IL-6 Production Through Repression of UBASH3A Gene via Epigenetic Dysregulation of Super-Enhancer in CD4+T Cells in Rheumatoid Arthritis DOI Creative Commons
Kaoru Yamagata, Shingo Nakayamada, Tong Zhang

et al.

Research Square (Research Square), Journal Year: 2022, Volume and Issue: unknown

Published: Feb. 24, 2022

Abstract Background. UBASH3A as a negative regulator of T-cell receptors (TCRs) signaling is susceptible factor for rheumatoid arthritis (RA) patients who exhibit immune dysfunction. We studied expression in CD4 + T cells from healthy donors and RA patients, the involvement super-enhancer (SE) control expression, contribution to proinflammatory cytokine production. Methods. The mRNA protein levels were examined by quantitative PCR Western blotting, respectively. Locked nucleic acid was administered inhibit enhancer RNA (eRNA) expression. Factors recruited loci displaying SE architecture chromatin immunoprecipitation. transfected with plasmids, measured cytometric bead array. Results. lower than those donors. reduced eRNA_1 eRNA_3 knockdown. In BTB CNC homology 2 (BACH2), silencing transcription factor, accumulated at cells, whereas SE-defining mediator complex subunit 1 (MED1)/bromodomain 4 (BRD4), did not. However, opposite phenomena observed Although stimulation TCRs expressed on led interleukin (IL)-6 production, over-expression significantly inhibited its Conclusions. Transcription suppressed via epigenetic regulation patients. Decreased lead excessive activation TCR signaling, resulting enhanced production IL-6.

Language: Английский

A novel long noncoding RNA ENST00000597482 serves as a potential biomarker for disease activity and diagnosis of systemic lupus erythematosus DOI
Cuicui Wang, Shiwen Yuan, Yanting Zeng

et al.

Lupus, Journal Year: 2024, Volume and Issue: 33(10), P. 1089 - 1099

Published: July 22, 2024

Emerging evidence indicate that long noncoding RNAs (lncRNAs) may play an important role in the pathogenesis of systemic lupus erythematosus (SLE) however, contribution lncRNAs to SLE remains largely unclear. Our study aimed explore lncRNA expression profiles peripheral blood mononuclear cells (PBMCs) from patients.

Language: Английский

Citations

1
FOSL1-mediated LINC01566 negatively regulates CD4+ T-cell activation in myasthenia gravis DOI Creative Commons
Lifang Li, D. Li,

Jingnan Jin

et al.

Journal of Neuroinflammation, Journal Year: 2024, Volume and Issue: 21(1)

Published: Aug. 7, 2024

Myasthenia gravis (MG) is an autoimmune disease characterized by pathogenic antibodies that target structures of the neuromuscular junction. The evidence suggests regulation long noncoding RNAs (lncRNAs) mediated transcription factors (TFs) plays a key role in pathophysiology MG. Nevertheless, detailed molecular mechanisms lncRNAs MG remain largely undetermined.

Language: Английский

Citations

1
Non-Coding RNAs in Myasthenia Gravis: From Immune Regulation to Personalized Medicine DOI Creative Commons
Nicola Iacomino, Maria Cristina Tarasco,

Alessia Berni

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(18), P. 1550 - 1550

Published: Sept. 14, 2024

Myasthenia gravis (MG) is an antibody-mediated autoimmune disorder characterized by altered neuromuscular transmission, which causes weakness and fatigability in the skeletal muscles. The etiology of MG complex, being associated with multiple genetic environmental factors. Over recent years, progress has been made understanding immunological alterations implicated disease, but exact pathogenesis still needs to be elucidated. A pathogenic interplay between innate immunity autoimmunity contributes intra-thymic development. Epigenetic changes are critically involved both adaptive immune response regulation. They can act as (i) pathological factors besides predisposition (ii) co-factors contributing disease phenotypes or patient-specific course/outcomes. This article reviews role non-coding RNAs (ncRNAs) epigenetic MG. Particular attention dedicated microRNAs (miRNAs), whose expression patients' thymuses circulating blood. long ncRNA (lncRNA) contribution MG, although not fully yet, also discussed. By summarizing most fast-growing findings on ncRNAs we highlight therapeutic potential these molecules for achieving regulation their value biomarkers development personalized medicine approaches improve care.

Language: Английский

Citations

1
Study on the Relationship between lncRNA Gene Polymorphism and Systemic Lupus Erythematosus DOI Open Access
Xue Li Guan, Dan Liu, Yuling Xing

et al.

Computational and Mathematical Methods in Medicine, Journal Year: 2022, Volume and Issue: 2022, P. 1 - 7

Published: Feb. 27, 2022

Systemic lupus erythematosus (SLE) is an autoimmune disease that can cause damage to multiple systems of the body. A number studies have shown long-chain noncoding RNA (lncRNA) participate in occurrence and development a variety diseases. This study aimed at detecting expression levels 5 lncRNAs SLE patients healthy controls exploring relationship between clinical symptoms laboratory indicators.The design type this case-control study. total 76 71 were included first phase Real-time fluorescence quantitative polymerase chain reaction was used detect level kinds including lnc7514, lnc0640, lncagf, nc3643, lnc5150 PBMCs two groups patients; case group control analyzed. We analyzed differences groups, explored association with manifestations characteristics. SPSS23.0 analyze gene polymorphism results; statistical analysis test α = 0.05.The lnc0640 patient higher than (Z -3.56, P 0.03). However, lower -7.16, < 0.001). lnc3643 pleurisy without -2.44, 0.02). Low observed rash -2.75, 0.013). expressed compared those -2.42, The above statistically significant. Association lncRNA found or (both 0.05); results negative correlation lnc3643, activity score (SLEDAI-2K), blood sink (ESR), C-reactive protein (CRP) (all 0.05).lnc0640 overexpressed controls. negatively correlated SLEDAI, associated arthritis, rash, pleuritis.

