Identification of housekeeping gene for future studies exploring effects of cryopreservation on gene expression in shrimp DOI Creative Commons

Yen-Po Chen,

Chenming Hu,

Sujune Tsai

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 1, 2025

Abstract Few studies have investigated the subcellular effects of low temperature on gene expression in shrimp and most other crustaceans. Before analysis is conducted, suitable housekeeping genes (HKGs) must be confirmed to account for differences reverse transcription process efficiency among samples. Thus, this study aimed verify five frequently used HKGs, namely 18S ribosomal RNA (18S rRNA), ATPase, histone 3, β-actin, glyceraldehyde 3-phosphate dehydrogenase (gapdh) use experiments assessing molecular-scale cryopreservation coral banded ( Stenopus hispidus ) embryos. To conduct chilling studies, we subjected S. embryos incubation at either 26 °C (control) or 5 0, 4, 8, 16, 32 h. The software tools GeNorm, NormFinder, Bestkeeper were employed identify HKG. GeNorm identified 3 rRNA as stable genes. By contrast, NormFinder determined that a eye-formation pre-hatch stage Finally, gapdh β-actin are This first HKGs studying temperatures. Its findings can aid future research evaluating

Language: Английский

Identification of housekeeping gene for future studies exploring effects of cryopreservation on gene expression in shrimp DOI Creative Commons

Yen-Po Chen,

Chenming Hu,

Sujune Tsai

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 1, 2025

Abstract Few studies have investigated the subcellular effects of low temperature on gene expression in shrimp and most other crustaceans. Before analysis is conducted, suitable housekeeping genes (HKGs) must be confirmed to account for differences reverse transcription process efficiency among samples. Thus, this study aimed verify five frequently used HKGs, namely 18S ribosomal RNA (18S rRNA), ATPase, histone 3, β-actin, glyceraldehyde 3-phosphate dehydrogenase (gapdh) use experiments assessing molecular-scale cryopreservation coral banded ( Stenopus hispidus ) embryos. To conduct chilling studies, we subjected S. embryos incubation at either 26 °C (control) or 5 0, 4, 8, 16, 32 h. The software tools GeNorm, NormFinder, Bestkeeper were employed identify HKG. GeNorm identified 3 rRNA as stable genes. By contrast, NormFinder determined that a eye-formation pre-hatch stage Finally, gapdh β-actin are This first HKGs studying temperatures. Its findings can aid future research evaluating

Language: Английский

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