Research Square (Research Square),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Dec. 15, 2023
The
biological
aging
of
mesenchymal
stem
cells
is
proposed
to
contribute
the
development
a
range
musculoskeletal
and
systemic
diseases
associated
with
older
adults,
such
as
osteoporosis,
sarcopenia,
frailty.
Despite
this,
little
understood
about
specific
mechanisms
which
drive
this
cell
exhaustion,
most
studies
evaluating
indirect
effects
other
changes,
DNA
damage,
senescence,
inflammaging.
In
study,
we
assess
transcriptomic
proteomic
changes
in
three
different
populations
progenitor
from
(50-70
years)
younger
(20-40
individuals
uncover
potential
driving
exhaustion
tissues.
To
do
harvested
primary
bone
marrow
(MPCs),
circulating
osteoprogenitors
(COP),
adipose-derived
(ADSCs)
donors,
an
equal
number
samples
males
females.
These
underwent
RNA
sequencing
label-free
analysis,
comparing
ones.
There
was
distinct
phenotype
pooled
cells,
indicative
suppressed
proliferation
differentiation;
however,
there
no
consistent
change
proteome
cells.
Older
MPCs
had
both
transcriptome
proteome,
again
altered
differentiation
proliferation,
but
also
pro-inflammatory
immune
shift
adults.
COP
showed
strong
signaling
phenotype.
Similarly,
ADSCs
displayed
physiologies
migration,
adherence,
activation,
age.
results
show
that
are
underlying
likely
decline
tissue
regeneration;
contextual
factors
microenvironment
general
health
status
have
role
this.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(23), P. 13145 - 13145
Published: Dec. 6, 2024
Regenerative
therapy
involving
stem
cell
transplantation
has
become
an
option
for
the
radical
treatment
of
diabetes
mellitus.
Disruption
in
clock
genes
cells
affects
homeostasis
transplanted
tissues.
We
examined
circadian
rhythm
adipose-derived
mesenchymal
derived
from
a
patient
with
type
2
mellitus
(T2DM-ADSC).
The
(PER2,
CLOCK1,
CRY1,
and
ARNTL[BMAL1])
exhibited
similar
daily
fluctuations
phase
amplitude
between
group
healthy
individual
(N-ADSC)
T2DM-ADSC.
findings
demonstrated
that
are
synchronized
those
living
organisms.
Next-generation
sequencing
was
then
employed
to
categorize
variation
expression
N-ADSC
MTATP8P1
NDUFA7_2
gene
significantly
reduced
at
two
time
points
(ZT6
ZT18),
were
lost.
present
study
reports,
first
time,
rhythms
NDUFA7_2,
involved
mitochondrial
processes,
altered
Nutrients,
Journal Year:
2024,
Volume and Issue:
16(7), P. 1004 - 1004
Published: March 29, 2024
Extensive
in
vivo
investigations
have
demonstrated
the
antioxidant
properties
of
fish
collagen
oligopeptides
(FCOPs).
One
main
causes
aging
and
chronic
non-communicable
diseases
is
oxidative
stress.
Therefore,
FCOPs
a
broad
range
applications
illness
prevention
delaying
from
standpoint
"food
medicine"
theory.
However,
mechanisms
that
underpin
activity
are
not
completely
understood.
The
specific
objective
this
essay
was
to
investigate
effect
its
possible
mechanism
at
cellular
level.
Mouse
embryonic
fibroblasts
NIH/3T3
human
vein
endothelial
cells
(HUVECs)
were
exposed
200
µM
hydrogen
peroxide
containing
different
concentrations
for
4
h
supplemented
with
24
h.
Normal
growth
medium
without
applied
control
cells.
An
array
assays
used
evaluate
implications
on
stress
status,
homeostasis,
inflammatory
levels,
mitochondrial
function.
We
found
exerted
protective
by
inhibiting
reactive
oxygen
species
(ROS)
production,
enhancing
superoxide
dismutase
(SOD)
nitric
oxide
synthase
(eNOS)
activities
cell
viability,
cycle
arrest
G1
phase,
suppressing
interleukin-1β
(IL-1β),
IL-6,
matrix
metalloproteinase-3
(MMP-3)
intercellular
adhesion
molecule-1(ICAM-1)
secretion,
downregulating
nuclear
factor-kappa
B
(NF-κB)
activity,
protecting
membrane
potential,
increasing
ATP
synthesis
NAD+
both
had
stronger
impact
than
HUVECs,
simultaneously
glutathione
peroxidase
(GSH-Px)
decreasing
malondialdehyde
(MDA)
content
NIH/3T3.
These
findings
indicate
effects
tissue
damaged
therefore
promote
inhibit
inflammation,
protect
mitochondria.
Meanwhile,
better
health
outcomes
will
be
achieved
thoroughly
investigating
effective
dose
intervention
time
FCOPs,
as
absorption
efficiency
varies
The Journals of Gerontology Series A,
Journal Year:
2024,
Volume and Issue:
79(9)
Published: June 5, 2024
The
biological
aging
of
stem
cells
(exhaustion)
is
proposed
to
contribute
the
development
a
variety
age-related
conditions.
