Major viral diseases in grain legumes: designing disease resistant legumes from plant breeding and OMICS integration DOI Creative Commons
Uday Chand Jha, Harsh Nayyar, Anirudha Chattopadhyay

et al.

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: May 9, 2023

Grain legumes play a crucial role in human nutrition and as staple crop for low-income farmers developing underdeveloped nations, contributing to overall food security agroecosystem services. Viral diseases are major biotic stresses that severely challenge global grain legume production. In this review, we discuss how exploring naturally resistant genotypes within germplasm, landraces, wild relatives could be used promising, economically viable, eco-environmentally friendly solution reduce yield losses. Studies based on Mendelian classical genetics have enhanced our understanding of key genetic determinants govern resistance various viral legumes. Recent advances molecular marker technology genomic resources enabled us identify regions controlling disease using techniques such QTL mapping, genome-wide association studies, whole-genome resequencing, pangenome ‘omics’ approaches. These comprehensive expedited the adoption genomics-assisted breeding virus-resistant Concurrently, progress functional genomics, especially transcriptomics, has helped unravel underlying candidate gene(s) their roles This review also examines engineering-based strategies, including RNA interference, potential synthetic biology techniques, promoters transcription factors, creating viral-resistant It elaborates prospects limitations cutting-edge technologies emerging biotechnological tools (e.g., selection, rapid generation advances, CRISPR/Cas9-based genome editing tool) virus-disease-resistant ensure security.

Language: Английский

The CRISPR-Cas toolbox and gene editing technologies DOI Creative Commons

Guanwen Liu,

Qiupeng Lin, Shuai Jin

et al.

Molecular Cell, Journal Year: 2021, Volume and Issue: 82(2), P. 333 - 347

Published: Dec. 29, 2021

Language: Английский

Citations

260
Host-mediated gene engineering and microbiome-based technology optimization for sustainable agriculture and environment DOI Open Access
Nitika Thakur,

Mohit Nigam,

Neharika Ann Mann

et al.

Functional & Integrative Genomics, Journal Year: 2023, Volume and Issue: 23(1)

Published: Feb. 8, 2023

Language: Английский

Citations

55
Agricultural biotechnology for sustainable food security DOI Creative Commons
Agata Tyczewska, Tomasz Twardowski, Ewa Woźniak

et al.

Trends in biotechnology, Journal Year: 2023, Volume and Issue: 41(3), P. 331 - 341

Published: Jan. 28, 2023

Of late, global food security has been under threat by the coronavirus disease 2019 (COVID-19) pandemic and recent military conflict in Eastern Europe. This article presents objectives of Sustainable Development Goals European Green Deal related to achieving sustainable development Union (EU) agriculture, taking aforementioned threats into account. In addition, it discusses future plant agricultural biotechnology artificial intelligence (AI) systems, considering their potential for reaching goal security. Paradoxically, present challenging situation may allow politicians stakeholders EU realize opportunities use sector.

Language: Английский

Citations

46
Harnessing genetic engineering to drive economic bioproduct production in algae DOI Creative Commons
Abhishek Gupta, Kalisa Kang, Ruchi Pathania

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12

Published: Jan. 29, 2024

Our reliance on agriculture for sustenance, healthcare, and resources has been essential since the dawn of civilization. However, traditional agricultural practices are no longer adequate to meet demands a burgeoning population amidst climate-driven challenges. Microalgae emerge as beacon hope, offering sustainable renewable source food, animal feed, energy. Their rapid growth rates, adaptability non-arable land non-potable water, diverse bioproduct range, encompassing biofuels nutraceuticals, position them cornerstone future resource management. Furthermore, microalgae’s ability capture carbon aligns with environmental conservation goals. While microalgae offers significant benefits, obstacles in cost-effective biomass production persist, which curtails broader application. This review examines compared other host platforms, highlighting current innovative approaches aimed at overcoming existing barriers. These include range techniques, from gene editing, synthetic promoters, mutagenesis selective breeding metabolic engineering through transcription factors.

Language: Английский

Citations

20
CRISPR-Cas9 genome editing in crop breeding for climate change resilience: Implications for smallholder farmers in Africa DOI Creative Commons
Abigarl Ndudzo,

Angela Sibanda Makuvise,

S. Moyo

et al.

