Biomedicines,
Journal Year:
2023,
Volume and Issue:
11(3), P. 843 - 843
Published: March 10, 2023
The
periodontal
ligament
is
located
between
the
bone
(alveolar
bone)
and
cementum
of
tooth,
it
connected
by
tough
fibers
called
Sharpey's
fibers.
To
maintain
healthy
teeth,
foundation
supporting
teeth
must
be
healthy.
Periodontal
diseases,
also
known
as
tooth
loss,
cause
alveolar
to
dissolve.
bone,
similar
bones
in
other
body
parts,
repeatedly
resorbed
osteoclasts
renewed
osteogenic
cells.
This
means
that
an
old
constantly
being
replaced
a
new
bone.
In
around
absorbed,
disease
progresses,
shrinks
gradually.
most
cases,
does
not
return
its
original
form
even
after
cured.
Gum
covers
surface
so
matches
shape
exposing
more
than
before,
making
look
longer,
leaving
gaps
some
cases
causing
sting.
Previously,
only
treatment
for
diseases
was
stop
from
progressing
further
before
fell
out,
restoration
condition
almost
impossible.
However,
method
can
help
regeneration
tissues
destroyed
their
state
much
possible
introduced.
Recently,
with
improvements
implant
material
properties,
therapy
has
become
indispensable
dentistry
important
prosthetic
option.
Treatment
methods
techniques,
which
are
mainly
based
on
experience,
have
gradually
accumulated
scientific
evidence,
number
indications
increased.
development
augmentation
contributed
remarkably
expansion
indications,
this
been
made
various
advances
materials
science.
induced
pluripotent
stem
cell
(iPS)
technology
regenerating
tissues,
including
expected
applied
such
loss
periodontitis.
review
focuses
describes
clinical
practice,
latest
basic
research.
Stem Cell Research & Therapy,
Journal Year:
2022,
Volume and Issue:
13(1)
Published: Sept. 5, 2022
Abstract
Background
Increasing
studies
have
reported
the
therapeutic
effect
of
mesenchymal
stem
cell
(MSC)-derived
exosomes
by
which
protein
and
miRNA
are
clearly
characterized.
However,
proteomics
profiles
derived
from
human
embryonic
cells
(hESCs)
human-induced
pluripotent
(hiPSCs)
remain
unclear.
Methods
In
this
study,
we
isolated
hESCs,
hiPSCs,
umbilical
cord
(hUC-MSCs)
via
classic
ultracentrifugation
a
0.22-μm
filter,
followed
conservative
identification.
Tandem
mass
tag
labeling
label-free
relative
peptide
quantification
together
defined
their
proteomics.
High-throughput
sequencing
was
performed
to
determine
profiles.
Then,
conducted
bioinformatics
analysis
identify
dominant
biological
processes
pathways
modulated
exosome
cargos.
Finally,
western
blot
RT-qPCR
were
detect
actual
loads
proteins
miRNAs
in
three
types
exosomes.
Results
Based
on
our
cargos
contribute
sophisticated
processes.
comparison,
hESC
(hESC-Exos)
superior
regulating
development,
metabolism,
anti-aging,
hiPSC
(hiPSC-Exos)
had
similar
functions
as
hESC-Exos,
whereas
hUC-MSCs
(hUC-MSC-Exos)
contributed
more
immune
regulation.
Conclusions
The
data
presented
study
help
define
landscapes
exosomes,
predict
systematic
comprehensive
network
at
system
level,
reveal
respective
potential
applications
different
fields
so
optimize
selection
preclinical
clinical
trials.
International Journal of Molecular Sciences,
Journal Year:
2022,
Volume and Issue:
23(7), P. 3869 - 3869
Published: March 31, 2022
Soft
tissue
defects
are
common
following
trauma
and
tumor
extirpation.
These
injuries
can
result
in
poor
functional
recovery
lead
to
a
diminished
quality
of
life.
The
healing
skin
muscle
is
complex
process
that,
at
present,
leads
incomplete
scarring.
