Transcriptional landscape of cotton roots in response to salt stress at single-cell resolution
Plant Communications,
Journal Year:
2023,
Volume and Issue:
5(2), P. 100740 - 100740
Published: Oct. 29, 2023
Increasing
soil
salinization
has
led
to
severe
losses
of
plant
yield
and
quality.
Thus,
it
is
urgent
investigate
the
molecular
mechanism
salt
stress
response.
In
this
study,
we
took
systematically
analyzed
cotton
root
response
by
single-cell
transcriptomics
technology;
56,281
high-quality
cells
were
totally
obtained
from
5-days-old
lateral
tips
Gossypium
arboreum
under
natural
growth
different
salt-treatment
conditions.
Ten
cell
types
with
an
array
novel
marker
genes
synthetically
identified
confirmed
in
situ
RNA
hybridization,
some
specific-type
pesudotime
analysis
also
pointed
out
their
potential
differentiation
trajectory.
The
prominent
changes
numbers
responding
observed
on
outer
epidermal
inner
endodermic
cells,
which
significantly
enriched
stress,
amide
biosynthetic
process,
glutathione
metabolism,
glycolysis/gluconeogenesis.
Other
functional
aggregations
concentrated
plant-type
primary
wall
biogenesis,
defense
response,
phenylpropanoid
biosynthesis
metabolic
pathways
analyzing
abundant
differentially
expressed
(DEGs)
multiple
comparisons.
Some
candidate
DEGs
related
transcription
factors
hormones
identified,
function
Ga03G2153,
annotated
auxin-responsive
GH3.6,
was
using
virus-induced
gene
silencing
(VIGS).
GaGH3.6-silenced
plants
presented
stress-susceptive
phenotype,
suffered
more
serious
oxidative
damages
detecting
physiological
biochemical
indexes,
indicating
that
GaGH3.6
might
participate
tolerance
through
regulating
oxidation-reduction
process.
For
first
time,
a
transcriptional
atlas
roots
characterized
at
resolution,
explored
cellular
heterogeneityand
trajectory,
providing
valuable
insights
into
underlying
plants.
Language: Английский
Physiological and lipidomic response of exogenous choline chloride alleviating salt stress injury in Kentucky bluegrass (Poa pratensis)
Zhi-Fang Zuo,
No information about this author
Yan Li,
No information about this author
Xin-Feng Mi
No information about this author
et al.
Frontiers in Plant Science,
Journal Year:
2023,
Volume and Issue:
14
Published: Aug. 31, 2023
Introduction
Choline
participates
in
plant
stress
tolerance
through
glycine
betaine
(GB)
and
phospholipid
metabolism.
As
a
salt-sensitive
turfgrass
species,
Kentucky
bluegrass
(
Poa
pratensis
)
is
the
main
species
cool-season
areas.
Methods
To
improve
salinity
investigate
effects
of
choline
on
physiological
lipidomic
responses
plants
under
conditions,
exogenous
chloride
was
applied
to
exposed
salt
stress.
Results
From
indicators,
could
alleviate
injury
bluegrass.
Lipid
analysis
showed
that
salt-stress
conditions
remodeled
content
phospholipids,
glycolipids,
lysophospholipids.
Monogalactosyl
diacylglycerol,
digalactosyl
phosphatidylcholine,
phosphatidylethanolamine,
phosphatidylserine,
lysophosphatidylcholine
were
increased
phosphatidic
acid
decreased
after
treatment.
Plant
leaf
increased,
but
GB
not
detected
treatment
nonstress
or
conditions.
Discussion
synthesis
pathway
related
genes
no
clear
change
treatment,
whereas
cytidyldiphosphate‐choline
(CDP‐choline)
upregulated
by
These
results
reveal
lipid
remodeling
metabolism
plays
an
important
role
mechanism
Furthermore,
lipids
selected
this
study
serve
as
biomarkers
for
further
improvement
grass
species.
Language: Английский
Feasibility of Nano-Urea and PGPR on Salt Stress Amelioration in Reshmi Amaranth (Amaranthus tricolor): Stress Markers and Enzymatic Response
Horticulturae,
Journal Year:
2025,
Volume and Issue:
11(3), P. 280 - 280
Published: March 5, 2025
The
present
experiment
aimed
to
examine
the
impact
of
nano
urea
(NU)
and
plant
growth-promoting
rhizobacteria
(PGPR)
on
Reshmi
amaranth
(Amaranthus
tricolor)
growth
under
salt
stress.
