Genome-Wide Identification and Analysis of the MYC Gene Family in Cotton: Evolution and Expression Profiles During Normal Growth and Stress Response DOI Open Access
Jingxi Chen, Long Wang, Xiufang Wang

et al.

Genes, Journal Year: 2024, Volume and Issue: 16(1), P. 20 - 20

Published: Dec. 26, 2024

Background: The gene family of myelomatosis (MYC), serving as a transcription factor in the jasmonate (JA) signaling pathway, displays significant level conservation across diverse animal and plant species. Cotton is most widely used for fiber production. Nevertheless, there paucity literature reporting on members MYCs how they respond to biotic stresses cotton. Methods: Bioinformatics analysis was mine MYC cotton based InterPro, cottongen, etc. Results: structure, conserved motifs, upstream open reading frames 32 GhMYCs Gossypium hirsutum were identified. Moreover, it anticipated that GT1-motif abundant GhMYCs, indicating plays role light-responsive GhMYCs. expression patterns under including V. dahliae Aphid gossypii evaluated, suggesting class-1 -3 which function negative regulators, are involved resistance verticillium wilt aphids. class-3 genes found be mostly expressed female tissues. Interestingly, also determined homeologous bias within uncovered, results showed class-1A class-2 Dt sub-genome may have direct impact function. Conclusions: This study provides research direction researchers breeders enhance traits through manipulating individual or multiple homeologs, laid foundation further molecular characteristics biological functions GhMYC gene.

Language: Английский

Precise fine-turning of GhTFL1 by base editing tools defines ideal cotton plant architecture DOI Creative Commons
Guanying Wang, Fuqiu Wang, Zhongping Xu

et al.

Genome biology, Journal Year: 2024, Volume and Issue: 25(1)

Published: Feb. 26, 2024

CRISPR/Cas-derived base editor enables precise editing of target sites and has been widely used for basic research crop genetic improvement. However, the efficiency editors at different targets varies greatly.

Language: Английский

Citations

20
CRISPR/Cas genome editing for cotton precision breeding: mechanisms, advances, and prospects DOI Creative Commons
Vijay Sheri, M. Harikrishnan, Phanikanth Jogam

et al.

Journal of Cotton Research, Journal Year: 2025, Volume and Issue: 8(1)

Published: Feb. 3, 2025

Abstract Cotton ( Gossypium hirsutum L.) is one of the most important global crops that supports textile industry and provides a living for millions farmers. The constantly increasing demand needs significant rise in cotton production. Genome editing technology, specifically with clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) tools, has opened new possibilities trait development cotton. It allows precise efficient manipulation within genome when compared other genetic engineering tools. Current developments CRISPR/Cas including prime editing, base multiplexing have expanded scope traits breeding can be targeted. been employed to generate effectively CRISPRized plants enhanced agronomic traits, fiber yield quality, oil improvement, stress resistance, nutrition. Here we summarized various target genes which successfully altered However, some challenges remain, tetraploid having redundant gene sets homologs making editing. To ensure specificity avoiding off-target effects, need optimize parameters such as site, guide RNA design, choosing right Cas variants. We outline future prospects breeding, suggesting areas further research innovation. A combination speed might useful fastening potentials create customized cultivars meet higher demands agriculture industry.

Language: Английский

Citations

1
Unlocking Nature’s Shield: The Promising Potential of CRISPRa in Amplifying Antimicrobial Peptide Expression in Common Bean (Phaseolus vulgaris L.) DOI Creative Commons
Mariana Rocha Maximiano, Lucas José de Sousa,

Gabriel Cidade Feitosa

et al.

ACS Omega, Journal Year: 2025, Volume and Issue: 10(6), P. 5909 - 5918

Published: Feb. 6, 2025

This study proposes using the CRISPR transcriptional activation strategy to modulate expression of genes encoding defense proteins and antimicrobial peptides (AMPs) in Phaseolus vulgaris. Three (PvD1, Pv-thionin, Pv-lectin) were selected targeted by CRISPR-dCas9-TV-mediated complex P. vulgaris L. hairy root. RT-qPCR investigated their efficiency. The eGFP-positive transgenic roots exhibit enhanced compared that control roots. A moderate increase 1.37-fold PvD1 gene was observed roots, while 6.97-fold (Pv-lectin) 5.70-fold (Pv-thionin) increases observed. Importantly, no off-target effects sgRNAs detected, ensuring precision safety CRISPR-dCas9-TV strategy. present article is a proof-of-concept study, it has succeeded demonstrating efficiency modulating target vulgaris, paving way for an alternative approach protecting such essential crop plants.

