Low-cost physiology and behavioral monitor for intravital imaging in small mammals

Neurophotonics, Journal Year: 2025, Volume and Issue: 12(01)

Published: Jan. 25, 2025

SignificanceFunctional brain imaging experiments in awake animals require meticulous monitoring of animal behavior to screen for spontaneous behavioral events. Although these events occur naturally, they can alter cell signaling and hemodynamic activity the confound functional measurements.AimWe developed a centralized, user-friendly, stand-alone platform that includes an fixation frame, compact peripheral sensors, portable data acquisition system. The affordable, integrated benefit by motion detection alertness levels as complementary readouts measurements.ApproachA custom system was designed using powerful, inexpensive microcomputer. We customized accelerometer miniature camera modules efficient, real-time pupil diameter. then tested validated platform's performance with optical intrinsic signal GCaMP fluorescence calcium activation mice.ResultsThe shows promise detecting dilation while imaging. Stimulus-induced found initiate earlier than cortical hemodynamics slower rise time. Compared neuronal response, stimulus-induced initiated later time.ConclusionsWe monitor dynamics. device be easily coupled synchronized systems behavior, alertness, studies.

Language: Английский

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Peripheral inflammation is accompanied by cerebral hypoperfusion in mice

Frontiers in Pain Research, Journal Year: 2025, Volume and Issue: 6

Published: April 8, 2025

Chronic pain is a disabling condition that accompanied by neuropsychiatric comorbidities such as anxiety, depression, and cognitive decline. While the peripheral alterations are well-studied, we lack an understanding of how these changes can result in long-lasting brain ensuing behavioral phenotypes. This study aims to quantify cerebral blood perfusion using laser speckle contrast imaging (LSCI) murine Complete Freund's adjuvant (CFA) model unilateral inflammation. Twenty four adult male female C57BL/6 mice were randomly assigned control (0.05 ml saline) or 1 3 experimental groups receiving CFA (0.01 ml, 0.05 0.1 ml) on right hindpaw. Three days after intraplantar injections, animals examined for signs inflammation subjected craniotomy vivo LSCI parietal-temporal lobes. Unilateral administration resulted local well hypoperfusion dose-dependent manner. To our knowledge, this first examine effects CFA-induced perfusion. It serves step delineating path which insult tissues cause plasticity via vascular mechanisms.

Language: Английский

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Spinal cord perfusion impairments in the M83 mouse model of Parkinson’s disease

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 29, 2024

Abstract Metabolism and bioenergetics in the central nervous system play important roles pathophysiology of Parkinson’s disease (PD). Here, we employed a multimodal imaging approach to assess oxygenation changes spinal cord transgenic M83 murine model PD comparison non-transgenic littermates at 9-12 months-of-age. A lower oxygen saturation (SO 2 ) SVOT was detected vivo with spiral volumetric optoacoustic tomography (SVOT) mice compared littermate mice. Ex-vivo high-field T1-weighted magnetic resonance (MRI) immunostaining for alpha-synuclein (phospho-S129) vascular organisation (CD31 GLUT1) were used investigate nature abnormalities via imaging. analysis showed that network not impaired MRI assisted deep learning-based automatic segmentation no atrophy littermates, whereas nuclear phosphorylated Ser129 site could be linked early pathology metabolic dysfunction. The proposed validated non-invasive high-resolution tool study holds promise assessing preceding motor deficits

Language: Английский

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Spiral volumetric optoacoustic tomography of reduced oxygen saturation in the spinal cord of M83 mouse model of Parkinson’s disease

European Journal of Nuclear Medicine and Molecular Imaging, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 9, 2024

Abstract Purpose Metabolism and bioenergetics in the central nervous system play important roles pathophysiology of Parkinson’s disease (PD). Here, we employed a multimodal imaging approach to assess oxygenation changes spinal cord transgenic M83 murine model PD overexpressing mutated A53T alpha-synuclein form comparison with non-transgenic littermates. Methods In vivo spiral volumetric optoacoustic tomography (SVOT) was performed oxygen saturation (sO 2 ) cords mice Ex high-field T1-weighted (T1w) magnetic resonance (MRI) at 9.4T used alterations cord. 3D SVOT analysis deep learning-based automatic segmentation T1w MRI data for mouse were developed quantification. Immunostaining phosphorylated (pS129 α-syn), as well vascular organization (CD31 GLUT1), after scan. Results revealed lower sO compared littermates sub-100 μm spatial resolution. MRI-assisted by in-house (validated manual analysis) no atrophy 50 The network not impaired presence pS129 α-syn accumulation. Conclusion We tools deep-learning-based structural data, data. demonstrated non-invasive high-resolution reduced absence model.

Language: Английский

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Peripheral Inflammation is accompanied by Cerebral Hypoperfusion in Mice

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 5, 2024

Chronic pain is a disabling condition that accompanied by neuropsychiatric comorbidities such as anxiety, depression, and cognitive decline. While the peripheral alterations are well-studied, we lack an understanding of how these changes can result in long-lasting brain ensuing behavioral phenotypes. This study aims to quantify cerebral blood perfusion using laser speckle contrast imaging (LSCI) murine Complete Freund's adjuvant (CFA) model unilateral inflammation.

Language: Английский

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