Engineering lipase TLL to improve its acid tolerance and its biosynthesis in Trichoderma reesei for biodiesel production from acidified oil DOI
Feng-Chao Wang, Yuqiang Li, Yali Wang

et al.

Bioresource Technology, Journal Year: 2024, Volume and Issue: 413, P. 131521 - 131521

Published: Sept. 24, 2024

Language: Английский

SubTuner leverages physics-based modeling to complement AI in enzyme engineering toward non-native substrates DOI
Qianzhen Shao,

Asher C. Hollenbeak,

Yaoyukun Jiang

et al.

Chem Catalysis, Journal Year: 2025, Volume and Issue: unknown, P. 101334 - 101334

Published: March 1, 2025

Language: Английский

Citations

1
htFuncLib: Designing libraries of active-site multipoint mutants for protein optimization DOI Creative Commons
Rosalie Lipsh‐Sokolik, Sarel J. Fleishman

Journal of Molecular Biology, Journal Year: 2025, Volume and Issue: unknown, P. 169011 - 169011

Published: Feb. 1, 2025

Language: Английский

Citations

0
Enhancement of thermostability and expression level of Rasamsonia emersonii lipase in Pichia pastoris and its application in biodiesel production in a continuous flow reactor DOI

Buqing Wang,

Xiaoman Zhou,

Yasen Wang

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 278, P. 134481 - 134481

Published: Aug. 9, 2024

Language: Английский

Citations

3
Cutting-edge Computational Approaches in Enzyme Design and Activity Enhancement DOI

Ruobin Sun,

Dan Wu, Pengcheng Chen

et al.

Biochemical Engineering Journal, Journal Year: 2024, Volume and Issue: 212, P. 109510 - 109510

Published: Sept. 26, 2024

Language: Английский

Citations

3
Insights on the role of blocking agent on the properties of the lipase from Thermomyces lanuginosus immobilized on heterofunctional support for hydroesterification reactions DOI Creative Commons

Felipe Cardoso Miranda,

Kaíque Souza Gonçalves Cordeiro Oliveira,

Paulo Waldir Tardioli

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 275, P. 133555 - 133555

Published: July 1, 2024

Here, we report a study of the effect blocking agent on properties lipase from Thermomyces lanuginosus (TLL) immobilized heterofunctional support (Purolite C18-ethylnediamina (EDA)- vinyl sulfone (VS)-TLL-blocking agent) in different reactions. The performance biocatalysts was compared to those standard hydrophobic C18-TLL) and commercial one (TLL-IM). nature (Cys, Gly Asp) altered enzyme features. TLL-IM always gave comparatively worse performance, with its specificity for oil being very Purolite biocatalysts. Under optimized conditions, C18-TLL yielded 97 % hydrolysis conversion after 4 h using water/waste cooking soybean (WCSO) mass ratio 4.3, biocatalyst load 6.5 wt% temperature 44.2 °C (without buffer or emulsification agent). In esterification reactions purified free fatty acids (FFAs) obtained WCSO, best TLL depended utilized alcohol: linear amyl alcohol preferred by C18-EDA-VS-TLL-Gly, while higher activity achieved utilizing isoamyl as nucleophile C18-EDA-VS-TLL-Cys, C18-EDA-VS-TLL-Asp IM-TLL catalysts. All results indicate influence step final

Language: Английский

Citations

2
Expanding the horizon of biodiesel production via enzyme engineering DOI

Siti nor Hasmah Ishak,

Raja Noor Zaliha Raja Abd Rahman,

Nor Hafizah Ahmad Kamarudin

et al.

International Journal of Green Energy, Journal Year: 2024, Volume and Issue: 21(14), P. 3367 - 3390

Published: June 18, 2024

Biodiesel generated from oils and fats composed of fatty acid alkyl esters is a potential substitution for fossil fuels, gaining attention as the demand sustainable energy grows. Enzymatic transesterification has emerged promising method biodiesel production due to low required, easy glycerol recovery compatibility with various feedstocks. This also offers high specificity operates under mild reaction conditions. However, limitations such enzyme cost, product yield, stability harsh conditions, have hindered progress biocatalysis. Protein engineering an important strategy overcome these limitations, particularly through rational design. Rational design approaches are advantageous in lipase engineering, accessible experimental tools existing knowledge structure function. Site-directed mutagenesis been applied improve stability, substrate specificity, methanol tolerance, catalytic efficiency by introducing molecular interactions like hydrogen bonds disulfide bridges well improved lid flexibility mutations at or swapping. targeted approach allows precise modification amino residues, optimizing better performance, which crucial efficient production.

Language: Английский

Citations

1
Engineering lipase TLL to improve its acid tolerance and its biosynthesis in Trichoderma reesei for biodiesel production from acidified oil DOI
Feng-Chao Wang, Yuqiang Li, Yali Wang

et al.

Bioresource Technology, Journal Year: 2024, Volume and Issue: 413, P. 131521 - 131521

Published: Sept. 24, 2024

Language: Английский

Citations

1