oxidative conditions, metabolomic profiles, signaling mechanisms. We further discuss stress‐responsive regulatory transcription factors, which play critical roles adaptation both potentially function ‘hubs’ and/or responses. Additionally, present recent findings based on forward genetic approaches reveal natural variations traits under Finally, an application decades study results actual fields strategy increase tolerance. This review summarizes our understanding

Language: Английский

Citations

105
Engineering drought and salinity tolerance traits in crops through CRISPR-mediated genome editing: Targets, tools, challenges, and perspectives DOI Creative Commons
Rahul Mahadev Shelake, Ulhas Sopanrao Kadam, Ritesh Kumar

et al.

Plant Communications, Journal Year: 2022, Volume and Issue: 3(6), P. 100417 - 100417

Published: Aug. 3, 2022

Prolonged periods of drought triggered by climate change hamper plant growth and cause substantial agricultural yield losses every year. In addition to drought, salinity is one the major abiotic stresses that severely affect crop health production. Plant responses involve multiple processes operate in a spatiotemporal manner, such as stress sensing, perception, epigenetic modifications, transcription, post-transcriptional processing, translation, post-translational changes. Consequently, tolerance are polygenic traits influenced genome-environment interactions. One ideal solutions these challenges development high-yielding varieties with enhanced tolerance, together improved practices. Recently, genome-editing technologies, especially clustered regularly interspaced short palindromic repeats (CRISPR) tools, have been effectively applied elucidate how plants deal saline environments. this work, we aim portray combined use CRISPR-based genome engineering tools modern genomic-assisted breeding approaches gaining momentum identifying genetic determinants complex for improvement. This review provides synopsis at morphological, physiological, molecular levels. We also highlight recent advances their understanding multi-level nature adaptations stress. Integrating CRISPR factors regulate stress-response pathways introgression beneficial develop stress-resilient crops.

Language: Английский

Citations

95
A gain-of-function allele of a DREB transcription factor gene ameliorates drought tolerance in wheat DOI Open Access
Fangming Mei, Bin Chen, Linying Du

et al.

The Plant Cell, Journal Year: 2022, Volume and Issue: 34(11), P. 4472 - 4494

Published: Aug. 12, 2022

Abstract Drought is a major environmental factor limiting wheat production worldwide. However, the genetic components underlying drought tolerance are largely unknown. Here, we identify DREB transcription gene (TaDTG6-B) by genome-wide association study that tightly associated with in wheat. Candidate analysis revealed 26-bp deletion TaDTG6-B coding region induces gain-of-function for TaDTG6-BDel574, which exhibits stronger transcriptional activation, protein interactions, and binding activity to dehydration-responsive elements (DRE)/CRT cis-elements than TaDTG6-BIn574 encoded allele lacking deletion, thus conferring greater seedlings harboring this variant. Knockdown of TaDTG6-BDel574 transcripts attenuated transgenic wheat, whereas its overexpression resulted enhanced without accompanying phenotypic abnormalities. Furthermore, introgression elite into drought-sensitive cultivars improved their tolerance, providing valuable resource breeding. We also identified 268 putative target genes directly bound transcriptionally regulated TaDTG6-BDel574. Further showed positively regulates TaPIF1 enhance tolerance. These results describe basis mechanism driving variation provide novel crop breeding programs.

Language: Английский

Citations

81
Recent Advances for Drought Stress Tolerance in Maize (Zea mays L.): Present Status and Future Prospects DOI Creative Commons
Seema Sheoran,

Yashmeet Kaur,

Sushil Kumar

et al.

Frontiers in Plant Science, Journal Year: 2022, Volume and Issue: 13

Published: May 30, 2022

Drought stress has severely hampered maize production, affecting the livelihood and economics of millions people worldwide. In future, as a result climate change, unpredictable weather events will become more frequent hence implementation adaptive strategies be inevitable. Through utilizing different genetic breeding approaches, efforts are in progress to develop drought tolerance maize. The recent approaches genomics-assisted breeding, transcriptomics, proteomics, transgenics, genome editing have fast-tracked enhancement for under laboratory field conditions. could considerably improved by combining omics technologies with novel methods high-throughput phenotyping (HTP). This review focuses on responses against drought, well system biology applied better understand mechanisms development drought-tolerant cultivars. Researchers must disentangle molecular physiological bases features order increase yield. Therefore, integrated investments field-based HTP, biology, sophisticated methodologies expected help stabilize production face change.

Language: Английский

Citations

74
Genetic and molecular exploration of maize environmental stress resilience: Toward sustainable agriculture DOI
Zhirui Yang, Yibo Cao, Yiting Shi

et al.

Molecular Plant, Journal Year: 2023, Volume and Issue: 16(10), P. 1496 - 1517

Published: July 18, 2023

Language: Английский

Citations

55
Effects of Climate Change and Drought Tolerance on Maize Growth DOI Creative Commons
Kyung‐Hee Kim,

Byung‐Moo Lee

Plants, Journal Year: 2023, Volume and Issue: 12(20), P. 3548 - 3548

Published: Oct. 12, 2023

Climate change is affecting all regions of the world with different climates, and scale damage increasing due to occurrence various natural disasters. In particular, maize production highly affected by abnormal climate events such as heat waves droughts. Increasing temperatures can accelerate growth shorten growing season, potentially reducing productivity. Additionally, enhanced during ripening period process, crop yields. addition, drought stress water deficit greatly affect seedling formation, early plant growth, photosynthesis, reproductive yield, so proper management critical growth. Maize, in tall broad-leaved, extreme at planting cause leaves curl stunt It important understand that severe have a detrimental effect on reproduction maize. high caused inhibit induction flowering male flowers factors interfere pollen development. therefore increase productivity food crops, including maize, while maintaining them face persistent change. This requires strategy develop genetically modified crops drought-tolerant effectively respond The aim this paper investigate effects tolerance We also reviewed molecular breeding techniques varieties response

Language: Английский

Citations

52
Genome assembly and genetic dissection of a prominent drought-resistant maize germplasm DOI
Tian Tian, Shuhui Wang, Shiping Yang

et al.

Nature Genetics, Journal Year: 2023, Volume and Issue: 55(3), P. 496 - 506

Published: Feb. 20, 2023

Language: Английский

Citations

51
Advancements and Prospects of Genome-Wide Association Studies (GWAS) in Maize DOI Open Access

Javed Hussain Sahito,

Hao Zhang,

Zeeshan Ghulam Nabi Gishkori

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(3), P. 1918 - 1918

Published: Feb. 5, 2024

Genome-wide association studies (GWAS) have emerged as a powerful tool for unraveling intricate genotype-phenotype across various species. Maize (

Language: Английский

Citations

27