Journal of Agriculture and Food Research, Journal Year: 2024, Volume and Issue: 16, P. 101132 - 101132

Published: March 28, 2024

Food insecurity and malnutrition, compounded by climate change, are seriously threatening the growing African population. Unpredictable precipitation patterns droughts contributing to declining crop productivity. Efforts increase agricultural productivity include adoption of crops that resistant change engaging in resilient agriculture. Currently, CRISPR-Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats - associated protein) technology is being used breeding practices improve traits such as drought tolerance, nutrition disease resistance. The aim this review explore use developing for mitigation food hunger attendant implications small holder farmers Africa. A comprehensive systematic search peer reviewed articles three bibliographic databases, PubMed, Web Science Google Scholar, was conducted identify documents relevant applications CRISPR-mediated genome editing improvement context. literature shows CRISPR-Cas has been harnessed enhance resilience nutritional content various crops, combatting biotic abiotic stresses. CRISPR-assisted strategies can help smallholder low-middle income countries Africa adapt without loss. By harnessing technology, benefit from with improved yields stress resistance thereby security sustainable agriculture on continent.

Language: Английский

Citations

20
SYNCAS: Efficient CRISPR/Cas9 gene-editing in difficult to transform arthropods DOI Creative Commons
Sander De Rouck, Antonio Mocchetti, Wannes Dermauw

et al.

Insect Biochemistry and Molecular Biology, Journal Year: 2024, Volume and Issue: 165, P. 104068 - 104068

Published: Jan. 1, 2024

The genome editing technique CRISPR/Cas9 has led to major advancements in many research fields and this state-of-the-art tool proven its use genetic studies for various arthropods. However, most transformation protocols rely on microinjection of component into embryos, a method which is challenging species. Alternatively, injections can be performed adult females, but efficiencies very low as was shown the two-spotted spider mite, Tetranychus urticae, minute important chelicerate pest crops. In study, we explored different formulations optimize maternal injection protocol T. urticae. We observed strong synergy between branched amphipathic peptide capsules saponins, resulting significant increase knock-out efficiency, exceeding 20%. This formulation, termed SYNCAS, used urticae genes – phytoene desaturase, CYP384A1 Antennapedia also allowed develop co-CRISPR strategy facilitated generation knock-in mutants. addition, SYNCAS successfully applied white white-like western flower thrips, Frankliniella occidentalis. allows routine these species game changer other hard transform

Language: Английский

Citations

19
Fostering plant growth performance under drought stress using rhizospheric microbes, their gene editing, and biochar DOI

Prabhat K. Chauhan,

Sudhir K. Upadhyay, Vishnu D. Rajput

et al.

Environmental Geochemistry and Health, Journal Year: 2024, Volume and Issue: 46(2)

Published: Jan. 16, 2024

Language: Английский

Citations

18
CRISPR–Cas-mediated chromosome engineering for crop improvement and synthetic biology DOI
Michelle Rönspies, A Dorn, Patrick Schindele

et al.

Nature Plants, Journal Year: 2021, Volume and Issue: 7(5), P. 566 - 573

Published: May 6, 2021

Language: Английский

Citations

71
Advances in Crop Breeding Through Precision Genome Editing DOI Creative Commons
Gauri Nerkar,

Suman Devarumath,

Madhavi V. Purankar

et al.

Frontiers in Genetics, Journal Year: 2022, Volume and Issue: 13

Published: July 14, 2022

The global climate change and unfavourable abiotic biotic factors are limiting agricultural productivity therefore intensifying the challenges for crop scientists to meet rising demand food supply. introduction of applied genetics agriculture through plant breeding facilitated development hybrid varieties with improved productivity. However, new existing gene pools poses a challenge breeders. Genetic engineering holds potential broaden genetic diversity by genes into crops. But random insertion foreign DNA plant’s nuclear genome often leads transgene silencing. Recent advances in field include technique called editing. Genome editing technologies have emerged as powerful tools precisely modify genomes at specific sites genome, which has been longstanding goal precise modification target absence genome-edited plants, faster cheaper method remarkable features genome-editing technology that resulted its widespread application less than decade. This review focuses on precision includes: an overview different approaches improvement; their mechanism action most widely used technology, CRISPR/Cas9, improvement especially agronomic traits such disease resistance, stress tolerance, herbicide yield quality improvement, reduction anti-nutrients, shelf life; update regulatory approval also throws light high-yielding climate-resilient crops

Language: Английский

Citations

58
Gene Editing for Plant Resistance to Abiotic Factors: A Systematic Review DOI Creative Commons
Fernanda dos Santos Nascimento, Anelita de Jesus Rocha, Julianna Matos da Silva Soares

et al.

Plants, Journal Year: 2023, Volume and Issue: 12(2), P. 305 - 305

Published: Jan. 9, 2023

Agricultural crops are exposed to various abiotic stresses, such as salinity, water deficits, temperature extremes, floods, radiation, and metal toxicity. To overcome these challenges, breeding programs seek improve methods techniques. Gene editing by Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR/Cas-is a versatile tool for in all layers of the central dogma with focus on development cultivars plants resistant or tolerant multiple biotic stresses. This systematic review (SR) brings new contributions study use CRISPR/Cas gene tolerance stress plants. Articles deposited different electronic databases, using search

Language: Английский

Citations

36