Rehmanniae Radix Preparata ameliorates behavioral deficits and hippocampal neurodevelopmental abnormalities in ADHD rat model DOI Creative Commons
Ruxin Sun,

Haixia Yuan,

Jing Wang

et al.

Frontiers in Neuroscience, Journal Year: 2024, Volume and Issue: 18

Published: May 30, 2024

Objectives Abnormal hippocampal neurodevelopment, particularly in the dentate gyrus region, may be a key mechanism of attention-deficit/hyperactivity disorder (ADHD). In this study, we investigate effect most commonly used Chinese herb for treatment ADHD, Rehmanniae Radix Preparata (RRP), on behavior and neurodevelopment spontaneously hypertensive rats (SHR). Methods Behavior tests, including Morris water maze (MWM) test, open field test (OFT) elevated plus (EPM) were performed to assess RRP hyperactive impulsive behavior. Hippocampal was characterized by transmission electron microscopy, immunofluorescence, Golgi staining Nissl approaches. Regulatory proteins such as Trkb, CDK5, FGF2/FGFR1 examined Western blot analysis. Results The results showed that could effectively control spontaneous improve spatial learning memory ability. significantly reduced neuronal loss increased number stem cells, promoted synaptic plasticity. addition, FGF/FGFR signaling upregulated after treatment. Conclusion can reduce ameliorate neurodevelopmental abnormalities ADHD rat model.

Language: Английский

Parental origin of transgene modulates amyloid-β plaque burden in the 5xFAD mouse model of Alzheimer’s disease DOI Creative Commons
Andrew Octavian Sasmita,

Erinne Cherisse Ong,

T. I. Nazarenko

et al.

Neuron, Journal Year: 2025, Volume and Issue: 113(6), P. 838 - 846.e4

Published: Jan. 20, 2025

In Alzheimer's disease (AD) research, the 5xFAD mouse model is commonly used as a heterozygote crossed with other genetic models to study AD pathology. We investigated whether parental origin of transgene affects plaque deposition. Using quantitative light-sheet microscopy, we found that paternal inheritance led 2-fold higher burden compared maternal inheritance, finding consistent across multiple colonies. This effect was not due gestation in or rearing by females. Immunoblotting suggested transgenic modulates protein expression, potentially genomic imprinting Thy1.2 promoter. Surprisingly, fewer than 20% studies report breeding schemes, suggesting this factor might confound previous findings. Our data highlight significant determinant mice and underscore importance reporting improve scientific rigor reproducibility research.

Language: Английский

Citations

4
Deciphering the role of APOE in cerebral amyloid angiopathy: from genetic insights to therapeutic horizons DOI Creative Commons

H. B. Hu,

Suk-King Wan,

Yongxian Hu

et al.

Annals of Medicine, Journal Year: 2025, Volume and Issue: 57(1)

Published: Jan. 2, 2025

Cerebral amyloid angiopathy (CAA), characterized by the deposition of amyloid-β (Aβ) peptides in walls medium and small vessels brain leptomeninges, is a major cause lobar hemorrhage elderly individuals. Among genetic risk factors for CAA that continue to be recognized, apolipoprotein E (APOE) gene most significant prevalent, as its variants have been implicated more than half all patients with CAA. While presence APOE ε4 allele markedly increases CAA, ε2 confers protective effect relative common ε3 allele. These allelic encode three isoforms differ at two amino acid positions. The primary physiological role mediate lipid transport periphery; however, it has also shown involved wide array biological functions, particularly those involving Aβ, which plays known processing, production, aggregation, clearance. challenges posed reliance on postmortem histological analyses current absence an effective intervention underscore urgency innovative APOE-targeted strategies diagnosing This review not only deepens our understanding impact pathogenesis but can help guide exploration targeted therapies, inspiring further research into therapeutic potential APOE.

Language: Английский

Citations

0
Neuroprotective potential of ApoE-mimetic peptide (ApoEFrag) in stroke models: Neurobehavioural and mechanistic study DOI
Sakeel Ahmed,

Rajan K. Tripathy,

Abhay H. Pande

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 140790 - 140790

Published: Feb. 1, 2025

Language: Английский

Citations

0
Rehmanniae Radix Preparata ameliorates behavioral deficits and hippocampal neurodevelopmental abnormalities in ADHD rat model DOI Creative Commons
Ruxin Sun,

Haixia Yuan,

Jing Wang

et al.

Frontiers in Neuroscience, Journal Year: 2024, Volume and Issue: 18

Published: May 30, 2024

Objectives Abnormal hippocampal neurodevelopment, particularly in the dentate gyrus region, may be a key mechanism of attention-deficit/hyperactivity disorder (ADHD). In this study, we investigate effect most commonly used Chinese herb for treatment ADHD, Rehmanniae Radix Preparata (RRP), on behavior and neurodevelopment spontaneously hypertensive rats (SHR). Methods Behavior tests, including Morris water maze (MWM) test, open field test (OFT) elevated plus (EPM) were performed to assess RRP hyperactive impulsive behavior. Hippocampal was characterized by transmission electron microscopy, immunofluorescence, Golgi staining Nissl approaches. Regulatory proteins such as Trkb, CDK5, FGF2/FGFR1 examined Western blot analysis. Results The results showed that could effectively control spontaneous improve spatial learning memory ability. significantly reduced neuronal loss increased number stem cells, promoted synaptic plasticity. addition, FGF/FGFR signaling upregulated after treatment. Conclusion can reduce ameliorate neurodevelopmental abnormalities ADHD rat model.

Language: Английский

Citations

0