Hierarchical Interconnected Porous Scaffolds with Regulated Interfacial Nanotopography Exhibit Antimicrobial, Alleviate Inflammation, Neovascularization, and Tissue Integration for Bone Regeneration

Biomaterials, Journal Year: 2025, Volume and Issue: 318, P. 123186 - 123186

Published: Feb. 13, 2025

Language: Английский

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Exosomes in cartilage microenvironment regulation and cartilage repair

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 13

Published: March 5, 2025

Osteoarthritis (OA) is a debilitating disease that predominantly impacts the hip, hand, and knee joints. Its pathology defined by progressive degradation of articular cartilage, formation bone spurs, synovial inflammation, resulting in pain, joint function limitations, substantial societal familial burdens. Current treatment strategies primarily target pain alleviation, yet improved interventions addressing underlying are scarce. Recently, exosomes have emerged as subject growing interest OA therapy. Numerous studies investigated to offer promising therapeutic approaches for through diverse vivo vitro models, elucidating mechanisms which from various cell sources modulate cartilage microenvironment promote repair. Preclinical investigations demonstrated regulatory effects originating human cells, including mesenchymal stem cells (MSC), fibroblasts, chondrocytes, macrophages, derived Chinese herbal medicines, on modulation repair signaling pathways. Additionally, encompass matrix, proliferation migration autophagy, apoptosis, mitigation oxidative stress. An increasing number exosome carrier scaffolds under development. Our review adopts multidimensional approach enhance comprehension pivotal functions exerted sourced types OA. Ultimately, our aim pinpoint targets capable regulating facilitating

Language: Английский

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Exploring NamiRNA networks and time-series gene expression in osteogenic differentiation of adipose-derived stem cells

Annals of Medicine, Journal Year: 2025, Volume and Issue: 57(1)

Published: March 18, 2025

Background Adipose-derived stem cells (ADSCs) are a type of cell found in adipose tissue with the capacity to differentiate into multiple lineages, including osteoblasts. The differentiation ADSCs osteoblasts underlies osteogenic and pathological cellular basis osteoporosis, bone damage repair.

Language: Английский

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Periprosthetic osteolysis: Mechanisms and potential treatment strategies

Cellular Signalling, Journal Year: 2025, Volume and Issue: unknown, P. 111758 - 111758

Published: March 1, 2025

Language: Английский

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Magnetic Scaffolds for the Mechanotransduction Stimulation in Tendon Tissue Regeneration

Materials Today Bio, Journal Year: 2025, Volume and Issue: unknown, P. 101699 - 101699

Published: March 1, 2025

Nowadays, tendon injuries represent a global health issue that annually affects millions of individuals. An innovative approach for their treatment is represented by the development tissue engineered scaffolds able to support host cells adhesion, differentiation, and proliferation. However, scaffold alone could be insufficient guarantee an improvement healing control. Magnetite nanoparticles (Fe3O4 NPs) are gaining interest due unique properties. In particular, when combined with bio-mimetic scaffolds, they should lead mechano-stimulation, improving tenogenic differentiation allowing deeper reparation. The aim this work study based on polyhydroxybutyrate gelatin doped Fe3O4 NPs. characterized aligned fibrous shape mimic fascicles. Moreover, possess superparamagnetic behavior slow degradation rate structural during regeneration. magnetic promote cell proliferation alignment onto matrix, in particular application external field. Also, differentiate produce collagen I extracellular matrix. Finally, vivo promotes complete after 1 week stimulation.

Language: Английский

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Orthobiologics Revisited: A Concise Perspective on Regenerative Orthopedics

Current Issues in Molecular Biology, Journal Year: 2025, Volume and Issue: 47(4), P. 247 - 247

Published: April 2, 2025

At the forefront of regenerative medicine, orthobiologics represent a spectrum biological substances that offer promising alternatives for tissue repair and regeneration. Traditional surgical treatments often involve significant risks, extended recovery periods, may not fully restore functionality, creating strong demand less invasive options. This paper presents concise overview orthobiologics, reexamining their role within broader landscape medicine. Beginning with brief introduction to navigates through various types materials associated mechanisms action clinical applications. By highlighting platelet derivatives, bone marrow-derived products, processed adipose tissue, among others, it underscores pivotal in prompting responses like cellular proliferation, differentiation, angiogenesis, thereby fostering healing. Furthermore, this explores diverse applications orthopedic conditions, outlining utility treatment soft-tissue injuries. Addressing considerations, discusses safety profiles, efficacy, patient selection criteria, emerging challenges. With limitations traditional medicine becoming more apparent, an innovative approach care. Looking forward, approaches future directions research, emphasizing need continued innovation exploration. Through perspective, aims provide clinicians, researchers, stakeholders comprehensive understanding evolving

Language: Английский

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Adipose stromal cells in the human rotator cuff are resistant to fibrotic microenvironmental cues