Language: Английский

Citations

0
Genome-wide identification unravels the role of the arabinogalactan peptide (AGP) gene family in cotton plant architecture DOI
Jun Tang, Teame Gereziher Mehari, David C. Qian

et al.

Plant Cell Reports, Journal Year: 2025, Volume and Issue: 44(4)

Published: March 8, 2025

Language: Английский

Citations

0
CRISPR/Cas9: a sustainable technology to enhance climate resilience in major Staple Crops DOI Creative Commons
Navjot Kaur, Muslim Qadir, Dali Vilma Francis

et al.

Frontiers in Genome Editing, Journal Year: 2025, Volume and Issue: 7

Published: March 18, 2025

Climate change is a global concern for agriculture, food security, and human health. It affects several crops causes drastic losses in yield, leading to severe disturbances the economy, environment, community. The consequences on important staple crops, such as rice, maize, wheat, will worsen create insecurity across globe. Although various methods of trait improvements are available being used, clustered regularly interspaced short palindromic repeats CRISPR-associated protein 9 (CRISPR/Cas9) mediated genome manipulation have opened new avenue functional genomics crop improvement. This review discuss progression improvement from conventional breeding advanced editing techniques how CRISPR/Cas9 technology can be applied enhance tolerance main cereal (wheat, maize) against any harsh climates. CRISPR/Cas endonucleases their derived genetic engineering tools possess high accuracy, versatile, more specific, easy design, climate-smart or resilient combat survive environments. CRISPR/Cas9-mediated approach has been make them climate resilient. review, supported by bibliometric analysis recent literature, highlights potential target genes/traits addresses significance gene technologies tackling vulnerable effects major maize.

Language: Английский

Citations

0
CRISPR plants for studying functional genomics: Methods and applications DOI
Mireia Uranga, Ana Montserrat Martín‐Hernández, Fabio Pasin

et al.

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 27 - 59

Published: Jan. 1, 2025

Language: Английский

Citations

0
CRISPR/Cas9 mediated genome editing for crop improvement against Abiotic stresses: current trends and prospects DOI

Mestawut Adane,

Getachew Alamnie

Functional & Integrative Genomics, Journal Year: 2024, Volume and Issue: 24(6)

Published: Oct. 25, 2024

Language: Английский

Citations

2
Unlocking crops’ genetic potential: Advances in genome and epigenome editing of regulatory regions DOI

Namra Ali,

Shubhangi Singh,

Rohini Garg

et al.

Current Opinion in Plant Biology, Journal Year: 2024, Volume and Issue: 83, P. 102669 - 102669

Published: Nov. 26, 2024

Language: Английский

Citations

2
Advancements of CRISPR-Mediated Base Editing in Crops and Potential Applications in Populus DOI Open Access
Xuefei Yang, Ping Zhu, Jinshan Gui

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(15), P. 8314 - 8314

Published: July 30, 2024

Base editing represents a cutting-edge genome technique that utilizes the CRISPR system to guide base deaminases with high precision specific genomic sites, facilitating targeted alteration of individual nucleotides. Unlike traditional gene approaches, does not require DNA double-strand breaks or donor templates. It functions independently cellular repair machinery, offering significant advantages in terms both efficiency and accuracy. In this review, we summarize core design principles various editors, their distinctive characteristics, tactics refine efficacy. We also applications crop genetic improvement explore potential contributions forest engineering.

Language: Английский

Citations

1
The 4Fs of cotton: genome editing of cotton for fiber, food, feed, and fuel to achieve zero hunger DOI Creative Commons

Muhammad Sulyman Saleem,

Sultan Habibullah Khan, Aftab Ahmad

et al.

Frontiers in Genome Editing, Journal Year: 2024, Volume and Issue: 6

Published: Sept. 12, 2024

Cotton is globally known for its high-priority cellulose-rich natural fiber. In addition to providing fiber the textile industry, it an important source material edible oil, livestock feed, and fuel products. Global warming growing population are major challenges world's agriculture potential risks food security. this context, improving output traits in cotton necessary achieve sustainable production. During last few years, high throughput omics techniques have aided identifying crucial genes associated with of fiber, seed, plant architecture which could be targeted more precision efficiency through CIRPSR/Cas-mediated genome editing technique. The various CRISPR/Cas systems such as CRISPR/Cas9, CRISPR/nCas9, CRISPR/Cas12a been employed edit a wide range including length, flowering, leaf colour, rooting, seed architecture, gossypol content, somatic embryogenesis, biotic abiotic stresses tolerance, highlighting effectiveness genome. Thus, CRISPR/Cas-mediated has emerged technique choice tailor crop phenotypes better yield environmental resilience. review covers comprehensive analysis phenotypic their improvement help latest tools improve food, fuel-associated ensure

Language: Английский

Citations

1