Recurrent innovation of protein-protein interactions in the Drosophila piRNA pathway DOI Creative Commons
Sebastian Riedelbauch, Sarah Masser,

Sandra Fasching

et al.

The EMBO Journal, Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

Abstract Despite being essential for fertility, genome-defense-pathway genes often evolve rapidly. However, little is known about the molecular basis of this adaptation. Here, we characterized evolution a protein interaction network within PIWI-interacting small RNA (piRNA) genome-defense pathway in Drosophila at unprecedented scale and evolutionary resolution. We uncovered pervasive rapid anchored heterochromatin 1 (HP1) paralog Rhino. Through cross-species high-throughput yeast-two-hybrid screening, identified three distinct trajectories across ~40 million years evolution. While several interactions are fully conserved, indicating functional conservation despite amino acid-sequence change, other preserved through coevolution were detected only between proteins or from closely related species. also species-restricted interactions, revealing insight into mechanistic diversity ongoing innovation piRNA production. In sum, our analyses reveal principles an adaptively evolving protein–protein network, support intermolecular as central mechanism adaptation protein-coding genes.

Language: Английский

Recurrent innovation of protein-protein interactions in the Drosophila piRNA pathway DOI Creative Commons
Sebastian Riedelbauch, Sarah Masser,

Sandra Fasching

et al.

The EMBO Journal, Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

Abstract Despite being essential for fertility, genome-defense-pathway genes often evolve rapidly. However, little is known about the molecular basis of this adaptation. Here, we characterized evolution a protein interaction network within PIWI-interacting small RNA (piRNA) genome-defense pathway in Drosophila at unprecedented scale and evolutionary resolution. We uncovered pervasive rapid anchored heterochromatin 1 (HP1) paralog Rhino. Through cross-species high-throughput yeast-two-hybrid screening, identified three distinct trajectories across ~40 million years evolution. While several interactions are fully conserved, indicating functional conservation despite amino acid-sequence change, other preserved through coevolution were detected only between proteins or from closely related species. also species-restricted interactions, revealing insight into mechanistic diversity ongoing innovation piRNA production. In sum, our analyses reveal principles an adaptively evolving protein–protein network, support intermolecular as central mechanism adaptation protein-coding genes.

Language: Английский

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