The Journal of Physiology, Journal Year: 2025, Volume and Issue: unknown

Published: April 8, 2025

Abstract Rotator cuff tears are the most common upper extremity orthopaedic injury, causing degenerative changes within bone, tendon, joint capsule, bursa and muscle. These linked to poor rehabilitative surgical outcomes, which has launched investigations into co‐therapeutic biologics. Specifically, mesenchymal stem cells (MSCs) have shown promise in mitigating animal models of rotator tears, but reports their impact on clinical outcomes remain mixed. Here we describe an alternative source MSCs human shoulder, adipose stromal (ASCs) from subacromial fat (SAF) pad. Compared gold‐standard subcutaneous (SQ) fat, show that SAF ASCs less sensitive chemical mechanical fibrotic cues, (1) retaining smaller cell area with reduced actin stress fibre alignment across a range physiological pathological stiffnesses, (2) having traction forces extracellular matrix production, (3) myofibroblastic conversion response cytokine challenge. Furthermore, enhance fusion primary myoblasts via paracrine signalling. Despite signature cuffs tendon sourced torn were equally effective at resisting fibroblastic promoting myogenesis as those intact cuffs, further supporting autologous use these cells. In conclusion, this study describes alternative, potentially superior, for therapies. image Key points Adipose tissue is novel understudied therapeutic Here, detail its resident cells, make comparison shoulder cause fibrosis tissue; does not downstream morphology or pro‐myogenic signaling. resist microenvironmental cues enhanced signaling compared traditional represent new type can be impactful advancing

Language: Английский

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Stem cell therapy for intervertebral disc degeneration: Clinical progress with exosomes and gene vectors

World Journal of Stem Cells, Journal Year: 2025, Volume and Issue: 17(4)

Published: April 21, 2025

Intervertebral disc degeneration is a leading cause of lower back pain and characterized by pathological processes such as nucleus pulposus cell apoptosis, extracellular matrix imbalance, annulus fibrosus rupture. These changes result in height loss functional decline, potentially to herniation. This comprehensive review aimed address the current challenges intervertebral treatment evaluating regenerative potential stem cell-based therapies, with particular focus on emerging technologies exosomes gene vector systems. Through mechanisms differentiation, paracrine effects, immunomodulation, cells facilitate repair reduce apoptosis. Despite recent advancements, clinical applications are hindered hypoxic environments immune rejection. By analyzing preclinical findings, this provided insights into optimizing therapy overcome these obstacles highlighted future directions field.

Language: Английский

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Adipose-Derived Stem Cell Exosomes for Stress Urinary Incontinence: A Novel in Vivo Therapeutic Approach

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

This study aimed to evaluate the therapeutic effects of Adipose tissue-derived stem cell exosomes (ADSCE) in management stress urinary incontinence (SUI) using vaginal dilatation-induced acute and chronic SUI models Sprague Dawley rats. Flow cytometry confirmed mesenchymal identity isolated UC-SCs, while exosomal markers (CD9, CD63, CD81) validated successful exosome isolation. Transmission electron microscopy revealed characteristic nanoscale morphology exosomes, further supported by energy-dispersive X-ray analysis. Therapeutic efficacy was assessed through urodynamic histopathological analyses. Abdominal leak point pressure (ALPP) significantly reduced both compared controls (P < 0.0001). ADSCE therapy increased ALPP, with systemic administration demonstrating superior over local treatment 0.05). Histopathological examination indicated substantial sphincter muscle thinning, edema, fibrosis untreated models, mitigated these pathological changes. Masson’s Trichrome staining significant preservation urethral thickness treated groups 0.01), yielding moderate improvements administration. Furthermore, markedly collagen deposition, particularly groups, suggesting an antifibrotic effect enhanced tissue remodeling. These findings indicate that effectively restores function mitigates alterations SUI. Systemic demonstrates potential, highlighting as a promising regenerative strategy for management.

Language: Английский

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Role of Non-Coding RNAs in White and Brown Adipose Tissue Differentiation and Development

Non-Coding RNA, Journal Year: 2025, Volume and Issue: 11(3), P. 30 - 30

Published: April 29, 2025

Adipocyte differentiation is a complex process in which pluripotent mesenchymal stem cells (MSCs) differentiate and develop into mature fat cells, also known as adipocytes. This controlled by various transcription factors, hormones, signaling molecules that regulate the development of these cells. Recently, an increasing number non-coding RNAs (ncRNAs), especially microRNAs (miRNAs), have been established to be involved regulation many biological processes, including adipocyte differentiation, development, metabolism, energy homeostasis white brown adipose tissue. Several vitro vivo studies reported significant role ncRNAs either promoting or inhibiting targeting specific factors regulating expression key adipogenic genes. Identifying function their subsequent targets contributes our understanding how can used potential biomarkers tools for therapies against obesity, diabetes, other diseases related obesity. could contribute advancements tissue-engineering based treatments. In this review, we intended present up-to-date comprehensive literature overview ncRNAs, miRNAs, long (lncRNAs), circular (circRNAs), focusing particularly on addition, further discuss use novel therapeutic strategies future personalized treatment options patients.

Language: Английский

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