Journal of Cellular and Molecular Medicine,
Journal Year:
2024,
Volume and Issue:
28(23)
Published: Dec. 1, 2024
The
differentiation
of
mesenchymal
stem
cells
(MSCs)
into
hepatocyte-like
(HLCs)
is
considered
one
the
most
promising
strategies
for
alternative
hepatocyte
transplantation
to
treat
end-stage
liver
disease.
To
advance
this
method,
it
crucial
gain
a
deeper
understanding
mechanisms
governing
hepatogenic
differentiation.
study
demonstrated
that
suppression
intracellular
domain
release
Notch
pathway
receptor
via
γ-secretase
inhibitor
N-[(3,
5-difluorophenyl)acetyl]-L-alanyl-2-phenylglycine-1,
1-dimethylethyl
ester
(DAPT)
significantly
promotes
expression
hepatocyte-related
genes
and
proteins
in
HLCs.
Increased
inducible
NO
synthase
(iNOS)
during
led
elevated
endogenous
production.
Biotin
switch
assays
revealed
gradual
increase
S-nitrosylation
(SNO)-NOTCH1
decrease
overall
NOTCH1
addition
exogenous
donor
S-nitrosoglutathione
(GSNO)
SNO
dithiothreitol
(DTT)
further
SNO-NOTCH1
MSCs
mature
hepatocytes.
Briefly,
our
results
fully
modification
extracellular
by
NO,
leading
formation
SNO-NOTCH1,
inhibiting
signalling
pathway.
Our
highlights
critical
role
regulating
offers
new
insights
driving
process.
Stem Cell Research & Therapy,
Journal Year:
2023,
Volume and Issue:
14(1)
Published: Sept. 4, 2023
Abstract
Although
mesenchymal
stem
cell
(MSC)
transplantation
provides
an
alternative
strategy
for
end-stage
liver
disease
(ESLD),
further
widespread
application
of
MSC
therapy
is
limited
owing
to
low
engraftment
efficiency.
Improving
efficiency
plays
a
critical
role
in
enhancing
diseases.
In
this
review,
we
summarize
the
current
status
and
challenges
ESLD.
We
also
outline
complicated
cell-homing
process
highlight
how
closely
related
huge
differences
extracellular
conditions
involved
homing
journeys
ranging
from
constant,
controlled
vitro
variable
challenging
vivo.
survival
capabilities
enhances
efficacy.
Therefore,
strategies,
including
hypoxic
priming,
drug
pretreatment,
gene
modification,
cytokine
as
well
splenectomy
local
irradiation,
used
improve
capability,
enhance
therapeutic
therapy.
hope
that
review
will
provide
new
insights
into
The Egyptian Journal of Internal Medicine,
Journal Year:
2024,
Volume and Issue:
36(1)
Published: Feb. 12, 2024
Abstract
Background
Liver
fibrosis
results
from
chronic
liver
injury
and
is
characterized
by
excessive
deposition
of
extracellular
matrix
proteins
including
collagen.
It
can
progress
to
cirrhosis
failure.
Main
body
the
abstract
Multiple
cellular
signaling
pathways
drive
hepatic
stellate
cell
activation
fibrogenesis.
Advances
in
biomarkers,
imaging
modalities,
omics
platforms
enable
noninvasive
diagnosis
staging
fibrosis.
Emerging
antifibrotic
approaches
include
medications
like
pirfenidone,
obeticholic
acid,
monoclonal
antibodies
targeting
pro-fibrotic
mediators.
Cell
therapies
using
mesenchymal
stem
cells
demonstrate
potential
through
paracrine
immunosuppression.
Tissue-engineered
grafts
biomaterial
carriers
for
localized
drug
delivery
are
promising
technologies.
Microfluidic
liver-on-a-chip
with
patient-derived
provide
unprecedented
models
study
human
test
candidates.
Short
conclusion
Significant
has
elucidated
mechanisms
underlying
fibrogenesis
uncovered
novel
therapeutic
targets.
Ongoing
challenges
translating
preclinical
findings,
improving
efficacy,
enabling
personalized
precision
medicine
approaches.
Further
research
into
combinatorial
therapies,
tissue
engineering
technologies
will
advance
treatment
all
causes.
Experimental Gerontology,
Journal Year:
2023,
Volume and Issue:
184, P. 112334 - 112334
Published: Nov. 25, 2023
Recent
scientific
interest
has
been
directed
towards
age-related
diseases,
driven
by
the
significant
increase
in
global
life
expectancy
and
growing
population
of
individuals
aged
65
above.
The
ageing
process
encompasses
various
biological,
physiological,
environmental,
psychological,
behavioural,
social
changes,
leading
to
an
augmented
susceptibility
chronic
illnesses.
