A Novel Disulfidptosis‐Related Diagnostic Gene Signature and Differential Expression Validation in Ischaemic Cardiomyopathy

Journal of Cellular and Molecular Medicine, Journal Year: 2025, Volume and Issue: 29(5)

Published: March 1, 2025

ABSTRACT Ischaemic cardiomyopathy (IC) predominantly arises from prolonged deprivation of oxygen in the coronary arteries, resulting compromised cardiac contractility or relaxation. This study investigates role disulfidptosis‐associated genes (DiGs) IC. Through analysis datasets GSE5406 and GSE57338, we explored association between DiGs immune characteristics to identify crucial contributing IC development. The support vector machine model emerged as most effective, identifying key such MYH9, NUBPL, MYL6, MYH10 NCKAP1. Validation with independent GSE57345, GSE48166 single‐cell GSE145154 further supported these findings, demonstrating high predictive accuracy. Experimental validation an mouse model, using Western blot, immunohistochemistry RT‐qPCR, confirmed altered expression core myocardial ischaemic regions. research not only elucidates significance but also underscores diagnostic potential identified genes.

Language: Английский

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A Predictive Model Based on the FBXO Family Reveals the Significance of Cyclin F in Hepatocellular Carcinoma

Frontiers in Bioscience-Landmark, Journal Year: 2024, Volume and Issue: 29(5)

Published: May 24, 2024

Objective: The F-box protein (FBXO) family plays a key role in the malignant progression of tumors. However, biological functions and clinical value FBXO liver cancer remain unclear. Our study comprehensively assessed hepatocellular carcinoma (HCC) constructed novel signature based on to predict prognosis guide precision immunotherapy. Methods: Cancer Genome Atlas (TCGA) International Consortium (ICGC) databases were utilized investigate expression characteristics prognostic HCC. A predictive model using TCGA database; its ability was validated ICGC database. Further analyses revealed that this can independently overall survival (OS) rate patients with We further analyzed association signaling pathways, pathological features, somatic mutations, immune therapy responses. Finally, we cyclin F (CCNF) through vitro experiments. Results: involving three genes (CCNF, FBXO43, FBXO45) constructed, effectively identifying high low-risk differences OS, clinicopathological characteristics, cell infiltration status. Additionally, knock-down CCNF HCC lines reduced proliferation vitro, suggesting may be potential therapeutic target for Conclusions: OS response is

Language: Английский

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A novel disulfidptosis-related biomarker for assessing the therapeutic efficacy of a machine learning-based observational study in colon adenocarcinoma

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 11, 2024

Background This study investigated colon adenocarcinoma (COAD), one of the most common types cancer globally. In recent years, a novel cell death pathway, hydrogen sulfide poisoning, has been identified, and targeting disulfide reductase may emerge as new strategy for treatment. However, predictive potential disulfidptosis-related gene (DRGs) in COAD its characteristics tumor immune microenvironment (TIME) remain to be further elucidated. Methods obtained DRGs transcriptome mutation data colorectal samples from Tissue Cancer Genome Atlas (TCGA) database. Pearson differential expression correlation analysis were used identify COAD-related DRGs, risk prognosis model was constructed using univariate least absolute shrinkage selection operator (LASSO) Cox regression analysis. Enrichment then conducted explore biological functions signal transduction differentially expressed genes associated with model. The reliability validated through various statistical analyses such survival analysis, receiver operating characteristic (ROC) curves, calibration plots, bar graphs. relationship between prognostic model, microenvironment, drug sensitivity examined. Finally, specimens patients extracted human protein atlas (HPA) database Yantaishan hospital, compared normal tissues verify level DRGs. Results We have successfully established containing 6 (RPA2, TIMP1, WDR1, POLR3K, KTI12, RTKN). performs well predicting overall COAD. Validation this clinical indicators shows considerable Furthermore, there is significant (TME), infiltrating cells, (p < 0.05). HPA experimental results verified that levels RPA2, KTI12 RTKN tumors higher than those tissues, while WDR1 opposite 0.01). Conclusion identified molecular therapeutic targets response are related targeted therapy provide research direction diagnosis treatment

Language: Английский

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Exploring the diagnostic and immune infiltration roles of disulfidptosis related genes in pulmonary hypertension

Respiratory Research, Journal Year: 2024, Volume and Issue: 25(1)

Published: Oct. 9, 2024

Pulmonary hypertension (PH) is marked by elevated pulmonary artery pressures due to various causes, impacting right heart function and survival. Disulfidptosis, a newly recognized cell death mechanism, may play role in PH, but its associated genes (DiGs) are not well understood this context. This study aims define the diagnostic relevance of DiGs PH.

Language: Английский

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