A novel perspective on the regulation of cardiac cell beating: cardiac cell under mechanical stimulation acts as “cell activation button” to activate adjacent cardiac cell

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: Feb. 25, 2025

The regulation of cardiac cell beating is great significance for understanding coordination mechanisms and the treatment cardiovascular diseases. Inspired by this natural "cell regulates cell" mode in which sinoatrial node cells regulate atrial myocytes, study presented a novel method to replicate behavior vitro through mechanical stimulation. Primary from Sprague-Dawley rats were isolated, cultured 2D substrates, applied precise stimulation developing micro-manipulation platform. We demonstrated that probe can act as an external activation device quiescent cells, transforming them into "activation cells" capable activating adjacent "target bioelectrical coupling. Calcium imaging with Fluo-4 probes revealed activates mechanism relies on mechano-electric feedback calcium-mediated signal propagation via junctions. Our findings provide non-destructive strategy target cell, deepen insights modulation intercellular communication, offer framework studying arrhythmias linked abnormal cell-cell communication. This work combined intervention biological signaling, advancing potential applications therapeutics.

Language: Английский

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Advances in Drug Delivery Integrated with Regenerative Medicine: Innovations, Challenges, and Future Frontiers

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(4), P. 456 - 456

Published: April 1, 2025

Advances in drug delivery systems adapted with regenerative medicine have transformed healthcare by introducing innovative strategies to treat (and repair many instances) disease-impacted regions of the human body. This review provides a comprehensive analysis latest developments and challenges integrating technologies medicine. Recent advances technologies, including design biomaterials, localized techniques, controlled release guided mathematical models, are explored illustrate their role enhancing therapeutic precision efficacy. Additionally, approaches analyzed, focus on extracellular matrix components, stem cell-based therapies, emerging for organ regeneration both soft hard tissue vitro model engineering. In particular, also discusses applications cellular cells, immune endothelial specialized cells such as chondrocytes osteoblasts, highlights advancements cell methods cell–cell interaction modulation. addition, future directions pivotal trends emphasizing importance interdisciplinary collaboration cutting-edge innovations provided address successful outcomes

Language: Английский

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Gene Expression Profile of Cultured Human Coronary Arterial Endothelial Cells Exposed to Serum from Chronic Kidney Disease Patients: Role of MAPK Signaling Pathway

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3732 - 3732

Published: April 15, 2025

Patients with end-stage renal disease (ESRD) are at increased risk of cardiovascular (CVD), such as myocardial infarction (MI). Uremic toxins and endothelial dysfunction central to this process. In exploratory study, we used the Affymetrix GeneChip microarray investigate gene expression profile in uremic serum-induced human coronary arterial cells (HCAECs) from ESRD patients without MI (UWI UWOI groups) an approach its underlying mechanism. We also explored which pathways involved found 100 differentially expressed genes (DEGs) among conditions interest by supervised principal component analysis hierarchical cluster analysis. The expressions four major DEGs were validated quantitative RT-PCR. Pathway molecular network analyze interaction patterns. Ten identified main enriched metabolic according transcriptome profiling analysis, were, others, positive regulation inflammatory response, extracellular signal-regulated kinases 1 2 (ERK1/2) cascade, cardiac muscle cell development, highlighting mitogen-activated protein kinase (MAPK) activity (p = 0.00016). Up- down-regulation HCAECs exposed serum could contribute CVD patients. Our study suggests that inflammation ERK-MAPK pathway highly kidney MI, suggesting their role pathology. Further studies approaches based on MAPK interfering strategies needed confirm these data.

Language: Английский

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PEDOT: PSS-Modified Organic Flexible and Implantable Microelectrode for Internal Bi-Directional Electrophysiology of Three-Dimensional Cardiomyocyte Spheroid

ACS Sensors, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 26, 2024

Three-dimensional (3D) cardiomyocyte spheroids are essential models to replicate cardiac structural and functional features in vitro. However, conventional planar rigid microelectrode arrays (MEAs) suffer from low-quality electrophysiological recording of 3D cultures, due limited contact areas weak coupling between cells MEA chips. Herein, we developed a PEDOT: PSS-modified organic flexible implantable (OFI-MEA) coupled with self-developed integrated biosensing platform achieve high-throughput, long-term, stable bidirectional internal electrophysiology spheroids. Electrostimulation enhanced the performance cardiomyocytes, causing remarkable 2.69-fold increase frequency. Furthermore, time-frequency analysis multisite signals highlight diverse cell activity patterns It provides powerful tool record spheroids, allowing continuing evaluation dynamics regulation electrical signals, providing novel strategy for disease model construction, drug screening, cardiological research.

Language: Английский

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