Transitioning from the Green Revolution to the Gene Revolution: strengthening nutritional security using climate resilient traditional crops DOI Creative Commons
Rushikesh Tahakik,

Amit G Deshmukh,

M. P. Moharil

et al.

Bulletin of the National Research Centre/Bulletin of the National Research Center, Journal Year: 2024, Volume and Issue: 48(1)

Published: Dec. 9, 2024

Abstract Background World food production has significantly increased, largely attributed to advancements in breeding methods pioneered previous decades. These can be categorized into three major eras—the pre-Green Revolution era, the Green and post-Green era—which we call Gene Revolution. The era was characterized by utilization of mutation forward genetics approaches. Main body (1950s–1980s) brought about improvements crop breeding, irrigation, fertilizer use, leading a substantial rise reduced rural poverty. However, focus primarily on yield, with less nutritional quality. After Revolution, wheat rice rapidly increased from 0.4–2 metric tonnes per hectare 2–4 due government initiatives. Nevertheless, cultivation other crops, including indigenous varieties millet, decreased. In while advanced genome editing technologies such as CRISPR‒Cas9 TALENs have enhanced content climate resilience there also been renewed reviving crops. Conclusion This review discusses bring these orphan nutria crops back emphasizes importance passing this knowledge future generations, ensuring their place sustainable agriculture security.

Language: Английский

Recent advances of CRISPR-based genome editing for enhancing staple crops DOI Creative Commons
Feng Chen, Chen D. Lu, Yan Zhao

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 23, 2024

An increasing population, climate change, and diminishing natural resources present severe threats to global food security, with traditional breeding genetic engineering methods often falling short in addressing these rapidly evolving challenges. CRISPR/Cas systems have emerged as revolutionary tools for precise modifications crops, offering significant advancements resilience, yield, nutritional value, particularly staple crops like rice maize. This review highlights the transformative potential of technology, emphasizing recent innovations such prime base editing, development novel CRISPR-associated proteins, which significantly improved specificity, efficiency, scope genome editing agriculture. These enable targeted that enhance tolerance abiotic stresses well biotic stresses. Additionally, plays a crucial role improving crop yield quality by enhancing photosynthetic nutrient uptake, resistance lodging, while also taste, texture, shelf life, content through biofortification. Despite challenges off-target effects, need more efficient delivery methods, ethical regulatory concerns, underscores importance security sustainability It calls continued research integration CRISPR other emerging technologies nanotechnology, synthetic biology, machine learning fully realize its developing resilient, productive, sustainable agricultural systems.

Language: Английский

Citations

20
CRISPR–Cas applications in agriculture and plant research DOI
Aytug Tuncel, Changtian Pan,

Joshua S. Clem

et al.

Nature Reviews Molecular Cell Biology, Journal Year: 2025, Volume and Issue: unknown

Published: March 7, 2025

Language: Английский

Citations

5
Conditional knockdown of OsMLH1 to improve plant prime editing systems without disturbing fertility in rice DOI Creative Commons
Xiaoshuang Liu,

Dongfang Gu,

Yiru Zhang

et al.

Genome biology, Journal Year: 2024, Volume and Issue: 25(1)

Published: May 21, 2024

Abstract Background High-efficiency prime editing (PE) is desirable for precise genome manipulation. The activity of mammalian PE systems can be largely improved by inhibiting DNA mismatch repair coexpressing a dominant-negative variant MLH1. However, this strategy has not been widely used optimization in plants, possibly because its less conspicuous effects and inconsistent performance at different sites. Results We show that direct RNAi knockdown OsMLH1 an ePE5c system increases the efficiency our most recently updated tool 1.30- to 2.11-fold stably transformed rice cells, resulting as many 85.42% homozygous mutants T 0 generation. high specificity revealed whole-genome sequencing. To overcome partial sterility induced ePE5c, conditional excision introduced remove module Cre-mediated site-specific recombination. Using simple approach enriching events, we generate 100% module-free plants increase due maintained excised whose fertility impaired. Conclusions This study provides safe reliable plant improving without disturbing development via transient MMR inhibition with excisable

Language: Английский

Citations

13
Emerging applications of gene editing technologies for the development of climate-resilient crops DOI Creative Commons
R. L. Chavhan,

Siddhant Gahininath Jaybhaye,

V. R. Hinge

et al.

