Single-cell genomic profiling to study regeneration

Current Opinion in Genetics & Development, Journal Year: 2024, Volume and Issue: 87, P. 102231 - 102231

Published: July 24, 2024

Regenerative capacities and strategies vary dramatically across animals, as well between cell types, organs, with age. In recent years, high-throughput single-cell transcriptomics other profiling technologies have been applied to many animal models gain an understanding of the cellular molecular mechanisms underlying regeneration. Here, we review studies regeneration in diverse contexts summarize key concepts that emerged. The immense regenerative capacity some invertebrates, exemplified by planarians, is driven mainly differentiation abundant adult pluripotent stem cells, whereas cases, involves reactivation embryonic or developmental gene-regulatory networks differentiated types. However, also differs from development ways, including use regeneration-specific types gene regulatory networks.

Language: Английский

Tail Tales: What We Have Learned About Regeneration from Xenopus Laevis Tadpoles

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(21), P. 11597 - 11597

Published: Oct. 29, 2024

This review explores the regenerative capacity of Xenopus laevis, focusing on tail regeneration, as a model to uncover cellular, molecular, and developmental mechanisms underlying tissue repair. X. laevis tadpoles provide unique insights into biology due their regeneration-competent -incompetent stages ability regrow complex structures in tail, including spinal cord, muscle, skin, after amputation. The delves roles key signaling pathways, such those involving reactive oxygen species (ROS) molecules like BMPs FGFs, orchestrating cellular responses during regeneration. It also examines how mechanotransduction, epigenetic regulation, metabolic shifts influence restoration. Comparisons with other shed light evolutionary loss abilities underscore an invaluable for understanding constraints repair higher organisms. comprehensive synthesizes recent findings, suggesting future directions exploring regeneration mechanisms, potential implications advancing medicine.

Language: Английский

Thyroid hormone receptor- and stage-dependent transcriptome changes affect the initial period of Xenopus tropicalis tail regeneration

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: Dec. 31, 2024

Thyroid hormone (T3) has an inhibitory effect on tissue/organ regeneration. It is still elusive how T3 regulates this process. well established that the developmental effects of are primarily mediated through transcriptional regulation by thyroid receptors (TRs). Here we have taken advantage mutant tadpoles lacking both TRα and TRβ (TRDKO), only receptor genes in vertebrates, for RNA-seq analyses to investigate transcriptome changes underlying initiation tail regeneration, i.e., wound healing blastema formation, because crucial initial step determines extent functional regeneration later phase tissue regrowth.

Language: Английский