Regenerative
medicine
may
offer
the
opportunity
improve
outcomes.
Barriers
regenerative
strategies
have
included
cost,
regulatory
hurdles,
need
for
cell-based
therapies.
In
recent
years,
exosomes,
or
extracellular
vesicles,
gained
tremendous
attention
field
soft
repair
regeneration.
nanosized
particles
(30-140
nm)
break
cellular
boundaries,
as
well
facilitate
intracellular
signal
delivery
various
physiologic
pathologic
processes.
Existing
studies
established
potential
exosomes
regenerating
tendons,
skeletal
muscles,
peripheral
nerves
through
different
mechanisms,
including
promoting
myogenesis,
increasing
tenocyte
differentiation
enhancing
neurite
outgrowth,
proliferation
Schwann
cells.
be
stored
immediate
use
operating
room,
produced
cost
efficiently.
this
article,
we
critically
review
current
advances
(tendons,
nerves)
healing.
Additionally,
new
directions
clinical
applications
future
will
discussed.
Small,
Journal Year:
2023,
Volume and Issue:
19(20)
Published: Feb. 17, 2023
Abstract
Exosomes
are
promising
new
biomarkers
for
colorectal
cancer
(CRC)
diagnosis,
due
to
their
rich
biological
fingerprints
and
high
level
of
stability.
However,
the
accurate
detection
exosomes
with
specific
surface
receptors
is
limited
clinical
application.
Herein,
an
exosome
enrichment
platform
on
a
3D
porous
sponge
microfluidic
chip
constructed
capture
efficiency
this
≈90%.
Also,
deep
mass
spectrometry
analysis
followed
by
multi‐level
expression
screenings
revealed
CRC‐specific
membrane
protein
(SORL1).
A
method
SORL1
quantum
dot
labeling
further
designed
ensemble
classification
system
established
extracting
features
from
64‐patched
fluorescence
images.
Importantly,
area
under
curve
(AUC)
using
0.99,
which
significantly
higher
(
p
<
0.001)
than
that
conventional
biomarker
(carcinoembryonic
antigen
(CEA),
AUC
0.71).
The
above
showed
similar
diagnostic
performance,
dealing
early‐stage
CRC,
young
CEA‐negative
CRC
patients.
Biomedicines,
Journal Year:
2023,
Volume and Issue:
11(3), P. 843 - 843
Published: March 10, 2023
The
periodontal
ligament
is
located
between
the
bone
(alveolar
bone)
and
cementum
of
tooth,
it
connected
by
tough
fibers
called
Sharpey's
fibers.
To
maintain
healthy
teeth,
foundation
supporting
teeth
must
be
healthy.
Periodontal
diseases,
also
known
as
tooth
loss,
cause
alveolar
to
dissolve.
bone,
similar
bones
in
other
body
parts,
repeatedly
resorbed
osteoclasts
renewed
osteogenic
cells.
This
means
that
an
old
constantly
being
replaced
a
new
bone.
In
around
absorbed,
disease
progresses,
shrinks
gradually.
most
cases,
does
not
return
its
original
form
even
after
cured.
Gum
covers
surface
so
matches
shape
exposing
more
than
before,
making
look
longer,
leaving
gaps
some
cases
causing
sting.
Previously,
only
treatment
for
diseases
was
stop
from
progressing
further
before
fell
out,
restoration
condition
almost
impossible.
However,
method
can
help
regeneration
tissues
destroyed
their
state
much
possible
introduced.
Recently,
with
improvements
implant
material
properties,
therapy
has
become
indispensable
dentistry
important
prosthetic
option.
Treatment
methods
techniques,
which
are
mainly
based
on
experience,
have
gradually
accumulated
scientific
evidence,
number
indications
increased.
development
augmentation
contributed
remarkably
expansion
indications,
this
been
made
various
advances
materials
science.
induced
pluripotent
stem
cell
(iPS)
technology
regenerating
tissues,
including
expected
applied
such
loss
periodontitis.
review
focuses
describes
clinical
practice,
latest
basic
research.
