Utilization of the liquid one carbon feedstocks methanol and formate for acetogenic bioproduction of chemicals and fuels DOI Creative Commons
Eugenio Pettinato, Rémi Hocq, Stefan Pflügl

et al.

Bioresource Technology, Journal Year: 2025, Volume and Issue: unknown, P. 132643 - 132643

Published: May 1, 2025

The fight against climate change requires consideration of carbon as a critical parameter in production systems, with the ultimate aim creating truly sustainable circular economy. In this context, microbial bioproduction systems are promising route to renewably generate value-added chemicals and fuels. Methanol formate have recently gained interest one-carbon feedstocks, which can be produced sustainably from dioxide renewable energy, easy store transport readily dissolve aqueous solutions. Acetogenic bacteria strictly anaerobic microorganisms that grow autotrophically on molecular hydrogen or use methanol, formate, monoxide their sole energy sources via Wood-Ljungdahl pathway, most energetically efficient fixation pathway known date. Here, variants physiology selection methylotrophic formatotrophic acetogens, emphasize recent advancements bioprocessing respect quantification acetogen metabolism methanol well research aiming at establishing novel bioprocesses reviewed. Additionally, tools available for physiological metabolic studies genetic engineering discussed. Finally, features constraints govern bioenergetics stoichiometry during growth reviewed, future perspectives field high energetic efficiency acetogens convert into products renders them highly attractive platform hosts

Language: Английский

A megatransposon drives the adaptation of Thermoanaerobacter kivui to carbon monoxide DOI Creative Commons
Rémi Hocq, Josef Horvath,

Maja Stumptner

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: May 6, 2025

Abstract Acetogens are promising industrial biocatalysts for upgrading syngas, a gas mixture containing CO, H 2 and CO into fuels chemicals. However, severely inhibits growth of many acetogens, often requiring extensive adaptation to enable efficient conversion (carboxydotrophy). Here, we adapt the thermophilic acetogen Thermoanaerobacter kivui use as sole carbon energy source. Isolate CO-1 exhibits rapid on syngas (co-utilizing ) in batch continuous cultures (µ max ~ 0.25 h −1 ). The carboxydotrophic phenotype is attributed mobilization CO-dependent megatransposon originating from locus responsible autotrophy T. . Transcriptomics reveal crucial role redox balance plays during growth. These insights exploited rationally engineer grow CO. Collectively, our work elucidates primary mechanism acquisition carboxydotrophy acetogens showcases how transposons can orchestrate evolution.

Language: Английский

Citations

0
Utilization of the liquid one carbon feedstocks methanol and formate for acetogenic bioproduction of chemicals and fuels DOI Creative Commons
Eugenio Pettinato, Rémi Hocq, Stefan Pflügl

et al.

Bioresource Technology, Journal Year: 2025, Volume and Issue: unknown, P. 132643 - 132643

Published: May 1, 2025

The fight against climate change requires consideration of carbon as a critical parameter in production systems, with the ultimate aim creating truly sustainable circular economy. In this context, microbial bioproduction systems are promising route to renewably generate value-added chemicals and fuels. Methanol formate have recently gained interest one-carbon feedstocks, which can be produced sustainably from dioxide renewable energy, easy store transport readily dissolve aqueous solutions. Acetogenic bacteria strictly anaerobic microorganisms that grow autotrophically on molecular hydrogen or use methanol, formate, monoxide their sole energy sources via Wood-Ljungdahl pathway, most energetically efficient fixation pathway known date. Here, variants physiology selection methylotrophic formatotrophic acetogens, emphasize recent advancements bioprocessing respect quantification acetogen metabolism methanol well research aiming at establishing novel bioprocesses reviewed. Additionally, tools available for physiological metabolic studies genetic engineering discussed. Finally, features constraints govern bioenergetics stoichiometry during growth reviewed, future perspectives field high energetic efficiency acetogens convert into products renders them highly attractive platform hosts

Language: Английский

Citations

0