Cardiovascular,
neurological,
musculoskeletal,
liver
oncological
diseases
are
prevalent
elderly.
Moreover,
demonstrate
reduced
regenerative
capacity
decreased
tolerance
therapeutic
interventions,
including
organ
transplantation.
Liver
such
as
non-alcoholic
fatty
disease,
alcoholic
hepatitis,
fibrosis,
cirrhosis,
have
emerged
public
health
concerns.
Paradoxically,
these
conditions
remain
underestimated
despite
their
substantial
impact.
Age-related
factors
closely
associated
with
severity
unfavorable
prognosis
warranting
further
investigation
enhance
clinical
management
develop
novel
strategies.
This
comprehensive
review
focuses
specifically
on
treatment
strategies,
contemporary
practices.
It
provides
a
detailed
account
burden,
types,
molecular
mechanisms,
epigenetic
alterations
underlying
pathologies.
eGastroenterology,
Journal Year:
2024,
Volume and Issue:
2(1), P. e100040 - e100040
Published: March 1, 2024
Liver
fibrosis
is
the
formation
of
a
fibrous
scar
due
to
chronic
liver
disease
including
viral
hepatitis,
alcohol
and
non-alcoholic
fatty
disease.
Without
treatment,
it
will
develop
into
cirrhosis
hepatocellular
carcinoma.
Up
now,
there
no
effective
way
cure
fibrosis.
Extracellular
vesicles
(EVs)
are
biological
nanoparticles
with
potential
be
therapeutical
agents
or
delivery
tools.
A
lot
studies
have
demonstrated
effect
EVs
on
In
this
review,
we
mainly
pay
attention
roles
non-parenchymal
cells
in
pathology
fibrosis,
basic
information
about
when
they
act
cells.
Frontiers in Bioengineering and Biotechnology,
Journal Year:
2023,
Volume and Issue:
11
Published: Dec. 13, 2023
Liver
disease
has
emerged
as
a
significant
worldwide
health
challenge
due
to
its
diverse
causative
factors
and
therapeutic
complexities.
The
majority
of
liver
diseases
ultimately
progress
end-stage
transplantation
remains
the
only
effective
therapy
with
limitations
donor
organ
shortage,
lifelong
immunosuppressants
expensive
treatment
costs.
Numerous
pre-clinical
studies
have
revealed
that
extracellular
vesicles
released
by
mesenchymal
stem
cells
(MSC-EV)
exhibited
considerable
potential
in
treating
diseases.
Although
natural
MSC-EV
many
advantages,
some
characteristics
MSC-EV,
such
heterogeneity,
uneven
effect,
rapid
clearance
vivo
constrain
clinical
translation.
In
recent
years,
researchers
explored
plenty
ways
improve
efficacy
rotation
rate
disease.
this
review,
we
summarized
current
strategies
enhance
potency
mainly
including
optimization
culture
conditions
MSC
or
modifications
aiming
facilitate
development
application
Frontiers in Immunology,
Journal Year:
2023,
Volume and Issue:
14
Published: Sept. 7, 2023
Acute
liver
failure
(ALF)
is
a
high-mortality
syndrome
for
which
transplantation
considered
the
only
effective
treatment
option.
A
shortage
of
donor
organs,
high
costs
and
surgical
complications
associated
with
immune
rejection
constrain
therapeutic
effects
transplantation.
Recently,
mesenchymal
stem
cell
(MSC)
therapy
was
recognized
as
an
alternative
strategy
Bone
marrow
cells
(BMSCs)
have
been
used
in
clinical
trials
several
diseases
due
to
their
ease
acquisition,
strong
proliferation
ability,
multipotent
differentiation,
homing
lesion
site,
low
immunogenicity
anti-inflammatory
antifibrotic
effects.
In
this
review,
we
comprehensively
summarized
harvest
culture
expansion
strategies
BMSCs,
development
animal
models
ALF
different
aetiologies,
critical
mechanisms
BMSC
challenge
application.
Stem Cells and Development,
Journal Year:
2024,
Volume and Issue:
33(9-10), P. 214 - 227
Published: March 6, 2024
Cellular
therapies
provide
promising
options
for
inducing
tolerance
in
transplantation
of
solid
organs,
bone
marrow,
and
vascularized
composite
allografts.
However,
novel
tolerance-inducing
protocols
remain
limited,
despite
extensive
research.
We
previously
introduced
characterized
a
human
multi-chimeric
cell
(HMCC)
line,
created
through
ex
vivo
fusion
umbilical
cord
blood
(UCB)
cells
derived
from
three
unrelated
donors.
In
this
study,
we
assessed
biodistribution
safety
HMCCs
the
NOD.Cg-Prkdc
