Cellular Senescence Contributes to Colonic Barrier Integrity Impairment Induced by Toxoplasma gondii Infection

Inflammation, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 18, 2025

Toxoplasma gondii (T. gondii) induces gut barrier integrity impairment, which is crucial to the establishment of long-term infection in hosts. Cellular senescence an imperative event that drives disease progression. Several studies have indicated T. oxidative stress and cell cycle blockade tissues hosts, suggesting cellular induced by parasite. Here, we explored whether involved gondii-mediated colonic damage mice. C57BL/6J mice were infected with 10 cysts gondii. Senolytic therapy (dasatinib quercetin, DQ, a combination for reducing senescent cells) was given oral gavage 4 weeks post-infection. Alcian blue staining, immunofluorescence, western blot, quantitative PCR (qPCR), enzyme-linked immunosorbent assay (ELISA) employed evaluate thickness mucus layer, expression profiles genes proteins related tight junction function tissues, levels serum lipopolysaccharides (LPS), respectively. gondii-infected exhibited deteriorated secreted mucus, shortened length, decreased zonula occludens-1 (ZO-1) occludin colon, accompanied elevated LPS. Moreover, upregulated senescence-related markers (p16INK4A, p21CIP1) while inhibiting Lamin B1 expression. In addition, senescence-associated secretory phenotypes (SASPs), including IL-1β, TNF-α, IL-6, MMP9 CXCL10, Notably, DQ administration, significantly ameliorated pathological alterations infection. This study uncovers first time contributes chronic Importantly, provide evidence senolytic exerts therapeutic effect on intestinal lesions.

Language: Английский

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The Legalome: Microbiology, Omics and Criminal Justice

Microbial Biotechnology, Journal Year: 2025, Volume and Issue: 18(3)

Published: March 1, 2025

ABSTRACT Advances in neuromicrobiology and related omics technologies have reinforced the idea that unseen microbes play critical roles human cognition behaviour. Included this research is evidence indicating gut microbes, through direct indirect pathways, can influence aggression, anger, irritability antisocial Moreover, manufacture chemicals are known to compromise cognition. For example, recent court decisions United States Europe acknowledge produce high levels of ethanol, without consumption alcohol by defendants. The dismissal driving while intoxicated charges these cases—so‐called auto‐brewery syndrome—highlights way which microbiome knowledge will enhance precision, objectivity fairness our legal systems. Here opinion essay, we introduce concept ‘legalome’—the application science forensic psychiatry criminal law. We argue rapid pace microbial discoveries, including those challenge ideas free moral responsibility, necessitate a reconsideration traditional doctrines justifications retributive punishment. implications extend beyond courtroom, challenging us reconsider how environmental factors—from diet socioeconomic conditions—might shape preventative rehabilitative efforts their effects on microbiome.

Language: Английский

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Interaction between the liver transcriptome and gut microbiota in mice during Toxoplasma gondii infection as identified by integrated transcriptomic and microbiome analysis

BMC Microbiology, Journal Year: 2025, Volume and Issue: 25(1)

Published: March 14, 2025

Toxoplasma gondii is a single-cell parasite capable of infecting both humans and variety animal species. Although T. infection known to adversely affect the liver gut microbiota, precise interplay between microbiome transcriptome in infected mice remains largely unknown. In this study, we artificially induced acute chronic stages BALB/c via oral low doses (n = 10) PRU (Type II) bradyzoites. Then, performed fecal 16S rRNA gene amplicon sequencing RNA investigate composition microbiota expression profiles long non-coding RNAs (lncRNAs), circular (circRNAs), microRNAs (miRNAs), messenger (mRNAs) livers with at different infection. Analysis revealed dynamic alterations following over course cycle. Notably, observed significant increase abundance Enterobacteriaceae during stage infection, while Lactobacteriaceae was elevated stage. Liver analysis identified numerous differentially expressed (DE) mRNAs potentially involved mediating immune responses inflammation by gondii. During several pro-inflammatory genes, including Lpin1, Usp2, Pim3, Il6ra were significantly up-regulated liver. Among these, Lpin1 may be closely associated development overgrowth. Conversely, some anti-inflammatory such as Dmbt1, Ddit4, exclusively Gene ontology (GO) enrichment further stage-specific features functionality. Specifically, pathways enriched. Interestingly, related regulation, 'defense response Gram-negative bacterium', 'antimicrobial humoral mediated antimicrobial peptide', response' Additionally, competing endogenous (CeRNAs) networks that DElncRNAs DEcircRNAs competitively regulated DEmiRNA mmu-miR-690, which targets Nr1d1 gene. These findings provide insights into complex summary, our results highlight intricate interaction key genes

Language: Английский

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Gut microbiota-derived butyrate alleviates the impairment of mice intestinal integrity caused by toxoplasma gondii infection

Life Sciences, Journal Year: 2025, Volume and Issue: unknown, P. 123709 - 123709

Published: May 1, 2025

Language: Английский

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The Intestinal Barrier Protective Effect of Indole Aldehyde Derivatives on Acute Toxoplasma gondii Infection

Molecules, Journal Year: 2024, Volume and Issue: 29(21), P. 5024 - 5024

Published: Oct. 24, 2024

Toxoplasmosis, a zoonotic infection caused by

Language: Английский

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