Experiments
were
conducted
using
six
different
combinations
NaCl,
NU,
PGPR
for
35
days
greenhouse
conditions.
results
showed
that
salinity
stress
significantly
(p
<
0.05)
reduced
parameters,
including
shoot
height,
root
length,
fresh
weight,
leaf
area.
However,
application
NU
PGPR,
both
individually
in
combination,
enhanced
physiological
resilience
saline
+
treatment
yielded
best
improvements,
with
a
height
42.25
cm,
length
34.79
weight
61.69
g,
indicating
synergistic
effect.
Biochemical
analysis
NaCl
lowered
chlorophyll
(0.25
mg/g
fwt.)
carotenoids
(60.17
µg/100
g)
disrupted
ionic
homeostasis
by
increasing
Na⁺
accumulation
while
reducing
K+
Ca2+
uptake.
combined
restored
balance,
58.12
mg
K⁺
levels
115.25
78.70
mg,
respectively.
Stress
markers
such
as
malondialdehyde
(MDA)
proline
also
significant
reductions,
antioxidant
enzyme
activities
stabilized
application.
Thus,
this
study
indicated
mitigate
salt-induced
improving
nutrient
assimilation,
promoting
osmotic
regulation,
enhancing
antioxidative
defenses
amaranth.
Language: Английский
NaCl elicitation enhances metabolite accumulation and stress resilience in Inula crithmoides L. shoot cultures: implications for its nutritional and medicinal value
Plant Cell Tissue and Organ Culture (PCTOC),
Journal Year:
2024,
Volume and Issue:
157(1)
Published: April 1, 2024
Abstract
This
study
explored
the
impact
of
sodium
chloride
(NaCl)
elicitation
on
accumulation
primary
and
secondary
metabolites
oxidative
stress
responses
Inula
crithmoides
L.
(golden
samphire)
in
vitro
shoot
cultures.
Elicitation
involved
applying
different
concentrations
NaCl
(0,
50,
100,
200
mM)
for
4
weeks.
was
followed
by
assessing
its
plant
growth,
physiological
parameters
(pigments,
hydrogen
peroxide
content,
total
soluble
sugars
proteins,
proline),
metabolism
(phenylalanine
ammonia-lyase
activity,
shikimic
acid,
phenolics,
flavonoids,
hydroxycinnamic
acids)
shoots.
The
extracts
were
also
analysed
using
high-performance
liquid
chromatography
(HPLC).
did
not
affect
growth
but
increased
functions
such
as
photosynthesis
management
under
moderate
salinity
levels.
In
addition,
treatments
synthesis
particularly
proline,
well
bioactive
phenolics
gentisic
chlorogenic
4-hydroxybenzoic
luteolin-7-O-glucoside,
naringenin-7-O-glucoside.
golden
samphire
cultures
offers
a
significant
method
enhancing
production
important
nutritional
compounds.
underscores
species’
potential
cultivation
saline
environments
provides
valuable
prospects
utilization
health
nutrition
sectors.
Language: Английский
Na+ exclusion and proline accumulation in Urochloa spp. cultivars
Pesquisa Agropecuária Brasileira,
Journal Year:
2023,
Volume and Issue:
58
Published: Jan. 1, 2023
Abstract
The
objective
of
this
work
was
to
evaluate
the
effect
salinity
on
Na+
and
K+
distribution
proline
content
in
Urochloa
brizantha
decumbens
grass
cultivars.
Plants
were
grown
nutrient
solution
subjected
salt
stress
for
nine
days.
Shoot
root
lengths
proline,
total
chlorophyll,
K+,
contents
determined.
Salt
reduced
shoot
length
cultivar
Marandu
but
not
Xaraés
Basilisk.
Total
chlorophyll
affected
by
U.
cultivars,
increased
Basilisk
decumbens.
Salt-induced
accumulation
observed
all
with
a
higher
increase
Marandu.
leaf
blades
cultivars
under
conditions.
'Basilisk'
showed
exclusion
tissues,
whereas
'Marandu'
'Piatã',
followed
'Xaraés',
exhibited
blades.
pattern
ion
among
attributed
mainly
differences
ability
control
transport
from
shoot,
since
rates
uptake
similarly
salinity.
Tolerance
grasses
genus
is
associated
tissues.
Language: Английский