Asian Journal of Surgery, Journal Year: 2024, Volume and Issue: unknown
Published: Nov. 1, 2024
Language: Английский
Advanced Science, Journal Year: 2024, Volume and Issue: 11(31)
Published: June 19, 2024
Abstract Spinal cord injury (SCI) is a severe neurological condition that frequently leads to significant sensory, motor, and autonomic dysfunction. This study sought delineate the potential mechanistic underpinnings of extracellular vesicles (EVs) derived from ginsenoside Rg1‐pretreated neuronal cells (Rg1‐EVs) in ameliorating SCI. These results demonstrated treatment with Rg1‐EVs substantially improved motor function spinal cord‐injured mice. enhance microglial polarization toward M2 phenotype repressed oxidative stress, thereby altering immune responses decreasing inflammatory cytokine secretion. Moreover, diminish reactive oxygen species accumulation enhanced neural tissue repair by regulating mitochondrial function. Proteomic profiling highlighted enrichment MYCBP2 Rg1‐EVs, functional assays confirmed knockdown counteracted beneficial effects vitro vivo. Mechanistically, implicated ubiquitination degradation S100A9, promoting M2‐phenotype reducing stress. Overall, these findings substantiated pivotal role protection recovery following SCI through MYCBP2‐mediated S100A9. research offers novel insights into therapeutic strategies against supports clinical Rg1‐EVs.
Language: Английский
Citations
5
Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)
Published: July 31, 2024
Spinal cord injury (SCI) compromises the blood-spinal barrier (BSCB) and induces neuroinflammation, potentially exacerbating neuronal damage. This underscores importance of maintaining BSCB integrity mitigating neuroinflammation in SCI treatment. Our study explores an innovative approach to treating by utilizing platelet-rich plasma-derived exosomes (PRP-Exos) stabilize function alleviate neuroinflammation. We successfully isolated from plasma conducted both vivo vitro experiments assess therapeutic effects PRP-Exos explore their potential mechanisms stabilizing BSCB, reducing promoting neural functional recovery.In results demonstrate that significantly reduce permeability bEnd.3 cells under hypoxic-hypoglycemic conditions, thereby restoring tight junctions. Additionally, our elucidates critical role NF-κB signaling pathway amelioration PRP-Exos. In model, local injection hydrogel-encapsulated reduced Evans blue dye leakage, enhanced expression junction proteins, alleviated inflammatory environment damaged area, improved recovery. conclusion, presents a promising effective treatment option for SCI.
Language: Английский
Citations
5
European Journal of Pharmacology, Journal Year: 2025, Volume and Issue: 992, P. 177349 - 177349
Published: Feb. 5, 2025
Spinal cord injury (SCI) is a devastating event for the central nervous system (CNS), often resulting in loss of sensory and motor functions. It profoundly affects both physiological psychological well-being patients, reducing their quality life while also imposing significant economic pressure on families healthcare system. Due to complex pathophysiology SCI, effective treatments promoting recovery remain scarce. Mesenchymal stem cell-derived exosomes (MSC-Exos) offer advantages such as low immunogenicity, good biocompatibility, ability cross blood-spinal barrier (BSCB). In preclinical studies, they have progressively shown efficacy SCI repair functional recovery. However, yield insufficient targeting MSC-Exos limit therapeutic efficacy. Currently, genetic engineering other preprocessing techniques are being employed optimize properties exosomes, thereby enhancing potential. Therefore, this paper provides an overview biogenesis exosomes. summarizes current approaches optimizing exosome performance. Additionally, it details mechanisms through which optimized provide neuroprotection explores potential combined involving hydrogels.
