Hepatozoon (Eucoccidiorida: Hepatozoidae) in wild mammals of the Americas: a systematic review

Parasites & Vectors, Journal Year: 2024, Volume and Issue: 17(1)

Published: March 6, 2024

Abstract Background The study of parasites provides insight into intricate ecological relationships in ecosystem dynamics, food web structures, and evolution on multiple scales. Hepatozoon (Eucoccidiorida: Hepatozoidae) is a genus protozoan hemoparasites with heteroxenous life cycles that switch infections between vertebrates blood-feeding invertebrates. most comprehensive review the was published 26 years ago, currently there are no harmonized data epizootiology, diagnostics, genotyping methods, evolutionary relationships, genetic diversity Americas. Methods Here, we provide based PRISMA method regarding wild mammals within American continent, order to generate framework for future research. Results 11 out 35 countries Americas (31.4%) had , Carnivora Rodentia orders having characterizations. Bats, ungulates, shrews were least affected groups. While americanum H. -like, canis didelphydis felis milleri griseisciuri procyonis correspond identified species, plethora genospecies pending formal description combining morphology genetics. Most vectors unknown, but some flea, mite, tick species have been confirmed. detection has relied mostly conventional polymerase chain reaction (PCR), implementation specific real time PCR needs be employed improve its diagnosis animals future. From perspective, V4 region 18S rRNA gene widely sequenced identification animals. However, mitochondrial apicoplast markers should also targeted truly determine different genus. A phylogenetic analysis herein retrieved ribosomal DNA (rDNA) sequences showed two main clades : Clade I associated small mammals, birds, herpetozoa, II Carnivora. topology tree reflected haplotype network. Conclusions Finally, our emphasizes as potential disease agent threatened role canids spreaders Graphical Abstract

Language: Английский

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Phylogenetic analysis and haplotype networking of Hepatozoon felis infecting wild animals in Gir National Park, Gujarat, India

Parasitology Research, Journal Year: 2024, Volume and Issue: 123(1)

Published: Jan. 1, 2024

Language: Английский

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Genetic and Haplotype Diversity of Hoplolaimus (Nematoda: Hoplolaimidae) Through Analysis of COI of mtDNA

Horticulturae, Journal Year: 2025, Volume and Issue: 11(2), P. 113 - 113

Published: Jan. 22, 2025

Lance nematodes (Hoplolaimus spp.) feed on the roots of various plants, including key horticultural products. An investigation genetic diversity and structure six Hoplolaimus species, utilizing cytochrome c oxidase I (COI) mtDNA gene, was based 174 sequences available NCBI. Based COI mtDNA, haplotype analysis revealed 44 haplotypes. Nucleotide low among all species Hoplolaimus, except for H. magnistylus (π = 0.04915) stephanus 0.06746). In contrast, (Hd) high, especially (Hd 0.89) pararobustus 0.90). Phylogenetic grouped populations into eight clades, result showed that placed in three different which high variability supported by network. Neutrality tests mismatch distribution hypothesis a constant population with no expansion concaudajuvencus (Tajima (D) −0.84971) columbus −0.87674). conclusion, neutral evolution amongst species. The present study provides better insight toward delimitation managing this plant-parasitic nematode crops.

Language: Английский

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Integrative taxonomy in helminth analysis: protocols and limitations in the twenty-first century

Parasites & Vectors, Journal Year: 2025, Volume and Issue: 18(1)

Published: March 5, 2025

The term integrative taxonomy was coined in 2005 for the identification of microorganisms using morphological, molecular, pathological and ecological components. Since then, more than 200 scientific articles have been published this helminthology field different geographical regions world, leading to accurate identifications specimens expanding known biodiversity by describing novel species. Importantly, responsible use approach has led balanced results which molecular data do not overshadow or information. By having perspectives, new taxonomic situations arrived including definition cryptic diversity, species complex genotypes. In present work, we main procedures principles conducting an study parasitic helminth collection preservation specimens, fixation staining worms light microscopy scanning electron microscopy, histopathological, DNA analysis. This guide is aimed at researchers their endeavor analyze parasites collected from wild domestic animals with examples provided, but protocols presented herein may be extrapolated analysis helminths other hosts (e.g. human fish). One important aspect that recommendations derive authors' experiences, will hopefully reader.

