Transcriptome analysis of archived tumors by Visium, GeoMx DSP, and Chromium reveals patient heterogeneity DOI Creative Commons
Yixing Dong, Chiara Saglietti, Quentin Bayard

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: May 12, 2025

Abstract Recent advancements in probe-based, full-transcriptome technologies for FFPE tissues, such as Visium CytAssist, Chromium Flex, and GeoMx DSP, enable analysis of archival samples, facilitating the generation data from extensive cohorts. However, these methods can be labor-intensive costly, requiring informed selection based on research objectives. We compare tumor samples Breast, NSCLC DLBCL showing 1) good-quality, highly reproducible all methods; 2) containing cell mixtures despite marker-based preselection; 3) outperform discovering heterogeneity potential drug targets. recommend use high-throughput discovery projects, while manually more challenging platform with targeted regions remains valuable specialized questions.

Language: Английский

Transcriptome analysis of archived tumors by Visium, GeoMx DSP, and Chromium reveals patient heterogeneity DOI Creative Commons
Yixing Dong, Chiara Saglietti, Quentin Bayard

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: May 12, 2025

Abstract Recent advancements in probe-based, full-transcriptome technologies for FFPE tissues, such as Visium CytAssist, Chromium Flex, and GeoMx DSP, enable analysis of archival samples, facilitating the generation data from extensive cohorts. However, these methods can be labor-intensive costly, requiring informed selection based on research objectives. We compare tumor samples Breast, NSCLC DLBCL showing 1) good-quality, highly reproducible all methods; 2) containing cell mixtures despite marker-based preselection; 3) outperform discovering heterogeneity potential drug targets. recommend use high-throughput discovery projects, while manually more challenging platform with targeted regions remains valuable specialized questions.

Language: Английский

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