Language: Английский
Citations
0
Molecular Neurobiology, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 26, 2025
Language: Английский
Citations
0
Neurochemical Research, Journal Year: 2025, Volume and Issue: 50(2)
Published: March 4, 2025
Language: Английский
Citations
0
Phytotherapy Research, Journal Year: 2025, Volume and Issue: unknown
Published: April 2, 2025
Spinal cord injury (SCI) is a serious neurological disease with an extremely high disability rate. Most patients show loss of motor and sensory functions below the level injury. Current treatment protocols are based on early surgical decompression pharmacotherapy. However, efficacy these interventions suboptimal. Due to its complex pathophysiological mechanisms difficulty central nervous system (CNS) regeneration, exploring effective therapeutic remains daunting. Flavonoids secondary metabolites unique plants that have attracted attention in recent years for their potential now commonly used inflammation, tumors, other diseases. For SCI, related studies still exploring; some compounds, such as quercetin, fisetin, hesperetin, shown good anti-inflammatory anti-apoptotic properties, which help restore function injured spinal cord. flavonoids exhibit certain disadvantages, including poor solubility, low bioavailability, inability achieve long-term controlled release. Some proposed drug delivery strategies-including nanoparticles, hydrogels, collagen scaffolds-to enhance efficacy. In this paper, we summarize strategies SCI by searching relevant literature propose future research directions provide new ideas multimodal SCI.
Language: Английский
Citations
0
Neurochemical Research, Journal Year: 2024, Volume and Issue: 49(8), P. 2120 - 2130
Published: May 31, 2024
Language: Английский
Citations
3
Journal of Clinical Medicine, Journal Year: 2024, Volume and Issue: 13(10), P. 2773 - 2773
Published: May 8, 2024
Spinal cord injury is a complicated medical condition both from the clinician's point of view in terms management and patient's perspective unsatisfactory recovery. Depending on severity, this disorder can be devastating despite rapid appropriate use modern imaging techniques convenient surgical spinal decompression stabilization. In context, there mandatory need for novel adjunctive therapeutic approaches to classical treatments improve rehabilitation chances clinical outcomes. This review offers new original therapies targeting microglia, one most relevant immune cells implicated disorders. The first part manuscript reviews anatomical pathophysiological importance blood-spinal barrier components, including role microglia post-acute neuroinflammation. Subsequently, authors present emerging based modulation, such as cytokines modulators, stem cell, microRNA, nanoparticle-based that could positively impact management. Finally, future perspectives challenges are also highlighted ongoing trials related medications microglia.
Language: Английский
Citations
2
Neurochemistry International, Journal Year: 2024, Volume and Issue: 178, P. 105801 - 105801
Published: July 5, 2024
Language: Английский
Citations
2
Cells, Journal Year: 2024, Volume and Issue: 13(22), P. 1834 - 1834
Published: Nov. 6, 2024
Microglia, the resident immune cells of central nervous system (CNS), play a crucial role in maintaining neural homeostasis but can also contribute to disease and injury when this state is disrupted or conversely pivotal neurorepair. One way that microglia exert their effects through secretion small vesicles, microglia-derived exosomes (MGEVs). Exosomes facilitate intercellular communication transported cargoes proteins, lipids, RNA, other bioactive molecules alter behavior internalize them. Under normal physiological conditions, MGEVs are essential homeostasis, whereas dysregulation production and/or alterations have been implicated pathogenesis numerous neurodegenerative diseases, including Alzheimer's (AD), Parkinson's (PD), multiple sclerosis (MS), spinal cord (SCI), traumatic brain (TBI). In contrast, may offer therapeutic potential by reversing inflammation being amenable engineering for delivery beneficial biologics drugs. The determined phenotypic parent microglia. from anti-inflammatory pro-regenerative support neurorepair cell survival delivering neurotrophic factors, mediators, molecular chaperones. Further, deliver components like mitochondrial DNA (mtDNA) proteins damaged neurons enhance cellular metabolism resilience. derived pro-inflammatory detrimental on health. Their cargo often contains cytokines, involved oxidative stress, neurotoxic which exacerbate neuroinflammation, neuronal damage, impair synaptic function, hindering processes. neurodegeneration injury-whether harmful-largely depends how they modulate pro- factors cargo, cytokines microRNAs. addition, propagation pathological such as amyloid-beta alpha-synuclein, progression disorders AD PD, transfer apoptotic necrotic induce neuron toxicity trigger glial scarring during neurological injury. review, we provided comprehensive up-to-date understanding mechanisms underlying multifaceted disease. particular, specific exosome various either recovery, will be discussed. has highlighted methodologies employed cell-selective targeting. Understanding influence balance between signaling CNS developing new strategies diseases neurotrauma.
Language: Английский
Citations
2