Inflammatory Modulation of Toll-like Receptors in Periodontal Ligament Stem Cells: Implications for Periodontal Therapy DOI Creative Commons
Mohamed Mekhemar,

Immo Terheyden,

Christof E. Dörfer

et al.

Cells, Journal Year: 2025, Volume and Issue: 14(6), P. 432 - 432

Published: March 13, 2025

Toll-like receptors (TLRs) play a crucial role in the innate immune response, mediating cellular interactions with microenvironment and influencing periodontal disease progression. This vitro study aimed to comprehensively characterize TLR expression profile of ligament mesenchymal stem/progenitor cells (PDLSCs) investigate its modulation by inflammatory stimuli associated disease. PDLSCs (n = 6) were isolated, selected using anti-STRO-1 antibodies, cultured evaluate their colony-forming abilities characteristics. Baseline inflammation-induced expressions evaluated RT-PCR protein analyses following cytokine-mediated stimulation. exhibited expected stem cell characteristics expressed multiple TLRs under both conditions. Notably, stimulation significantly upregulated TLR1 TLR2 while downregulating TLR10 (p < 0.05). These findings provide comprehensive characterization demonstrate how inflammation modulates profile. The observed shifts may influence PDLSC responses microbial pathogens impact immunomodulatory regenerative properties tissues. Understanding these could contribute developing targeted strategies for improving PDLSC-based therapies

Language: Английский

Melatonin regulation and the function of the periodontal ligament: Future perspective and challenges DOI
Andrea Scribante

World Journal of Stem Cells, Journal Year: 2025, Volume and Issue: 17(1)

Published: Jan. 17, 2025

The present article reviews the emerging role of melatonin (MT) and Hippo-Yes-associated protein signaling pathway in periodontal regeneration, highlighting their potential to delay aging process ligament stem cells (PDLSCs). Oxidative stress cellular senescence are major obstacles regenerative therapies, especially an population. MT, a potent antioxidant, restores morphology, proliferation, osteogenic differentiation PDLSCs under oxidative conditions. Recent research highlights how MT enhances PDLSC stemness by upregulating Yes-associated expression, offering promising therapeutic strategy antagonize tissue degeneration. In addition, discusses growing interest probiotics as complementary approach improve oral microbiota support regeneration. integration with traditional novel approaches may pave way for innovative preventive or active treatments periodontology, aimed at reducing stress. Future needs focus on translating these findings into clinical applications promoting deeper understanding regeneration aging.

Language: Английский

Citations

0

Inflammatory Modulation of Toll-like Receptors in Periodontal Ligament Stem Cells: Implications for Periodontal Therapy DOI Creative Commons
Mohamed Mekhemar,

Immo Terheyden,

Christof E. Dörfer

et al.

Cells, Journal Year: 2025, Volume and Issue: 14(6), P. 432 - 432

Published: March 13, 2025

Toll-like receptors (TLRs) play a crucial role in the innate immune response, mediating cellular interactions with microenvironment and influencing periodontal disease progression. This vitro study aimed to comprehensively characterize TLR expression profile of ligament mesenchymal stem/progenitor cells (PDLSCs) investigate its modulation by inflammatory stimuli associated disease. PDLSCs (n = 6) were isolated, selected using anti-STRO-1 antibodies, cultured evaluate their colony-forming abilities characteristics. Baseline inflammation-induced expressions evaluated RT-PCR protein analyses following cytokine-mediated stimulation. exhibited expected stem cell characteristics expressed multiple TLRs under both conditions. Notably, stimulation significantly upregulated TLR1 TLR2 while downregulating TLR10 (p < 0.05). These findings provide comprehensive characterization demonstrate how inflammation modulates profile. The observed shifts may influence PDLSC responses microbial pathogens impact immunomodulatory regenerative properties tissues. Understanding these could contribute developing targeted strategies for improving PDLSC-based therapies

Language: Английский

Citations

0