Ligninolytic peroxidases: A guide for their heterologous expression in E. coli and protocols to evaluate their activity on lignin
Methods in enzymology on CD-ROM/Methods in enzymology,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
Language: Английский
Biological pretreatment of lignocellulosic biomass as a sustainable option for biofuel production
Next Sustainability,
Journal Year:
2025,
Volume and Issue:
5, P. 100133 - 100133
Published: Jan. 1, 2025
Language: Английский
Saprotrophic Wood Decay Ability and Plant Cell Wall Degrading Enzyme System of the White Rot Fungus Crucibulum laeve: Secretome, Metabolome and Genome Investigations
Journal of Fungi,
Journal Year:
2024,
Volume and Issue:
11(1), P. 21 - 21
Published: Dec. 31, 2024
The
basidiomycete
Crucibulum
laeve
strain
LE-BIN1700
(Agaricales,
Nidulariaceae)
is
able
to
grow
on
agar
media
supplemented
with
individual
components
of
lignocellulose
such
as
lignin,
cellulose,
xylan,
xyloglucan,
arabinoxylan,
starch
and
pectin,
also
effectively
destroy
digest
birch,
alder
pine
sawdust.
C.
produces
a
unique
repertoire
proteins
for
the
saccharification
plant
biomass,
including
predominantly
oxidative
enzymes
laccases
(family
AA1_1
CAZymes),
GMC
oxidoreductases
AA3_2
FAD-oligosaccharide
oxidase
AA7
CAZymes)
lytic
polysaccharide
monooxygenases
LPMO
X325),
well
accompanying
acetyl
esterases
loosenine-like
expansins.
Metabolomic
analysis
revealed
that,
specifically,
monosaccharides
carboxylic
acids
were
key
low
molecular
metabolites
in
culture
liquids
experimental
conditions.
proportion
polyols
total
pool
identified
compounds
increased
sawdust-containing
media.
Multiple
copies
family
AA1_1,
AA3_2,
LPMOs
CAZyme
genes,
eight
genes
encoding
YvrE
superfamily
(COG3386),
which
includes
sugar
lactone
lactonases,
predicted
genome.
According
metabolic
pathway
analysis,
litter
saprotroph
can
catabolize
D-gluconic
D-galacturonic
acids,
possibly
other
aldonic
seems
confer
certain
ecological
advantages.
Language: Английский
Enhancing Manganese Peroxidase: Innovations in Genetic Modification, Screening Processes, and Sustainable Agricultural Applications
Lu He,
No information about this author
Mingchen Yan,
No information about this author
Muhammad Naeem
No information about this author
et al.
Journal of Agricultural and Food Chemistry,
Journal Year:
2024,
Volume and Issue:
72(47), P. 26040 - 26056
Published: Nov. 13, 2024
Manganese
peroxidase
(MnP),
a
vital
extracellular
enzyme
for
the
degradation
of
lignin
and
other
organic
pollutants,
has
demonstrated
immense
potential
agricultural
environmental
applications,
including
straw
pretreatment,
feed
fermentation,
mycotoxin
degradation,
water
treatment.
However,
current
research
remains
in
its
exploratory
phase,
with
naturally
sourced
MnP
unable
to
meet
industrial-scale
demands
no
mature
commercial
preparations
available
on
market.
This
comprehensive
review
innovatively
constructs
framework
research,
probing
into
molecular
conformation
catalytic
principles,
while
providing
an
overview
advancements
high-throughput
screening
Language: Английский