The Effect of Ionising Radiation on the Properties of Tumour-Derived Exosomes and Their Ability to Modify the Biology of Non-Irradiated Breast Cancer Cells—An In Vitro Study DOI Open Access
Michał Lach, Joanna Wróblewska, Marcin Michalak

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(1), P. 376 - 376

Published: Jan. 4, 2025

The vast majority of breast cancer patients require radiotherapy but some them will develop local recurrences and potentially metastases in the future. Recent data show that exosomal cargo is essential these processes. Thus, we investigated influence ionising radiation on exosome properties their ability to modify sensitivity biology non-irradiated cells. Exosomes were isolated from cell lines (MDA-MB-231, MCF7, SKBR3) irradiated with 2 Gy (Exo Gy) or no irradiation 0 Gy). Despite differences molecular profiles, they did not affect viability, proliferation, cycle phase distribution, radioresistance; however, both populations showed migration invasion potential, as confirmed by downregulation β-catenin, which responsible for maintaining epithelial phenotype. Interestingly, exosomes BCa cells more actively deposited endothelial (EA.hy926). Furthermore, tend lower expression CD31, intact vascularity. This preliminary study demonstrates vital role altered profile due pathobiology cancer.

Language: Английский

The Effect of Ionising Radiation on the Properties of Tumour-Derived Exosomes and Their Ability to Modify the Biology of Non-Irradiated Breast Cancer Cells—An In Vitro Study DOI Open Access
Michał Lach, Joanna Wróblewska, Marcin Michalak

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(1), P. 376 - 376

Published: Jan. 4, 2025

The vast majority of breast cancer patients require radiotherapy but some them will develop local recurrences and potentially metastases in the future. Recent data show that exosomal cargo is essential these processes. Thus, we investigated influence ionising radiation on exosome properties their ability to modify sensitivity biology non-irradiated cells. Exosomes were isolated from cell lines (MDA-MB-231, MCF7, SKBR3) irradiated with 2 Gy (Exo Gy) or no irradiation 0 Gy). Despite differences molecular profiles, they did not affect viability, proliferation, cycle phase distribution, radioresistance; however, both populations showed migration invasion potential, as confirmed by downregulation β-catenin, which responsible for maintaining epithelial phenotype. Interestingly, exosomes BCa cells more actively deposited endothelial (EA.hy926). Furthermore, tend lower expression CD31, intact vascularity. This preliminary study demonstrates vital role altered profile due pathobiology cancer.

Language: Английский

Citations

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