Despite
this,
little
understood
about
specific
mechanisms
which
drive
this
process.
In
study,
we
assess
transcriptomic
and
proteomic
changes
in
3
different
populations
mesenchymal
progenitor
from
older
(50-70
years)
younger
(20-40
individuals
uncover
potential
driving
cell
exhaustion
tissues.
To
do
harvested
primary
bone
marrow
(MPCs),
circulating
osteoprogenitors
(COP),
adipose-derived
(ADSCs)
donors,
with
an
equal
number
samples
men
women.
These
underwent
RNA
sequencing
label-free
analysis,
comparing
ones.
There
was
distinct
phenotype
analysis
pooled
cells,
suggestive
suppressed
proliferation
differentiation;
however,
these
were
not
reflected
proteome
cells.
Analyzed
independently,
MPCs
had
both
transcriptome
consistent
altered
differentiation
proinflammatory
immune
shift
adults.
COP
showed
signaling
but
no
phenotype.
Similarly,
ADSCs
displayed
shifts
physiologies
associated
migration,
adherence,
activation
change
age.
results
show
that
there
are
underlying
may
decline
tissue
regeneration.
However,
inconsistently
regulated.
Drug Design Development and Therapy,
Journal Year:
2024,
Volume and Issue:
Volume 18, P. 3903 - 3919
Published: Aug. 1, 2024
Bone
loss
is
a
common
complication
of
type
2
diabetes
mellitus
(T2DM).
Circadian
rhythms
play
significant
role
in
T2DM
and
bone
remodeling.
Eldecalcitol
(ED-71),
novel
active
vitamin
D
analog,
has
shown
promise
ameliorating
T2DM.
We
aimed
to
investigate
whether
the
circadian
rhythm
coregulator
BMAL1
mediates
anti-osteoporotic
effect
ED-71
its
associated
mechanisms.
Research Square (Research Square),
Journal Year:
2023,
Volume and Issue:
unknown
Published: July 21, 2023
Abstract
Regenerative
therapy
involving
stem
cell
transplantation
has
become
an
option
for
the
radical
treatment
of
diabetes
mellitus.
Disruption
in
clock
genes
cells
affects
homeostasis
transplanted
tissues.
This
is
possibly
first
study
to
examine
circadian
rhythm
adipose-derived
mesenchymal
derived
from
a
patient
with
type
2
mellitus
(T2DM-ADSC).
The
(
PER2
,
CLOCK1
CRY1
and
ARNTL
[
BMAL1
])
exhibited
similar
daily
fluctuations
phase
amplitude
between
group
healthy
individual
(N-ADSC)
T2DM-ADSC.
findings
demonstrated
that
are
synchronized
those
living
organisms.
Moreover,
mitochondrial
functions
showed
N-ADSC
group.
However,
such
were
not
noted
T2DM-ADSC
In
group,
MTATP8P1
NDUFA7_2
disappeared.
transplant,
results
number
DNA
copies,
Mitophagy,
membrane
potential
NF-kB
signaling.
contrast,
no
observed
transplant.
rhythms
function
signaling
revealed
this
may
be
new
marker
efficiency
patients
diabetes.
Research Square (Research Square),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Dec. 15, 2023
The
biological
aging
of
mesenchymal
stem
cells
is
proposed
to
contribute
the
development
a
range
musculoskeletal
and
systemic
diseases
associated
with
older
adults,
such
as
osteoporosis,
sarcopenia,
frailty.
Despite
this,
little
understood
about
specific
mechanisms
which
drive
this
cell
exhaustion,
most
studies
evaluating
indirect
effects
other
changes,
DNA
damage,
senescence,
inflammaging.
In
study,
we
assess
transcriptomic
proteomic
changes
in
three
different
populations
progenitor
from
(50-70
years)
younger
(20-40
individuals
uncover
potential
driving
exhaustion
tissues.
To
do
harvested
primary
bone
marrow
(MPCs),
circulating
osteoprogenitors
(COP),
adipose-derived
(ADSCs)
donors,
an
equal
number
samples
males
females.
These
underwent
RNA
sequencing
label-free
analysis,
comparing
ones.
There
was
distinct
phenotype
pooled
cells,
indicative
suppressed
proliferation
differentiation;
however,
there
no
consistent
change
proteome
cells.
Older
MPCs
had
both
transcriptome
proteome,
again
altered
differentiation
proliferation,
but
also
pro-inflammatory
immune
shift
adults.
COP
showed
strong
signaling
phenotype.
Similarly,
ADSCs
displayed
physiologies
migration,
adherence,
activation,
age.
results
show
that
are
underlying
likely
decline
tissue
regeneration;
contextual
factors
microenvironment
general
health
status
have
role
this.
