Integration of high-throughput phenotyping, GWAS, and predictive models reveals the genetic architecture of plant height in maize

Molecular Plant, Journal Year: 2022, Volume and Issue: 16(2), P. 354 - 373

Published: Nov. 29, 2022

Plant height (PH) is an essential trait in maize (Zea mays) that tightly associated with planting density, biomass, lodging resistance, and grain yield the field. Dissecting dynamics of plant architecture will be beneficial for ideotype-based breeding prediction, as genetic basis controlling PH remains largely unknown. In this study, we developed automated high-throughput phenotyping platform (HTP) to systematically noninvasively quantify 77 image-based traits (i-traits) 20 field (f-traits) 228 inbred lines across all developmental stages. Time-resolved i-traits novel digital phenotypes complex correlations agronomic were characterized reveal growth. An i-trait-based genome-wide association study identified 4945 trait-associated SNPs, 2603 loci, 1974 corresponding candidate genes. We found rapid growth plants occurs mainly at two stages, stage 2 (S2) S3 S5 S6, accounting final indicators. By integrating PH-association network transcriptome profiles specific internodes, revealed 13 hub genes may play vital roles during The multiple stages used potential indicators maize. One gene, ZmVATE, was functionally validated shown regulate PH-related using mutation. Furthermore, machine learning build predictive models based on i-traits, their performance assessed Moderate, strong, very strong between predictions experimental datasets achieved from early S4 (tenth-leaf) stage. Colletively, our provides a valuable tool dissecting spatiotemporal formation internodes PH, well resources are useful molecular design predicting varieties ideal architectures.

Language: Английский

---

Engineering Abiotic Stress Tolerance in Crop Plants through CRISPR Genome Editing

Cells, Journal Year: 2022, Volume and Issue: 11(22), P. 3590 - 3590

Published: Nov. 13, 2022

Environmental abiotic stresses challenge food security by depressing crop yields often exceeding 50% of their annual production. Different methods, including conventional as well genomic-assisted breeding, mutagenesis, and genetic engineering have been utilized to enhance stress resilience in several species. Plant breeding has partly successful developing varieties against owning the complex genetics traits narrow base germplasm. Irrespective fact that can transfer gene(s) from any organism(s), transgenic crops become controversial mainly due potential risk transgene-outcrossing. Consequently, cultivation is banned certain countries, particularly European countries. In this scenario, discovery CRISPR tool provides a platform for producing transgene-free genetically edited plants—similar mutagenized are not extensively regulated such modified organisms (GMOs). Thus, genome-edited plants without transgene would likely go into field restriction. Here, we focused on deployment development stress-tolerant sustaining productivity under changing environments.

Language: Английский

---

Allelic variation of TaWD40-4B.1 contributes to drought tolerance by modulating catalase activity in wheat

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: March 2, 2023

Abstract Drought drastically restricts wheat production, so to dissect allelic variations of drought tolerant genes without imposing trade-offs between tolerance and yield is essential cope with the circumstance. Here, we identify a WD40 protein encoding gene TaWD40-4B.1 via genome-wide association study. The full-length allele C but not truncated T possessing nonsense nucleotide variation enhances grain under drought. interacts canonical catalases, promotes their oligomerization activities, reduces H 2 O levels knock-down catalase erases role in tolerance. proportion accessions negatively correlative annual rainfall, suggesting this may be selected during breeding. introgression cultivar harboring . Therefore, could useful for molecular breeding wheat.

Language: Английский

---

ABA‐inducible DEEPER ROOTING1 improves adaptation of maize to water deficiency

Plant Biotechnology Journal, Journal Year: 2022, Volume and Issue: 20(11), P. 2077 - 2088

Published: July 7, 2022

Root architecture remodelling is critical for forage moisture in water-limited soil. DEEPER ROOTING 1 (DRO1) Oryza, Arabidopsis, and Prunus has been reported to improve drought avoidance by promoting roots grow downward acquire water from deeper In the present study, we found that ZmDRO1 responded more strongly abscisic acid (ABA)/drought induction Zea mays ssp. mexicana, an ancestral species of cultivated maize, than B73. It was proposed this one reasons why mexicana a noticeable change direction angle root fewer biomass penalties under water-deficient conditions. Thus, robust, synthetic ABA/drought-inducible promoter used control expression ZmDRO1B73 Arabidopsis maize drought-resistant breeding. Interestingly, ABA-inducible promoted larger improved grain yield 40% Collectively, these results revealed different responses ABA/drought confer abilities, demonstrated application via strategy alter modern adaptation field.

Language: Английский

---

Gene Editing for Plant Resistance to Abiotic Factors: A Systematic Review

Plants, Journal Year: 2023, Volume and Issue: 12(2), P. 305 - 305

Published: Jan. 9, 2023

Agricultural crops are exposed to various abiotic stresses, such as salinity, water deficits, temperature extremes, floods, radiation, and metal toxicity. To overcome these challenges, breeding programs seek improve methods techniques. Gene editing by Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR/Cas-is a versatile tool for in all layers of the central dogma with focus on development cultivars plants resistant or tolerant multiple biotic stresses. This systematic review (SR) brings new contributions study use CRISPR/Cas gene tolerance stress plants. Articles deposited different electronic databases, using search

Language: Английский

---