Frontiers in Genome Editing, Journal Year: 2025, Volume and Issue: 7

Published: March 10, 2025

Climate change threatens global crop yield and food security due to rising temperatures, erratic rainfall, increased abiotic stresses like drought, heat, salinity. Gene editing technologies, including CRISPR/Cas9, base editors, prime offer precise tools for enhancing resilience. This review explores the mechanisms of these technologies their applications in developing climate-resilient crops address future challenges. While CRISPR/enables targeted modifications plant DNA, editors allow direct conversion without inducing double-stranded breaks, enable insertions, deletions, substitutions. By understanding manipulating key regulator genes involved stress responses, such as DREB, HSP, SOS, ERECTA, HsfA1, NHX; tolerance can be enhanced against salt stress. improve traits related root development, water use efficiency, response pathways, heat shock response, photosynthesis, membrane stability, ion homeostasis, osmotic adjustment, oxidative response. Advancements gene integration with genomics, phenomics, artificial intelligence (AI)/machine learning (ML) hold great promise. However, challenges off-target effects, delivery methods, regulatory barriers must addressed. highlights potential develop crops, contributing sustainable agriculture.

Language: Английский

Citations

1
PE6c greatly enhances prime editing in transgenic rice plants DOI Creative Commons
Zhenghong Cao, Wei Sun, Dexin Qiao

et al.

Journal of Integrative Plant Biology, Journal Year: 2024, Volume and Issue: 66(9), P. 1864 - 1870

Published: July 9, 2024

ABSTRACT Prime editing is a versatile CRISPR/Cas‐based precise genome‐editing technique for crop breeding. Four new types of prime editors (PEs) named PE6a–d were recently generated using evolved and engineered reverse transcriptase (RT) variants from three different sources. In this study, we tested the efficiencies four PE6 two additional constructs with double‐RT modules in transgenic rice ( Oryza sativa ) plants. PE6c, an RT variant yeast Tf1 retrotransposon, yielded highest prime‐editing efficiency. The average fold change efficiency PE6c compared PEmax exceeded 3.5 across 18 agronomically important target sites 15 genes. We also demonstrated feasibility to improve Our results suggest that or its derivatives would be excellent choice monocot addition, our findings have laid foundation prime‐editing‐based breeding varieties enhanced traits.

Language: Английский

Citations

6
CRISPR-mediated genome editing of wheat for enhancing disease resistance DOI Creative Commons
Joshua Waites, V. Mohan Murali Achary, Easter D. Syombua

et al.

Frontiers in Genome Editing, Journal Year: 2025, Volume and Issue: 7

Published: Feb. 25, 2025

Wheat is cultivated across diverse global environments, and its productivity significantly impacted by various biotic stresses, most importantly but not limited to rust diseases, Fusarium head blight, wheat blast, powdery mildew. The genetic diversity of modern cultivars has been eroded domestication selection, increasing their vulnerability stress due uniformity. rapid spread new highly virulent aggressive pathogen strains exacerbated this situation. Three strategies can be used for enhancing disease resistance through genome editing: introducing (R) gene-mediated resistance, engineering nucleotide-binding leucine-rich repeat receptors (NLRs), manipulating susceptibility (S) genes stop pathogens from exploiting these factors support infection. Utilizing R the common strategy traditional breeding approaches, continuous evolution effectors eventually overcome resistance. Moreover, modifying S confer pleiotropic effects that hinder use in agriculture. Enhancing paramount sustainable production food security, tools are great importance research community. application CRISPR-based editing provides promise improve allowing access a broader range solutions beyond random mutagenesis or intraspecific variation, unlocking ways crops, speeding up breeding. Here, we first summarize major context important diseases limitations. Next, turn our attention powerful applications technology creating varieties against diseases.

Language: Английский

Citations

0
Genome Editing: An Approach to Engineer Crops to Withstand Climate Change and Sustain Food Security DOI
Othman Al‐Dossary

Published: Jan. 1, 2025

Language: Английский

Citations

0
Improving plant C-to-G base editors with a cold-adapted glycosylase and TadA-8e variants DOI
Yingli Jiang, Xiao Zhi,

Zhaopeng Luo

et al.

Trends in biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
Establishment of a novel prime editing system by introducing the reverse transcriptase Tf1 combined with high-temperature treatment in rice DOI

Bingbing Hou,

Like Chen,

Hongwei Lu

et al.

Chinese Science Bulletin (Chinese Version), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Language: Английский

Citations

1
Prime editing: A gene precision editing tool from inception to present DOI Open Access

Zhihao Liu,

Dong Guo, Dawei Wang

et al.

The FASEB Journal, Journal Year: 2024, Volume and Issue: 38(21)

Published: Nov. 12, 2024

Genetic mutations significantly contribute to the onset of diseases, with over half cases caused by single-nucleotide mutations. Advances in gene editing technologies have enabled precise and correction mutated genes, offering effective treatment methods for genetic disorders. CRISPR/Cas9, despite its power, poses risks inducing due DNA double-strand breaks (DSB). The advent base (BE) prime (PE) has mitigated these eliminating hazards associated DSBs, allowing more editing. This breakthrough lays a solid foundation clinical application technologies. review discusses principles, development, applications PE technology various mutation-induced diseases.

Language: Английский

Citations

1