Language: Английский

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Perspectives of Population Genetics in the Genetic Improvement and Biodiversity Conservation of Fish Species

IntechOpen eBooks, Journal Year: 2025, Volume and Issue: unknown

Published: March 25, 2025

This chapter explores the applications of population genetics in genetic improvement and biodiversity conservation fish species. It traces evolution genetics, from earliest measurements variation at allozyme loci to current era genomics, highlighting technological advances that have revolutionized field. The emphasizes importance understanding both historical recent processes shaped patterns diversity, particularly face declines driven by anthropogenic activities climate change. Various molecular techniques used assess diversity are analyzed, including traditional markers such as allozymes DNA-based markers, amplified fragment length polymorphisms (AFLP), mitochondrial DNA (mtDNA), microsatellites. We discuss advantages disadvantages each method, emphasizing their application structure analysis, gene flow assessment, reproductive strategy studies. Additionally, addresses use these marker-assisted selection enhance productive traits commercially important primary objective is highlight how concepts, flow, drift, natural selection, can be applied improve Furthermore, for health sustainability aquatic populations, captivity wild, underscored.

Language: Английский

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Fatal babesiosis in a free-ranging Iberian wolf co-infected with Hepatozoon canis and Leishmania infantum: Epidemiological Implications for the Cantabrian Wolf Population

International Journal for Parasitology Parasites and Wildlife, Journal Year: 2025, Volume and Issue: unknown, P. 101080 - 101080

Published: May 1, 2025

Language: Английский

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Analysis of genetic diversity and population structure of Babesia gibsoni

Frontiers in Veterinary Science, Journal Year: 2023, Volume and Issue: 10

Published: March 23, 2023

Babesia gibsoni is a tick-borne apicomplexan protozoan causing canine babesiosis. This parasite has diploid sexual reproduction in ticks, during which genetic exchanges can occur leading to increased diversity, an important factor adapting environmental changes. Exploring the variation of B. population provide foundation for understanding patterns disease transmission and developing babesiosis control strategies. Partial 18S rRNA fragment sequences were obtained from 11 isolates collected different regions China 117 publicly available 12 geographical areas including China. The variation, demographic expansion structure examined. A total 34 haplotypes identified among populations. Analysis molecular variance, pairwise Fst analysis showed that high within populations, low differentiation obvious mixture haplotype apparent single continent, but higher was detected across continents. Neutrality tests implied populations had experienced extension. These findings will contribute understand genetics evolution be useful formulating effective management strategies prevent this parasite.

Language: Английский

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Geographical isolation and hyperendemicity of Hepatozoon felis: Epidemiological scenario in Skopelos, Greece, and phylogenetic analysis

Current Research in Parasitology and Vector-Borne Diseases, Journal Year: 2024, Volume and Issue: 6, P. 100202 - 100202

Published: Jan. 1, 2024

Feline hepatozoonosis is a vector-borne disease caused by different species of the genus

Language: Английский

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Hepatozoon spp. infection in wild canids in the eastern United States

Parasites & Vectors, Journal Year: 2023, Volume and Issue: 16(1)

Published: Oct. 19, 2023

Hepatozoon spp. are apicomplexan parasites known to cause musculoskeletal disease in a variety of animals. Two species infect wild and domestic canids the US: canis H. americanum.In this study, blood, heart, and/or spleen samples were collected from 278 (180 coyotes, 93 red foxes, 5 gray foxes) eastern US tested via PCR for Hepatozoon. Histology slides heart skeletal muscle assessed cysts associated inflammation when fresh tissue was available (n = 96).Hepatozoon found 24.2% (59/278) individuals, with 14.0% (34/278) americanum 10.7% (26/278). One coyote positive both americanum. Foxes more likely be than coyotes (23% 7% respectively, P 0.0008), while only Of eight sampled states, present six (Louisiana, North Carolina, Pennsylvania, South Tennessee, Virginia) two southern states (South Carolina Louisiana). Infection status positively correlated myositis myocarditis, or 83% (5/6) americanum-positive coyotes.This survey showed moderate prevalence where parasite previously unrecorded including Pennsylvania.

Language: Английский

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Molecular Survey and Genetic Characteristics of Vector-Borne Pathogens in Domestic Dogs from Four Regions of China

Animals, Journal Year: 2023, Volume and Issue: 13(11), P. 1867 - 1867

Published: June 3, 2023

Canine vector-borne diseases are widely distributed around the world. They transmitted by arthropods, and many seriously threaten health of animals humans. In China, our knowledge Ehrlichia, Hepatozoon, Mycoplasma species circulating in dogs is still poorly understood. Therefore, aim this study was to understand prevalence genetic characteristics canine Ehrlichia spp., Hepatozoon spp. Chongqing (southwest), Fujian (southeast), Shandong Hubei (central) Provinces China. Blood samples from healthy pet were processed detect DNA with PCR. Haplotype phylogenetic analyses performed on 18S rRNA sequences. Among 306 dogs, no or detected, whereas one sp. detected 10 (3.27%) animals. Only canis identified endemic (2.46%) (8.77%). A haplotype analysis eight haplotypes among H. isolates. showed that isolates clustered into four clades, together different countries hosts, forming a large group clearly separate other species. These findings provided new information epidemiological China will be helpful development efficient measures safeguard well-being companion their owners.

Language: Английский

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