The role of PAX1 and JAM3 methylation in predicting the pathological upgrading of cervical intraepithelial neoplasia before conization
Abstract
Objective： To
explore
the
effect
of
PAX1
and
JAM3
gene
methylation
on
pathological
upgrading
before
conization.
Methods： A
total
549
patients
who
underwent
colposcopy
at
our
hospital
were
enrolled
for
analysis
from
December
2020
to
April
2022.
results
in
preoperative
cervical
exfoliated
cells
collected.
Univariate
multivariate
logistic
regression
conducted
identify
independent
risk
factors
influencing
conization,
aiming
establish
a
prediction
model.
Results:
A
88
finally
included
statistical
according
inclusion
exclusion
criteria.
Based
univariate
analysis,
∆Ct
(P=0.016,
OR:
0.784,
95%CI:
0.644-0.956)
canal
lesions
(P=0.048,
3.469,
1.014-11.870)
identified
as
Using
above
results,
we
established
model
plotted
receiver
operator
characteristic
curve
(ROC).
The
area
under
(AUC)
was
calculated
when
Youden
index
maximized
with
an
AUC
value
0.818
(95%CI:
0.720-0.916),
specificity
94.4%,
sensitivity
60%.
cut-off
determined
4
.34
maximizing
index.
Conclusion： PAX1
could
be
promising
triage
marker
predicting
CIN
We
found
that
if
△Ct
is
lower
than
4.34,
it
highly
suggestive
upgrading.
Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown
Published: April 17, 2025
Language: Английский