Resolving the design principles that control postnatal vascular growth and scaling DOI Open Access
Danielle Pi,

Jonas Braun,

Sayantan Dutta

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 11, 2024

SUMMARY After birth, tissues grow continuously until reaching adult size, with each organ exhibiting unique cellular dynamics, growth patterns, and (stem or non-stem) cell sources. Using a suite of experimental computational multiscale approaches, we found that aortic expansion is guided by specific biological principles scales the vertebral column rather than animal body weight. Expansion proceeds via two distinct waves arterial proliferation along blood flow are spatially stochastic, yet temporally coordinated. Each wave exhibits cycle kinetics properties, first durations as fast 6 hours. Single-cell RNA sequencing showed changes in fatty acid metabolism concomitant an increase size. Mathematical modeling experiments indicated endothelial extrusion essential for homeostatic balancing excess proliferation. In genetic model achondroplasia, aorta achieves proper scaling through enhanced while maintaining normal dynamics. Collectively, these results provide blueprint orchestrate which depends entirely on differentiated resident stem cells.

Language: Английский

Synthetic DNA barcodes identify singlets in scRNA-seq datasets and evaluate doublet algorithms DOI Creative Commons
Ziyang Zhang, Madeline E. Melzer,

Keerthana M. Arun

et al.

Cell Genomics, Journal Year: 2024, Volume and Issue: 4(7), P. 100592 - 100592

Published: June 25, 2024

Language: Английский

Citations

6
Resolving the design principles that control postnatal vascular growth and scaling DOI Open Access
Danielle Pi,

Jonas Braun,

Sayantan Dutta

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 11, 2024

SUMMARY After birth, tissues grow continuously until reaching adult size, with each organ exhibiting unique cellular dynamics, growth patterns, and (stem or non-stem) cell sources. Using a suite of experimental computational multiscale approaches, we found that aortic expansion is guided by specific biological principles scales the vertebral column rather than animal body weight. Expansion proceeds via two distinct waves arterial proliferation along blood flow are spatially stochastic, yet temporally coordinated. Each wave exhibits cycle kinetics properties, first durations as fast 6 hours. Single-cell RNA sequencing showed changes in fatty acid metabolism concomitant an increase size. Mathematical modeling experiments indicated endothelial extrusion essential for homeostatic balancing excess proliferation. In genetic model achondroplasia, aorta achieves proper scaling through enhanced while maintaining normal dynamics. Collectively, these results provide blueprint orchestrate which depends entirely on differentiated resident stem cells.

Language: Английский

Citations

0