Language: Английский

Citations

3
Comprehensive Analysis of lncRNA Expression Profile and the Potential Role of ENST00000604491 in Graves’ Disease DOI Creative Commons
Yingzhao Liu,

Junli Zou,

Juan Xu

et al.

Journal of Immunology Research, Journal Year: 2022, Volume and Issue: 2022, P. 1 - 13

Published: April 25, 2022

Background. Graves’ disease (GD) is one of the most common autoimmune diseases worldwide and develops in 20 to 50 cases per 100,000 persons annually. Long noncoding RNAs (lncRNAs) are widely expressed multiple human have pivotal functions gene regulation. This study aimed at determining lncRNA profile peripheral blood mononuclear cells (PBMCs) from GD patients investigating role ENST00000604491 GD. Methods. A total 31 32 normal controls were enrolled study. Next-generation sequencing was performed identify dysregulated lncRNAs PBMCs 5 controls, 26 27 used verify selected lncRNAs. The relative expression verified lncRNAs, forkhead box P1 (FOXP1), IKAROS family zinc finger 3 (IKZF3) these samples detected by quantitative real-time PCR. potential biomarker value assessed using receiver operating characteristic (ROC) curve analysis. Results. 37,683 indicated, which significantly upregulated 83 remarkably downregulated compared with healthy subjects. Gene Ontology Kyoto Encyclopedia Genes Genomes pathway analyses showed that abnormally mainly enriched immune system-related signalling pathways. Among negatively correlated serum levels thyroid-stimulating hormone receptor antibodies (TRAb) patients. Further studies confirmed decreased FOXP1 inversely TRAb Moreover, there a notably positive correlation between transcript area under ROC up 0.74 (95% confidence interval: 0.60-0.87, p < 0.01 ), sensitivity specificity 53.85% 88.89%, respectively. Conclusion. present identifies as attenuated patients, may contribute pathogenesis regulating represent for

Language: Английский

Citations

3
Long non-coding RNAs: The modulators of innate and adaptive immune cells DOI Creative Commons

Aseel Riyadh Luaibi,

Montaha Al-Saffar,

Abduladheem Turki Jalil

et al.

Pathology - Research and Practice, Journal Year: 2022, Volume and Issue: 241, P. 154295 - 154295

Published: Dec. 30, 2022

Before very sensitive current genomics platforms were discovered, long non-coding RNAs (lncRNAs) as controllers of gene expression, thought to be accumulated genetic garbage. The past few years have seen a lot interest in large classification transcripts with an indeterminate length more than 200 nucleotides [1]. lncRNAs' association immunity and disease progression has been revealed by growing body experimental research. Only limited subset lncRNAs, however, solid proof their role. It is also clear that various immune cells express lncRNAs differently. In this review, we concentrated on the role lncRNA expression regulation cell function response pathological conditions macrophages, dendritic cells, natural killer (NK) neutrophils, Myeloid-derived suppressor (MDSCs), T B cells. innate adaptive systems may significantly regulated according emerging To discover possible therapeutic targets for therapy different diseases, it helpful better realization molecular mechanisms beyond response. Therefore, crucial investigate comprehend its significance system.

Language: Английский

Citations

3
Mechanisms and functions of lncRNAs linked to autoimmune disease risk alleles DOI

Ruxiao Tian,

Sankar Ghosh

Advances in immunology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 15

Published: Jan. 1, 2024

Language: Английский

Citations

0
Role of regulatory T cells in pathogenesis and therapeutics of systemic lupus erythematosus DOI
William de Jesús Ríos-Ríos, Honorio Torres‐Aguilar

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 79 - 94

Published: Jan. 1, 2024

Language: Английский

Citations

0
Pathological role of LncRNAs in immune-related disease via regulation of T regulatory cells DOI
Sulieman Ibraheem Shelash Al-Hawary, Khadisha Kashikova,

E Ioffe

et al.

Pathology - Research and Practice, Journal Year: 2023, Volume and Issue: 249, P. 154709 - 154709

Published: July 22, 2023

Language: Английский

Citations

1
lncRNA involvement in immune-related diseases - from SNP association to implication in pathogenesis and therapeutic potential DOI Open Access
Leire Bergara-Muguruza, Ainara Castellanos–Rubio, Izortze Santín

et al.

Journal of Translational Genetics and Genomics, Journal Year: 2023, Volume and Issue: 7(4), P. 213 - 229

Published: Nov. 27, 2023

Development of new high throughput array-based techniques and, more recently, next-generation sequencing (NGS) technologies have revolutionized our capability to accurately characterize single nucleotide polymorphisms (SNPs) throughout the genome. These advances facilitated large-scale genome-wide association studies (GWAS), which served as fundamental elements in establishing links between SNPs and susceptibility several complex diseases, including those related immune system. Nevertheless, molecular mechanisms underlying development most these disorders are still poorly defined. Decoding functionality becomes increasingly challenging due predominant presence risk variants non-coding regions Among them, long RNAs (lncRNAs) enriched disease-associated SNPs. lncRNAs involved governing control gene expression both during transcription at post-transcriptional level. The existence within sequences has potential alter their expression, structure, or function. This, turn, can influence regulatory roles consequently contribute onset progression various diseases. In this review, we describe implication located different immune-related diseases highlight molecules emerging RNA-based therapies.

Language: Английский

Citations

1